We narrowed to 235 results for: bon;

1. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

   Type

   Blog Post

   Published

   Dec. 12, 2013, 3:35 p.m.

   ...what do you want to make?   References: Jerome Bonnet et al. "Amplifying genetic logic gates." Science...

2. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

   Type

   Blog Post

   Published

   Dec. 3, 2019, 11:38 a.m.

   .... S. cerevisiae) Plasmid Type: Choose an empty backbone if you intend to clone your own insert, or use...

3. Isolating Genomic Regions of Interest with the CRISPR System

   Type

   Blog Post

   Published

   May 30, 2019, 12:44 p.m.

   ...enCh IP technology using recombinant CRISPR ribonucleoproteins." Genes to Cells 21.4 (2016): 370-377. PubMed...

4. Simplify Cloning with in vivo Assembly

   Type

   Blog Post

   Published

   Oct. 18, 2018, 12:37 p.m.

   ...immediate co-transformation with linearized vector backbone. DpnI digestion - 1 μl DpnI is added to the...

5. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

   Type

   Blog Post

   Published

   Aug. 27, 2020, 1:15 p.m.

   ...is irreversible — FP fragments become covalently bonded by associating with one another — which means it...

6. Antibodies 101: Producing Recombinant Antibodies

   Type

   Blog Post

   Published

   Aug. 13, 2024, 1:15 p.m.

   ...periplasmic space to be oxidized and form disulfide bonds (Frenzel et. al., 2013). If you’re using a mammalian...

7. Plasmids 101: Secondary Nanobody Toolbox

   Type

   Blog Post

   Published

   Feb. 27, 2018, 2:04 p.m.

   ...Maleimide-labeling occurs when a maleimide group forms a covalent bond with a sulfhydryl group (–SH), such as that found...

8. DNA Purification Without a Kit

   Type

   Blog Post

   Published

   Feb. 11, 2020, 2:15 p.m.

   ...extraction protocol) disrupts the DNA’s hydrogen bonds with water molecules and coaxes it into sticking...

9. Cpf1: A New Tool for CRISPR Genome Editing

   Type

   Blog Post

   Published

   Oct. 14, 2015, 2:30 p.m.

   ...eSpCas9 and SpCas9-HF1. Both AsCpf1 and LbCpf1 ribonucleoproteins (RNPs) failed to induce off-target editing...

10. Plasmids 101: Protein tags

    Type

    Blog Post

    Published

    Dec. 11, 2014, 4:26 p.m.

    ...such as Thioredoxin A (TrxA) that improve disulfide bond formation in order to help keep your protein soluble...

11. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type

    Blog Post

    Published

    Dec. 2, 2014, 7:23 p.m.

    ...addition, we have recently developed the oligoribonucleotide (ORN) interference-PCR (ORNi-PCR) method ...

12. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type

    Blog Post

    Published

    May 15, 2014, 3:33 p.m.

    ...Click here to view Addgene’s collection of empty backbones for constructing fluorescent fusions. Transcription...

13. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type

    Blog Post

    Published

    May 2, 2019, 12:41 p.m.

    ...interesting behaviors. There is diversity in the social bonds that they form including genetic mechanisms that...

14. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type

    Blog Post

    Published

    Nov. 1, 2018, 12:35 p.m.

    ...toxin-antitoxin system is MazE/MazF. The toxin MazF is a ribonuclease that cleaves specific sequences in RNA resulting...

15. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type

    Blog Post

    Published

    Oct. 13, 2022, 1:45 p.m.

    ...making it a more flexible cutter with the added bonus of low off-target effects. The SpG variant, discovered...

16. Antibodies 101: Immunoprecipitation

    Type

    Blog Post

    Published

    Dec. 7, 2021, 2:15 p.m.

    ...differing amounts of target proteins or antibody bonding strengths.    . Steps of an IP: 1) Protein...

17. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type

    Blog Post

    Published

    Oct. 19, 2017, 1:54 p.m.

    ...PMC41617. 7. Metzger, D, J Clifford, H Chiba, and P Chambon. 1995. “Conditional Site-Specific Recombination...

18. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

    Type

    Blog Post

    Published

    Sept. 9, 2025, 1:15 p.m.

    .... These sequences were then cloned into an AAV backbone containing a minimal promoter to drive expression...

19. Plasmids 101: Shuttle Vectors

    Type

    Blog Post

    Published

    May 20, 2025, 1:15 p.m.

    ...Resources on Addgene.org Collection of empty plasmid backbones ...

20. Viral Production at Addgene

    Type

    Blog Post

    Published

    Oct. 24, 2023, 1:15 p.m.

    ...responsive element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a control for...