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We narrowed to 484 results for: Vit

Showing: 181 - 200 of 484 results

  1. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ... So never mind how much better your work and productivity will be if you’re part of a team - it will just...That’s a subtle but important distinction. If I invite someone to be on a project team, I try to make ...next team works better. In fact, don’t wait to be invited onto a team. Make one yourself. Instead of thinking...

  2. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ... the known pathway that were essential for the activity of different ligands. Such knowledge could be ...effects. Typically the phenotype would be studied in vitro, but there is enormous potential in performing in...G., et al. "Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9...

  3. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ... influence receptor conformation and signaling activity​​ (Sridharan et al., 2014). It's also crucial ...addition of fluorophores affects the functional activity of the ligands. For instance, studies have shown...molecules (<10nm) and overlap of excitation profiles is vital for the accurate interpretation of FRET-based experiments...

  4. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...exposed ssDNA bases. Thus, base editors have an activity window restricted to the stretch of ssDNA created... edits at certain positions within the editing activity window (Kurt et al., 2021). They hypothesized ...suggest that DSBs are a common intermediate of CGBE activity, and they are sometimes processed by error-prone...

  6. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ... a weak signal. However, they have high cross-reactivity, vary significantly from lot-to-lot, and have...but they have higher specificity, lower cross-reactivity, and are more consistent lot-to-lot. The highest...usually preferred because of their higher specific activity, slightly lower cost, and smaller size. However...

  7. #DeckTheLab with Tinsel and Test Tubes, Fa La La La La La La La La

    Type
    Blog Post
    Published
    Dec. 2, 2021, 2:15 p.m.
    ...up at home!This year with a #DeckTheLab themed nativity scene, courtesy of our house pets.. 😁 pic.twitter.com... fun, be creative, and enjoy the end of year festivities - however you celebrate! And if you want to see...

  8. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    Published
    May 2, 2024, 1:15 p.m.
    ...FITS In vitro CRISPR screens in immune cells have identified many putative regulators of immunity. However...However, in vitro screens cannot faithfully model the complex spatial, cellular, metabolic, and cytokine...

  9. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    Published
    Oct. 31, 2019, 1:06 p.m.
    ...ways to incorporate science themes into our fun activities and Halloween is no exception. For the past 10...grasshoppers, including those linked to neurotransmitter activity and geotactic behavior (Biron et al., 2005).   ...

  10. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...microscopy, the team reveal the mechanism behind its activity including an extensive RNA scaffold and a domain...hydroxytamoxifen, it can move to the nucleus for activity. They also developed one of these systems further...

  11. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Jan. 23, 2024, 2:15 p.m.
    ...feels both welcomed and resourced for success. I invite you to join me here, and on my LinkedIn page, as...perspective on Addgene through her LinkedIn page. She invites you to connect with her there. More on the Addgene...

  12. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...single day. CUT&RUN works by using the DNA cutting activity of a Protein A fused micrococcal nuclease (MNase...calcium, which MNase requires for its nuclease activity and is chelated from the reaction up until this...

  13. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...neuroscience by granting targeted control over neuronal activity. While they serve similar purposes, both techniques...or track the exact time of activation. Receptor activity is also drawn out as drugs can remain present ...

  15. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ... really, the possibilities are endless.    Activity Example audience  Talking about your career...defining your audience for science communication activities  Many people are concerned that by defining a...

  17. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ... into XP genes and other genes involved in photosensitivity in melanocytes with the goal of understanding...basal conditions normally after periods of high activity. The new work will help them to understand how...

  19. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ... continue to be useful for monitoring neuronal activity in the form of calcium dynamics. (For more details...fluorescence) and is useful for imaging signaling activity in large populations of densely-labeled neurons...

  20. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ...to increase fluorescence 10-fold after protease activity (To et al., 2016). However, the lab wanted to ...spatial and temporal dynamics of apoptosis both in vitro and in living organisms to better study and understand...
Showing: 181 - 200 of 484 results