We narrowed to 266 results for: iNOS

1. Developing Lab Management Software for Biology

   Type

   Blog Post

   Published

   Aug. 25, 2016, 2:30 p.m.

   ...about how they would like these things tracked. Terminology and language are tricky Names for things will...

2. Addgene's Tips for Plasmid Quality Control

   Type

   Blog Post

   Published

   Jan. 14, 2016, 3:30 p.m.

   ... sequence from a typical reaction, or about 250 amino acids worth, so make sure the feature you want to...

3. Plasmids 101: Golden Gate Cloning

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   Blog Post

   Published

   Aug. 27, 2015, 2:30 p.m.

   ... with an attB recombination scar encoding eight amino acids, but Golden Gate assembly can be designed ...

4. Fluorescent Proteins 101: Introduction to FRET

   Type

   Blog Post

   Published

   June 27, 2017, 1:03 p.m.

   ...end positions without changing the order of the amino acids in the protein) may be able to boost FRET ...

5. Plasmids for Endogenous Gene Tagging in Human Cells

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   Blog Post

   Published

   April 6, 2017, 1:02 p.m.

   ... which terminus, N or C, to tag and which short amino acid linker to use. These decisions were made based...

6. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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   Blog Post

   Published

   May 22, 2018, 1:38 p.m.

   ...Thomas, D., & Surdin-Kerjan, Y. Metabolism of sulfur amino acids in Saccharomyces cerevisiae. Microbiol Mol...

7. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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   Blog Post

   Published

   Sept. 26, 2019, 12:50 p.m.

   ... editing efficiencies of the chimeric and fully albino mice ranging from 11% to 100%, a level similar ...

8. Mouse Modeling, Part 1: Genetically Engineered Mice

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   Blog Post

   Published

   July 11, 2019, 1:26 p.m.

   ...first blog post in this series, I’ll highlight terminology you should be familiar with before working with...

9. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

   Type

   Blog Post

   Published

   Oct. 6, 2020, 1:15 p.m.

   ...doi.org/10.1038/nmeth.3871 Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib...

10. Hot Plasmids and Viral Preps - March 2021

    Type

    Blog Post

    Published

    March 23, 2021, 1:15 p.m.

    ...fluorescent proteins (FPs) fused to the AID* (minimal 44 amino acids auxin inducible degron sequence) tagged proteins...

11. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type

    Blog Post

    Published

    March 12, 2020, 1:15 p.m.

    ...Szikra, T., Esposti, F., Cowan, C.S., Bharioke, A., Patino-Alvarez, C.P., Keleş, Ö., Kusnyerik, A., Azoulay...

12. Progress Towards a PAM-Free CRISPR

    Type

    Blog Post

    Published

    March 7, 2024, 2:39 p.m.

    ...knock-in a patient mutation or need to make a precise amino acid change, there is very little flexibility in...

13. Hot Plasmids February 2024

    Type

    Blog Post

    Published

    Feb. 20, 2024, 2:45 p.m.

    ...the epitope tags we know and love, the FLAG tag (amino acid sequence DYKDDDDK) may be one of the most versatile...

14. 6 Tips for Grant Writing

    Type

    Blog Post

    Published

    Oct. 18, 2016, 2:30 p.m.

    ...other scientists that we forget that some of the terminology we use routinely isn’t common to non-scientists...

15. Experimenting in an Open Source lab: from CRISPR to Cats

    Type

    Blog Post

    Published

    July 23, 2015, 2:30 p.m.

    ...was not possible, but one of our members took an Arduino microcontroller and a motor controller and made...

16. Hot Plasmids: Summer 2025

    Type

    Blog Post

    Published

    Aug. 28, 2025, 1:15 p.m.

    ...small enough to be carried by viral vectors (~400 amino acids, less than a third of the size of Cas9). ...

17. Viral Vectors 101: Producing Your rAAV

    Type

    Blog Post

    Published

    July 16, 2024, 1:15 p.m.

    ... https://doi.org/10.1089/hum.2010.107 Zen, Z., Espinoza, Y., Bleu, T., Sommer, J. M., & Wright, J. F. ...

18. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type

    Blog Post

    Published

    Sept. 12, 2017, 1:44 p.m.

    ...viral production. This recombination produces a minor pre-recombined population that does not require ...

19. Truncated gRNAs for Regulating Gene Expression

    Type

    Blog Post

    Published

    Jan. 10, 2017, 3:37 p.m.

    ...additional effector domains (4). Taken together, these minor gRNA alterations can be exploited to quickly and...

20. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type

    Blog Post

    Published

    March 28, 2018, 6:52 p.m.

    ...bacteria where cleavage leads to the loss of spectinomycin resistance. xCas9 3.7 showed the broadest PAM...