We narrowed to 188 results for: pme i
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TypeBlog PostPublishedMarch 6, 2018, 1:49 p.m....PMC516055. 23. Pascal, John M., et al. "Human DNA ligase I completely encircles and partially unwinds nicked ...conditions, and formulations and has led to the development of the new HiFi Taq DNA Ligase. Using this method... PMID: 23142428. 17. Hamada, Mariko, et al. "Development of a ligase detection reaction/CGE method using...PMID: 23463388. 18. Hommatsu, Manami, et al. "Development of a PCR/LDR/flow-through hybridization assay...
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Technologies Enabled by NanoLuc® Luciferase
TypeBlog PostPublishedFeb. 8, 2018, 12:17 p.m....ever since there were plasmids to share. Back when I was in the lab, if you read a paper and saw an interesting...Hooper, PhD, has also worked with R&D for product development and now specializes in supporting Promega cellular... -
PiggyBac-ing Through the Genome Editing Field
TypeBlog PostPublishedMay 31, 2016, 3:30 p.m....T. Dargitz, R. Wright, A. Khanna, F. H. Gage, and I. M. Verma, “Functional Gene Correction for Cystic ...efficient manner than ever before, thanks to the development of site-specific nucleases (CRISPR/Cas9, TALENs... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog PostPublishedJune 1, 2023, 1:15 p.m....gang, Mesik, L., Liang, F., Tao, H. W., & Zhang, L. I. (2017). AAV-Mediated Anterograde Transsynaptic Tagging...vivo. Molecular Therapy - Methods & Clinical Development, 3, 16062. https://doi.org/10.1038/mtm.2016.62... -
Antibodies 101: Introduction to Antibodies
TypeBlog PostPublishedJan. 19, 2021, 2:15 p.m....that antibodies can be used for a lot of purposes! I hope you appreciate how many types of antibodies are...reagent sharing platform for antibodies, the Developmental Studies Hybridoma Bank Learn more about nanobodies... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog PostPublishedJune 28, 2018, 3:01 p.m....crRNAs that bind to the template strand. Therefore, I suggest that picking a crRNA complementary to the ...manipulation of mammalian neural stem cells. Development 144: 635-648. PubMed PMID: 28096221. PubMed Central... -
Optogenetics + CRISPR, Using Light to Control Genome Editing
TypeBlog PostPublishedSept. 3, 2020, 12:15 p.m..../doi.org/10.1038/nchembio.1753 Richter F, Fonfara I, Bouazza B, Schumacher CH, Bratovič M, Charpentier...Dalvie NS, Panda A, Fleming HE, Bhatia SN (2016) Development of Light-Activated CRISPR Using Guide RNAs with... -
Technical Design of a Western Blot
TypeBlog PostPublishedAug. 22, 2024, 1:15 p.m....., Podrebarac, D. M., Greenough, W. T., & Weiler, I. J. (2008). The use of total protein stains as loading...that each type of transfer requires different equipment. Wet transfers can be done in an insert in your...