We narrowed to 259 results for: human dat

...guide RNAs enhance CRISPR-Cas genome editing in human primary cells. Nat Biotechnol 33, 985–989 (2015)..., gRNAs can be chemically modified to resist degradation while still effectively serving their function...recognized as foreign RNA. To protect the RNA from degradation, several simple methods have been employed which...stability.  Nature has evolved ways to prevent degradation of important cellular RNA molecules with post...

...paper Systematic morphological profiling of human gene and allele function via Cell Painting. Rohban...mutations that are present, and use prior knowledge (data connecting each genotype with vulnerabilities) to...thus providing a starting point for downstream validation experiments. But, there have been two huge challenges... exciting plasmid collections (together with the data generated from these alleles in the above publications...

...to demonstrate that this approach was useful in humans. Then, one of them heard about our online platform...protocol can save weeks of trial-and-error. A recommendation for the correct statistical test can save a...

...collection reported to date, containing all 620 sequences of probable mature human miRNAs. Weiss’s collection...mechanisms of miRNA regulation have yet to be fully elucidated. Ron Weiss’s lab at MIT pushed the field further...

...Lemieux  Listen to the SH3 domain podcast segment The human proteome contains a breadth of modular domains that... dsRNAs target specific mRNA transcripts for degradation. Despite how easy these experiments are to physically...changes to the assay paper, so no special equipment or data analyst is necessary. How does this assay work? ...

..., Brunet E (2014) Chromosomal Translocations in Human Cells Are Generated by Canonical Nonhomologous End-Joining...Last updated Sept 1, 2020. This post was contributed by David Wyatt and Dale Ramsden, UNC at Chapel Hill...off-target sites. Christina Mork contributed to the update of this post. Thank you to our guest bloggers! ...

...my target sequence into a backbone that uses the human U6 promoter to drive expression, is it necessary...nucleotide to the start of my target sequence? A3: The human U6 promoter prefers a 'G' at the transcription start...Systems has expanded out of control. To further consolidate these FAQs, Le Cong worked with Addgene Senior...IDT (non-PAGE purified oligo is fine based on new data). For general transfection, you could use 1 µl of... al., 2013) do you have any suggestions? A15: To date, I have never used the Ex Taq. In our hands, Herculase...with Addgene. Thank you, Le! This blog post was updated to include all FAQs from Le Cong on June 24, 2025...

...synthetic biology to create tools to better understand human disease.  Outside the lab, he is interested in communicating...establish grants through the Sloan foundation for postdoc-related data collection and analysis. He is also...Future of Research Symposium Boston 2015 – Academic Data and the Labor Market. For more information on the...These comments are well correlated with published data. For example, in 2010, less than 15% of postdocs...positions available in academia (5, 6). Academic data and the labor market for PhDs Later this week, postdocs...particular, at the symposium we will discuss where better data is needed to help shape and inform the overall structure... on Friday morning will be dedicated to Academic data and the Labor Market. On this topic it is clear ...

... Methods for Monitoring Autophagy from Yeast to Human. Autophagy,3(3), 181-206. doi:10.4161/auto.3678....assays Long-lived protein degradation: This approach measures the degradation rate of radio-labeled long-lived...this is indicative of protein degradation). To control for protein degradation due to other pathways, it ...cytosol and organelles to the vacuole/lysosome for degradation and recycling. The key to Rosella’s autophagy-sensing...short-lived proteins primarily via proteasome degradation. Autophagy is then induced and quantified by ...it is standard practice to compare degradation rates between samples treated with or without an autophagy... measure of autophagy, but, like the protein degradation assay above, it’s slow and only measures bulk...

...science was performed, written and evaluated by humans. Keeping up with the literature There are a few...their data. They should explain some of the rationale behind the line of experiments, what data they collected...your search and sorting the results (relevance, dates etc). The links for the titles can generally take... an overall conclusion(s). In some articles, the data are presented to build a case for a certain model...interpretation of the results? Then think deeper about the data they are showing. Are those experiments the best...best way of figuring out what’s going on? Is the data of sufficient quality (error bars, statistics, clarity...perfect, so even though it is science’s way of validating work prior to publication, it doesn’t mean that...

