...from a replicating adenovirus using native viral promoters. Mol Ther, 7(4), 526–534. https:/doi.org/10.1016...as other intracellularly-exposed proteins and downstream cellular events, all recruit strong innate and...

...help them navigate the scientific enterprise and promote their voices in driving changes in science. Follow...that salaries may even double. The University of Notre Dame sets no minimum, stating that while they recommend...

... in mapping of regulatory elements (to mutate promoters/enhancers in reporter constructs), in functional...introduced anywhere in the plasmid (albeit at extremely low frequency), and these could interfere with...

...When you go on interviews or are looking for a promotion, have some solid examples of times when you have...natural inclination for this skill that you could strengthen. The right set of transferable skills, coupled...other just for the sake of disagreeing. It can be extremely toxic in a lab or work environment if left unchecked...conflict resolution is likely not one of your strengths. Empathizing with people, especially people with...conflicts within a given organization, it can help tremendously to have as much context as possible. When I ...recognize that conflict resolution may never be a strength for you. But if you are a problem solver, and ...

...RNA (protospacer+scaffold) in a pol-III (OsU3) promoter driven expression system. We use a single polycistronic...conversion of a target ‘A’ to ‘I’ (inosine), which is treated as ‘G’ by cellular polymerases. Subsequently, an...developed using the hAPOBEC3 deaminase can edit in a stretched window ranging from 1-17 bp relative to the PAM...

...that binds specifically to phosphate moieties and promotes a robust mobility shift in phosphorylated, but...pSer-incorporation on enzyme function through phosphatase treatment. If a candidate phosphatase is unknown, CIP or...

...nuclease-dead Cas9 (dCas9) is directed near the promoter of a target gene. Here, the target window is not...window upstream of the transcription start site (TSS), while for CRISPRi, a ~100nt window downstream of the...ability of the cell to use an alternative ATG downstream of the annotated start codon. Likewise, we avoid...

...sites on the target DNA, and thus inactivating the promoter. Image reused with permission from Neumann et ...this proof-of-concept study) are captured on streptavidin-coated magnetic beads through an assembly of...with a GFP nanobody (Figure 1). This enrichment streamlines the purification and minimizes sample loss while...

...integration at regions of open chromatin, especially at promoter or exonic regions. If the cell’s chromatin structure...piggyBac-ing models and new therapeutic strategies.,” Trends Biotechnol., vol. 33, no. 9, pp. 525–33, Sep. 2015...

...expression from rAAVs also depends on your choice of promoter/enhancer. Table 1: AAV capsids and receptors. ...developed from prior natural exposure or rAAV treatment, which can pose problems for gene therapy applications...therapeutic outer retinal gene delivery from the vitreous. Science Translational Medicine, 5(189), 189ra76...

...example, piggyBac and Tol2 are best for screening promoters and enhancers since they are biased to insert ...from the donor molecule, and inserts it ~50 bp downstream of QCascade’s genomic target site (Fig. 3B). ...from the donor molecule, and inserts it ~50 bp downstream of QCascade’s genomic target site.  T-RL= transposon...

...recombinant adeno-associated virus (scAAV) vectors promote efficient transduction independently of DNA synthesis...of AAVs in humans AAVs are already being used to treat disease in humans and additional improvements are...transduction efficiency, and safety profiles should strengthen its appeal for the scientific and medical communities...

...activates hypoxia inducing factor (HIF-1), which promotes the production of VEGF to increase angiogenesis...labor with up and coming immunotherapy treatments. As treatments enter the clinic, physicians and researchers...more vasculature to feed themselves, use the blood stream to commute to and invade multiple organs, and most...cause, manifestation, diagnosis, recurrence, and treatment of cancer have been extensively explored. Biologists...compromised either due to age, infections, or other strenuous biological changes. In contrast to the acute immune... cytokines like TGFβ and IL-6. However, when strengthened through efforts to boost the activity of the... ability of even these conventional antitumor treatment strategies can be enhanced. Types of immunotherapeutics...

...spectrum. When photons are absorbed by matter, they promote electrons within atoms to a higher energy state...fluorescence. Another way to label proteins is through treating cells with fluorophore-conjugated antibodies that... fluorophore's brightness refers to the signal strength of the emitted photons detected by the cytometer...

...iPSC-derived osteoblasts and osteoclasts together promote bone regeneration in 3D biomaterials. Sci Rep, ...examples of transdifferentiation in vitro where treatment of mouse fibroblasts with 5-azacytidine led to...Mukherjee. Kusumika (Kushi) Mukherjee is the Editor of Trends in Pharmacological Sciences, a Cell Press reviews...adult pancreatic alpha-cells to beta-cells after extreme beta-cell loss. Nature, 2010. 464(7292): p. 1149...expression of insulin genes in liver and ameliorates streptozotocin-induced hyperglycemia. Nat Med, 2000. 6(5):... PMID: 18754011. 43. Zhou, Q. and D.A. Melton, Extreme makeover: converting one cell into another. Cell...

...because it can process multiple gRNAs under a single promoter, allowing for easy multiplexing. The double-stranded...bacterial and archaeal immune systems has led to extreme sequence divergence that requires a multi-pronged...subtypes, A–C, with the beloved SpCas9 (from Streptococcus pyogenes) belonging in II-A. Cas9 was the first... CRISPR-Cas: beyond the Cas10 effector complex. Trends in Biochemical Sciences, 49(1), 28–37. https://...