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We narrowed to 205 results for: tre promoter

Showing: 181 - 200 of 205 results

  1. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ...skills guide: managing a team. You’ve just been promoted, congratulations! You’re now a manager with your...motivates you!), know their unique skills and strengths (you will likely have reports with different levels...

  2. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...while also broadening access to experiences that promote professional success (many of my students include...1977; Ma et al., 2020). Many have interpreted this trend as evidence that the best teachers and mentors pass...

  3. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...be these skills that get you your next job or promotion. Also, don’t wait until you’re out of the lab ...question today, because I sometimes wish for a stretch of time that doesn’t involve critical thinking....

  4. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...others understand ours. Improving access to data promotes dissemination, especially for young researchers...typically consortia involving genomic screens, the data stream is so prolific that raw information is made available...

  5. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    Published
    July 29, 2025, 1:15 p.m.
    ... difference between them lies in the choice of promoter for ωRNA (reRNA) expression (see Figure 1). Both... PAM sites. For Cas9, the PAM is located just downstream (3′ end) of the target sequence, while for Cas12a...Cas12a and TnpB, the motif is found upstream (5′ end) of the target site. Additionally, the TAM changes... region binds to the DNA sequence immediately downstream of the TAM. This RNA-DNA hybrid formation recruits...cleaves the DNA at a position 15–21 base pairs downstream of the TAM, creating staggered double-strand ...target gene. Choose a 20-nt sequence immediately downstream of the TAM for targeting. While plant-specific...

  6. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...enough to prevent confluency but high enough to promote cell growth. Overgrown cultures stimulate quiescence... yeast, fungi, or bacteria, mycoplasma can be extremely hard to detect with levels reaching 108 cells ...Daily medium exchanges help alleviate cellular stresses associated with pH changes and resource consumption...particles you harvest. Studies have demonstrated that extreme changes in osmotic pressure and pH can destabilize...labs try to use freshly collected lentivirus for downstream applications. While this ensures that the highest...23179822. 5. Segura, M.M., Kamen, A., Garnier, A. “Downstream processing of oncoretroviral and lentiviral gene...

  7. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...beneficial for many systems. How the EDGE Program promotes scientific sharing The EDGE Program is designed...to study life’s innovations from anywhere in the tree of life.” Accepted proposals were required to present... arose in different branches of the evolutionary tree at least 6-7 times and on at least 2 separate continents...especially cardenolides which have been used to treat heart disease. Caterpillars that eat milkweed generate...Joshua Rosenthal) The Hawaiian bobtail squid has an extreme number of nerve cells and an unusually high level...

  8. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...interest. A low primer-to-template ratio is used to promote annealing of one primer to each template. The PCR...restriction site, but nick opposite strands of DNA. Treating the plasmid with ExoIII and ExoI then fully degrades...by Wrenbeck et al but it’s likely that longer stretches of sequence could be used. However, a practical...assessing protein function on a massive scale." Trends in Biotechnology 29.9 (2011): 435-42. PubMed PMID...

  9. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...from a replicating adenovirus using native viral promoters. Mol Ther, 7(4), 526–534. https:/doi.org/10.1016...as other intracellularly-exposed proteins and downstream cellular events, all recruit strong innate and...

  10. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ...help them navigate the scientific enterprise and promote their voices in driving changes in science. Follow...that salaries may even double. The University of Notre Dame sets no minimum, stating that while they recommend...

  11. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ... in mapping of regulatory elements (to mutate promoters/enhancers in reporter constructs), in functional...introduced anywhere in the plasmid (albeit at extremely low frequency), and these could interfere with...

  12. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...When you go on interviews or are looking for a promotion, have some solid examples of times when you have...natural inclination for this skill that you could strengthen. The right set of transferable skills, coupled...other just for the sake of disagreeing. It can be extremely toxic in a lab or work environment if left unchecked...conflict resolution is likely not one of your strengths. Empathizing with people, especially people with...conflicts within a given organization, it can help tremendously to have as much context as possible. When I ...recognize that conflict resolution may never be a strength for you. But if you are a problem solver, and ...

  13. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...RNA (protospacer+scaffold) in a pol-III (OsU3) promoter driven expression system. We use a single polycistronic...conversion of a target ‘A’ to ‘I’ (inosine), which is treated as ‘G’ by cellular polymerases. Subsequently, an...developed using the hAPOBEC3 deaminase can edit in a stretched window ranging from 1-17 bp relative to the PAM...

  14. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...that binds specifically to phosphate moieties and promotes a robust mobility shift in phosphorylated, but...pSer-incorporation on enzyme function through phosphatase treatment. If a candidate phosphatase is unknown, CIP or...

  15. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...nuclease-dead Cas9 (dCas9) is directed near the promoter of a target gene. Here, the target window is not...window upstream of the transcription start site (TSS), while for CRISPRi, a ~100nt window downstream of the...ability of the cell to use an alternative ATG downstream of the annotated start codon. Likewise, we avoid...

  16. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    Published
    Oct. 2, 2025, 1:15 p.m.
    ...HiBiT is compatible with both strong and weak promoters, and even modest endogenous pools generate high...protein. Together, the work links p53 mutations to treatment outcomes and provides a HiBiT-enabled framework...track the internalization of EGFR in real time treatment with EGF. Due to the lack of membrane permeability... HiBiT still present on the cell surface. EGF treatment resulted in rapid internalization and diminished...

  17. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...sites on the target DNA, and thus inactivating the promoter. Image reused with permission from Neumann et ...this proof-of-concept study) are captured on streptavidin-coated magnetic beads through an assembly of...with a GFP nanobody (Figure 1). This enrichment streamlines the purification and minimizes sample loss while...

  18. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...integration at regions of open chromatin, especially at promoter or exonic regions. If the cell’s chromatin structure...piggyBac-ing models and new therapeutic strategies.,” Trends Biotechnol., vol. 33, no. 9, pp. 525–33, Sep. 2015...

  19. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...example, piggyBac and Tol2 are best for screening promoters and enhancers since they are biased to insert ...from the donor molecule, and inserts it ~50 bp downstream of QCascade’s genomic target site (Fig. 3B). ...from the donor molecule, and inserts it ~50 bp downstream of QCascade’s genomic target site.  T-RL= transposon...

  20. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...recombinant adeno-associated virus (scAAV) vectors promote efficient transduction independently of DNA synthesis...of AAVs in humans AAVs are already being used to treat disease in humans and additional improvements are...transduction efficiency, and safety profiles should strengthen its appeal for the scientific and medical communities...
Showing: 181 - 200 of 205 results