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We narrowed to 535 results for: epo

Showing: 181 - 200 of 535 results

  1. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ...Addgenies, we’re able to feature some recently deposited plasmids every quarter. The “hot plasmids” from...Academic Job Interview by Erik Snapp Addgene depositor and guest blogger Erik Snapp breaks down how to... blog posts Resources at Addgene Browse the repository Find educational resources at Addgene Search ...

  2. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ...522 organizations in 41 countries enriched our repository with 15,020 new plasmids — a testament to the...viral vector serotypes, or hundreds of Data Hub reports detailing antibody experimental results. We also...options. Almost any AAV transfer plasmid in our repository can now be packaged on demand in five distinct...

  3. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    Published
    Jan. 16, 2024, 6:13 p.m.
    ... CHIME and new X-CHIME systems, developed and deposited at Addgene by Arlene Sharpe’s lab, allow for wide... scenarios. X-CHIME We recently developed and deposited four new systems to fill this gap. To enable context-specific...Figure from LaFleur, et al. 2024.   The X-CHIME deposit also includes pXPR_071, a plasmid that leads to...

  4. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    Published
    May 17, 2016, 2:30 p.m.
    ...performing one.  At Addgene, over 120 labs have deposited CRISPR reagents, including many gRNA-containing...derived from scientists’ submissions, either their deposited gRNA plasmids or from submission of a spreadsheet...gRNA), application, cas9 species, PubMed ID, and depositor. The CRISPR applications we currently have data...

  5. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ... successful, internationally renowned plasmid repository that it is today. Although I wish I could take... attributed to the entire Addgene family; the depositors and requesting scientists, all of the Addgene... It was great to see the faces of some of our depositing scientists who weren’t able to join us in person...

  6. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...and a domain needed for DNA unwinding. They’ve deposited plasmids for active and inactive CasX for E. coli...If you have a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in...Find CRISPR plasmids by function Find recently deposited plasmids ...

  7. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ...bailii) and B3 (from Zygosaccharomyces bisporus.) Reporter constructs equivalent to site-STOP-site-myrRFP...cross-reactivity of these recombinases, each recombinase and reporter construct pair were tested for excision efficiency...cross-reactivity; however, the other recombinase/reporter pairs were inactive, even at very high recombinase...

  8. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    Published
    July 29, 2014, 6:16 p.m.
    ...Davidson's lab to coordinate the impressively big deposit. “He has every fluorescent protein under the sun...stage IV lung cancer that has now prompted him to deposit the tools he and his team created with the research...team who have made this large, important plasmid deposit possible.   ...

  9. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    Published
    March 27, 2014, 5:14 p.m.
    ...background.” Just last month in Scientific Reports, Verkhusha’s team reported another advance in the application.... Transparency window That’s exactly what they reported doing in a paper that appeared in Nature Biotechnology...

  10. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ...bacteria, but have no fear - plasmids from Addgene depositors are making it easier than ever to use CRISPR  ...double stranded breaks (DSB) are lethal. Addgene depositor Luciano Marraffini’s lab took advantage of this...challenging problem than repression, scientists have deposited several plasmid tools that can be used in this...microbiome Beyond the human gut, the Barrick lab has deposited plasmids that can be used to engineer the bee ...

  11. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...viral infection a) antibody-dependent complement deposition (ADCD) b) antibody-dependent cellular cytotoxicity...., 2019.    Antibody-dependent complement deposition (ADCD) Antibodies can bind to a pathogen and ...opsonization. In antibody-dependent complement deposition (ADCD), opsonization of IgG and IgM antibodies...

  12. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    Published
    July 16, 2019, 12:59 p.m.
    ...positive or more negative.  Neuronal stimulation: Depolarization When the inside of the neuron becomes positive...positive over a certain threshold, it becomes depolarized and will fire a signal. The non-specific cation...both cause ion movement that results in cell depolarization. They therefore function as a neuron “on switch...

  13. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ... the most frequently requested vectors in our repository and will discuss some of the features you may...Some of the more popular studies involve using a reporter vector to tag YGOI with lacZ and/or GFP (e.g. ...animals, gene targeting and genome modifications to reporter vectors (e.g. pBV-Luc) and gene silencing (e.g...

  14. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...plasmids and to receive important coronavirus-related deposits. Our valiant lab and shipping staff agreed to ...to come in and start getting plasmid deposits into the collection and to start shipping. They created the...also moved fast to ensure that the majority of deposited materials to study SARS-CoV-2 would be fully open...

  15. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ... development of several fluorescent autophagy reporters now allows for the measurement of autophagic flux... al. "Rosella: a fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in...using a single fluorescent biosensor." Scientific reports 5 (2015): 12397. PubMed PMID: 26215030. PubMed ...

  16. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    Published
    June 17, 2021, 1:17 p.m.
    ...the DNA-binding domain of AraC using a mCherry reporter downstream of a PBAD promoter. While none of the...contains BLADE, the PBAD promoter, and a mCherry reporter downstream of the PBAD promoter. To use this plasmid...shared with Addgene. You can find all the plasmids deposited from Di Ventura's lab, including the popular light-inducible...

  17. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ... cells and activate expression of a luciferase reporter. CPP was not required for protein entry into cells...primary cell types using Cre and various loxP reporters. In both mouse and human embryonic stem cells,...Plasmids @Addgene: iTOP-tested proteins and reporters Previous Protein Delivery Techniques: Introduction...

  19. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...actively require these skills, including: Science Reporter Scientific Sales Epidemiologist Scientific Recruitment... actively use these skills include: Science Reporter Scientific Illustrator Research and Development...desirable in a range of roles, including: Science Reporter Technical Writer Statistician Bioinformatician...

  20. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...control process alone takes nearly a year. Addgene depositor Juan Carlos Izpsua Belmonte said it took the joint...the scientists I've already mentioned, Addgene depositors Marius Wernig, Kristin Baldwin, Kathrin Plath...spoken at this meeting. In fact, we have so many depositors from the stem cell community that they have their...
Showing: 181 - 200 of 535 results