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We narrowed to 738 results for: TRI

Showing: 181 - 200 of 738 results

  1. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ... a team to get a lot of stuff done.  Factors contributing to job satisfaction Being a good manager is ...mostly happy at work, they will work harder and contribute more. Assuming basic needs are being met (i.e...pay and benefits to live on), the factors that contribute to job satisfaction are a bit surprising. There...overwhelmingly agree that these are the factors that most contribute to job satisfaction: A feeling of being appreciated...organization and feel the importance of their contributions. Flexible work schedule Flexibility to manage...

  2. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...shut-down and the subsequent fluctuations in distribution. I don’t have space in this blog to call ...scientific community.  With the dramatic reduction in distribution, we had to figure out a way to maintain our ...operations until our normal work resumed. Addgene distributes and fulfills requests for scientists doing almost...especially proud to support Addgene now, when their distribution of plasmids for COVID-19 research is accelerating... cloning grade DNA service, and viral vector distribution is recovering from the pandemic-related slowdown...

  3. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ...Human whole genome sgRNA iBAR library Article contributed by Susanna Bachle Figure 1: Initial steps...labeling using recombinase competition Article contributed by Aliyah Weinstein Sparse Predictive Activity...reporter for calcineurin activity in yeast Article contributed by Leah Schwiesow Calcineurin is a protein phosphatase...protein fusions as a DNA staining dye Article contributed by Jennifer Tsang Figure 4: Double stranded...single molecule-fluorescence assay to monitor restriction enzyme digestion of single molecules in real-...

  4. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ... This post was contributed by guest blogger James D. Fessenden, an Assistant Professor at Brigham and... site when used in conjunction with Cy dye-nitrilotriacetic acid (NTA) conjugates (Kapanidis, Ebright,...50-69. Royal Society of Chemistry. 3. Ermolenko, Dmitri N., et al. "Observation of intersubunit movement...-mediated fluorescent labeling with (Ni2+: nitrilotriacetic acid) n-fluorochrome conjugates." Journal ...

  5. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...immune response from the body. Several factors contribute to the immunogenicity of a drug, including the... body recognizes a drug as a foreign antigen, triggering a humoral immune response. ADAs can inactivate...one of the more common open-access tools, while EpiMatrix is a commercially available algorithm to evaluate...in silico assessments, as well as analyze the contributing factors that in silico prediction algorithms...monocytes. PBMCs can be further differentiated into dentritic cells or macrophages through cytokine induction...

  6. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...In addition to single plasmids, Addgene also distributes pooled plasmid libraries containing hundreds,...versatile offerings! We recently re-amplified our distribution stock of the Brunello Human gRNA library, and...processes we use to ensure high-quality library distribution. You can also use these tips as a starting point... scaled to 1, to create a “cumulative wealth distribution” curve, or Lorenz curve. By calculating the ...gain a sense of the uniformity of the library distribution, with an AUC of 0.5 representing perfect uniformity...

  7. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ... is a silica matrix fixed in a column and you flow lysate and buffers through the matrix, usually by centrifugation... can inhibit important reactions like PCR or restriction digests. Transfection Grade DNA (high quality... centrifugation or vacuum. In a bead-based approach, you mix specially coated magnetic beads into your...constructs – Large plasmids, cosmids, or BACs, can be tricky to purify due to their size. Some manufacturers...a good option for you, some manufacturers offer trial or sample sizes of their kits, which you can use...

  8. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...This post was contributed by Mary Tamer from BioBuilder, an innovative nonprofit bringing the study of... new for themselves and when they effectively contribute as part of a team.” As a seasoned educator, Kuldell...almost every state in the U.S. and in more than 30 countries around the world. Snapshots of the BioBuilder ...open-access curriculum begins with students reading comic strips and watching animations that present a specific... lab materials and reagents such as droppers, centrifuge tubes, circuit parts, and lab-strains of bacteria...

  9. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    Published
    July 6, 2017, 1:59 p.m.
    ...presentation, starvation or the use of caloric restriction mimetics trigger autophagy and, in a mouse model led ...deposit and distribution service! References: 1. Pietrocola, Federico, et al. "Caloric restriction mimetics...from a state of chronic hepatitis caused by hypernutrition and alcohol consumption. Chronic inflammation...

