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We narrowed to 440 results for: pes;

Showing: 181 - 200 of 440 results

  1. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...sophisticated gene expression controls. Most cell types do not respond to low-intensity light, so off-target...systems ensures that they can be used with many types of proteins, including Cas9 and dCas9-transcription...

  2. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...Connie Cepko to learn about her management style. Recipes for delegation success Remember that there are ...authority, responsibility and accountability? What types of issues do you want to know about right away? ...

  3. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ...spanning 20 years of research. DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes.... 2015 Jan 15;29(2):197-211. DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes...

  4. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ... with all the new biotechnology.” These are the types of inquiries that routinely hit the inbox at the...ideas ranged from a biological breathalyzer to pesticide breakdown, production of fire-resistant housing...

  5. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ... creating and sharing knowledge, related to the types of materials we help distribute and the research...Then we format, do a final review to catch those pesky typos, and hit publish. Making content is an iterative...

  6. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...experiences, but the increased binding can mask the epitopes antibodies recognize. It is therefore best to ...for one that has been validated in multiple assay types. Remember that ChIP uses native proteins (so antibodies...

  7. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ... packaging, and transfer) are supplied by three types of plasmids, which are cotransfected into the 293T...flexibility to pseudotype γ-retrovirus using different envelopes to modify tropism. Briefly, different envelope ...

  8. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...relatively low retrograde transport efficiency in most serotypes. Some viruses (e.g., rabies) naturally exhibit... be transduced equally well by AAV-retrograde serotypes (Skorput, 2022, Albaugh, 2020, Tervo, 2016).  ...

  9. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...Tools blog can help you learn about the different types of viruses and pick one for your experiment. Research... of transduction in difficult-to-transfect cell types ranging from primary to embryonic stem cells. Viral...

  10. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...and gamma (Gγ). G proteins are divided into four subtypes, based on their Gα subunit: Gαs, Gαi/o, Gαq/11...GPCRs show a propensity to associate with certain subtypes, although most are able to signal through more...

  11. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...expressing plasmid. Envelope proteins determine which types of cells a virus can infect. The VSV-G envelope ...enables this virus to infect many different cell types so it is useful in a wide range of applications....

  13. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...member. But, at Addgene, we use seven different types of “plates” (Fig. 1). When you have to tell an engineer... barcoded (this is pretty Addgene specific) The types or purposes of each given container, when applicable...

  14. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...amount of working memory resources. There are three types of cognitive load: intrinsic cognitive load is the...organizing and connecting information and a logic to the types and timing of questions that are asked. Many things...

  15. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...antibodies are antibodies generated against antibody isotypes from a specific species, such as the rabbit IgG...primary antibodies are different species and/or isotypes. For highly multiplexed assays, using secondary...

  16. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...multiplexing and can be used in a variety of assay types. Be sure to check the spectral properties of the... et al., 2007; Stoeckius, et al., 2017). Metal isotopes are useful for mass cytometry (Bendall, et al....

  19. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...connected to these 3 stages and contains multiple cell types: fibroblasts, myofibroblasts, neuroendocrine, adipose...drug resistance? Although we’ve seen how multiple types of cells affect tumor progression, enzymes and metabolism...

  20. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...embryonic stem cells, and/or tissue-specific cell types: Cre-lox recombination. What is Cre-lox? The Cre-lox... that are active only in certain cell or tissue types, during certain stages of development, or by making...
Showing: 181 - 200 of 440 results