...There are over 40 trillion cells that make up the human body and G-baToN provides versibility in tracking... leading to the degradation of the AID-tagged protein. To quantify protein degradation the Ward lab used... that allows for both spatiotemporal protein degradation and quantification of this depletion using imaging-based... inducible degron sequence) tagged proteins. Degradation of your protein interest would also result in...system that brings Cas9 to ubiquitin ligase for degradation.      New from the viral service By Jason ...

..., the researchers showed that expression of the human ASCL1 gene could be spatially controlled in a reversible...system in HEK293T cells to induce expression of human IL1RN, the researchers saw an 11-fold increase in...by Caroline LaManna, published Mar 8, 2016. The updated and expanded version by Nyla Naim was published...photomasking technique. Last, Hemphill et al. presented data showing that this genetically encoded, photocaged...

...: Micrographs of the biosensor expressed in the human cell line, Hep3B. From left to right: Mitotracker...protein activity and have important roles during oxidative stress. To monitor this activity, Pedre Pérez’...withdrawal Elizabeth Hubbard is a fourth year Ph.D. candidate at the University of California, Irvine where ...work on the PV neurons is in progress, she has validated the dual virus method to study CR neurons which...

...will have achieved your goal. Yay!   You’re only human When it comes to public speaking, Murphy’s law definitely... the middle of my presentation, lost in a sea of data, my mind went completely blank. After several seconds...its most difficult form. You’re presenting complex data, to a group of peers who are there to provide critical...speak at conferences, or even just provide quick updates during a company meeting increases visibility of...

...widely popular in genome engineering. For use in human cell lines, the Zhang lab codon-optimized SpCas9...retroviral). Fun fact: published in 2003, this plasmid predates Addgene’s founding. Find pCMV-VSV-G. pRSV-Rev...

...Jonathan, et al. "Engineering of CRISPR-Cas12b for human genome editing." Nature communications 10.1 (2019... for many more specific research needs. In this update, we will focus on the most recent advances and ...

... Repair Plasmids for endogenous gene tagging in human cells Resources on Addgene.org Browse all CRISPR...FKBP12F36V fusion chimera degradation. In the cell lines we have evaluated to date, we have not observed ...Dana-Farber Cancer Institute. Targeted protein degradation In the Bradner and Gray labs, we synthesize compounds...small molecule degraders that achieve selective degradation of endogenous proteins (notably, BRD2/3/4, CDK9... Hippel-Lindau (VHL) to bring the endogenous degradation machinery into close proximity with the target...polyubiquitination of the target protein and proteasomal degradation. Remarkably, small molecule degraders provide...prolonged biological effects. While targeted degradation of endogenous proteins is an exciting strategy...

...Adeno-Associated Viral Preparations from Viral DNA Extracts. Human Gene Therapy 31:664–678 . https://doi.org/10.1089...Originally published Aug 3, 2017 and last updated Apr 6, 2021 by Will Arnold. Introducing NGS in 2017...ability to provide requesting scientists high quality data! Addgene's new in-house NGS process To start, the...seqWell we make use of a pipeline that takes the raw data from each sample in our pool and individually assembles...confirm that the species of the gene matches the data associated with the plasmid.     Confirming tags...that differs from what we would expect given the data provided by the depositing lab, we call these “quality...expected and do not affect plasmid function, we will update information on the plasmid’s page. Sometimes we...

...and then returning them to the patient. The first human trial involving CRISPR Cas9 for these diseases was...clinical therapy is more fitting, so it is time to update our post. From Mice to Men When this article was... Vertex Pharmaceuticals and CRISPR Therapeutics. Data shared from these trials has been outstanding, with...Biosciences is currently running this trial, with more updates expected in the coming months/years. The CRISPR-HIV...originally written in 2014 by Kendall Morgan and updated in 2023 by Susanna Stroik.  References and Resources...genome editing to treat sickle cell disease-A brief update. Front Med (Lausanne). 2023 Jan 9;9:1065377. doi...

...synthetic biology to create tools to better understand human disease.  Outside the lab, he is interested in communicating...meeting was designed to explore areas where further data is necessary to determine the best solutions. The...of the Federal Labor Standards Act (FLSA) which mandates that all workers across the country either be ...labor-of-love’ philosophy of academic science to date. Advocacy opportunities for the future No matter...nationwide. On this level, FoR is currently compiling a database of institutional responses to the FLSA ruling....PMC4304227. 4. Revitalizing biomedical research: recommendations from the Future of Research Chicago Symposium...