  10. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type
    Blog Post
    Published
    June 6, 2019, 12:41 p.m.
    ...Light-mediated block of synaptic transmission Article contributed by Klaus Wanisch  Listen to the optogenetics ...imaging of microbes in mammalian hosts Article contributed by Lukas Morgan  Listen to the acoustic reporter...and create ultrasound contrast. At a very low volumetric density and with a resolution of less than 100...mechanisms of miRNA-mediated gene regulation Article contributed by Michael Lemieux  Listen to the miRNA reporter...vivo using the CRISPR-Cas9 system CHIME Article contributed by Aliyah Weinstein  Listen to the CRISPR podcast...

  11. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    Published
    Aug. 5, 2021, 12:15 p.m.
    ...He has recently developed a third-generation intensiometric glutamate sensing fluorescent reporter (iGluSnFR3...to identify cell populations in the brain that contribute to the development of behavioral signs of opioid...connectivity patterns of these cells and the functional contribution to a variety of behaviors related to addiction...expressed in a mammalian cell line engineered to be electrically excitable. The mutants are co-expressed with...membrane voltage,” says Tian. Figure 5: Citrine-tagged voltage indicator expressed in mouse cortex...

  12. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    Published
    Feb. 28, 2019, 1:34 p.m.
    ...collaborative group, she also joined early on and contributed her expertise in developing reproducibility workshops...Joanne Kamens, Addgene also had the opportunity to contribute to this open science collaboration. The first...workshop at the conference, but because each contributor made the materials accessible, anyone could present...present the material. For example, Addgene’s contributions by Tyler Ford included reagent sharing and were... upcoming conference or you’d like to join or contribute to this collaboration, please check out the official...

  13. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...worry about and that is something to recognize! Distributing plasmids, viral service, and more For the ...about succeeding at our core mission. Plasmid distribution is amazingly still growing at >9% every year...since 2005. We’re meeting the challenges of DNA distribution to ensure scientists everywhere can access plasmids... it. Almost 10,000 viral vector samples were distributed in 2018 and we are poised for more offerings....Addgene. There’s so much potential for service and contribution! Leadership On an organizational level, ...

  14. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...The post was contributed by guest blogger Londa Schiebinger, PhD, Hinds Professor of History of Science...the US Women’s Health Initiative Hormone Therapy Trial, for example, found that the study saved lives. ...was $35.2 billion (or $140 per $1 invested in the trial). The goal of gendered innovations It is crucially...research is minimal compared to that of human clinical trials. And, as Janine Clayton points out, late-stage ...because sex was not analyzed) may harm humans or contribute to failures in drug development – costs far greater...

  15. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ... by a variety of methods such as ultracentrifugation, centrifugation on a sucrose gradient, spin columns...producing and using lentivirus and offer some tips and tricks for overcoming these hurdles. Talking 'bout my...relatively inexpensive and does not require an ultracentrifuge, PEG will precipitate all proteins in a solution...high-titer lentivirus preparations without ultracentrifugation. Sci Rep. 2015; 5:13875. PubMed PMID: 26348152...

  16. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ...they are transferrable across most, if not all, industries and work environments. This is why they are called... bathroom mirror pep talk, forgotten and then retrieved your lucky socks, persevered through a nerve wracking...potential teammates want to be assured that you will contribute openness, clarity, approachability, and professionalism...interview, check out this article from The Muse. Kai Strickland is a former Customer Support and Operations Manager... Kai Strickland...

  18. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...dependencies they identified and their associated ancestries. While there were some exciting relationships...Dependency Map) than lines from European or East Asian ancestries. These SNPs, however, were not accounted for ...racial bias. It is easy, even unintentionally, to attribute to an ancestry group what is actually an artifact...and references References Germline variation contributes to false negatives in CRISPR-based experiments...experiments with varying burden across ancestries. Sean A Misek, Aaron Fultineer, Jeremie Kalfon, Javad Noorbakhsh...

  19. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...generally referred to as Antigen Retrieval. I chose not to get into antigen retrieval here because it’s not quite...permeabilization reagents are detergents, such as Triton X-100, Tween-20, or saponin. Each detergent has...membrane-associated proteins in place. In contrast, Triton X-100 and Tween-20 are examples of non-ionic detergents...Permeabilizing with a non-ionic detergent, like Triton X-100, can lose membrane-associated proteins, but...

  20. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...controlled by collections of neurons that are intricately wired into circuits through synaptic connections...weakly expressed. As these epitopes tags have no intrinsic visual signal, they cannot be detected directly...neurons. As compared to epitopes tags, FPs are intrinsically fluorescent and can therefore be used in live... interactions. Also, overexpression of FPs can trigger protein aggregation which is toxic for the cells...darkened chromophores. These probes are both intrinsically fluorescent and can be detected with highly ...
Showing: 181 - 200 of 738 results