We narrowed to 465 results for: val

...Appropriate fragment and primer design results in covalently joined, scarless DNA – perfect for transforma...Addgene.org Browse Our CRISPR Resources Find Validated gRNAs Browse All Plasmids ...

...engineering Escherichia coli to see light. Levskaya A, Chevalier AA, Tabor JJ, Simpson ZB, Lavery LA, Levy M, Davidson...detection program. Tabor JJ, Salis HM, Simpson ZB, Chevalier AA, Levskaya A, Marcotte EM, Voigt CA, Ellington...

...is contrary to reverse screens, where there is removal of known factors (such as a genes) and the loss...cellular pathway of interest conveys no significant survival advantage, such as a non-essential mechanism or...

...their work. Then, I distill and translate that valuable information back to the rest of my team in Oxford...which you’re building software will help you be a valuable asset to the scientists as well as the developers...

...reduce the risk of plasmid recombination. How do I validate my ITR sequences?  Next-generation sequencing ... the enzymes SmaI and SrfI can also be used to validate ITR sequences as these enzymes cut at known sites...

...etc.). The transducer will generate a numerical value which represents the amount of analyte present in...levels and converted that signal into a readable value. Types of biosensors So now that we know what makes...

...project. New England Biolabs and Alnylam see the value to the scientific community in accelerating the ...lab from Janelia Research Campus have provided valuable insight into AAV production. Finally, we are ...

...consistency and validation with >80% effectively enriched guides per gene hit, and 100% validation of the top...

... Can be used for color and growth selection if divalent lead ions are used in the growth media. ura4...commonly employed, with the KanMX being the most prevalent due to its versatility and ability to be used ...

...advocates have argued that while peer review is valuable, modern scientific publishing has a number of ...Burgess SJ, Schwessinger B, Hensel Z (2019) On the value of preprints: An early career researcher perspective...

...optogenetics and chemogenetics to study the activity of parvalbumin (PV)-expressing neurons, neurons that the lab...work on the PV neurons is in progress, she has validated the dual virus method to study CR neurons which...

...hear it all the time, but Addgene is a totally invaluable resource. I really appreciate the work you do...electronic signatures to dramatically speed up MTA approvals. In 2013 we were thrilled to receive the Massachusetts...

...do a head-to-head comparison right, it will be valuable information for the scientific community. The ...comparison by others. Together, we can build a valuable resource with tools and data that indicate the...

...gene expression from stabilized promoters were equivalent whether the genes were expressed from the genome...microarray. But, aside from facilitating the joys of a p-value < 0.05, having such fine control of gene expression...

...and their upregulation is correlated with poor survival in breast cancer patients. By digging into the...Zarnegar, B.J., Mondal, S., Roth, J.G., Gai, H., Kovalski, J.R., Siprashvili, Z., Palmer, T.D., Carette,...

...and going frequently - an environment ripe for valuable work and materials to slip through the cracks....working on interesting biotechnology code.There are valid technical reasons to choose a non-Python stack, ...

...perfect, so even though it is science’s way of validating work prior to publication, it doesn’t mean that...After all, the science was performed, written and evaluated by humans. Keeping up with the literature There...

... exactly what antibody they are using and any validation experiments performed will be useful in perpetuity...518027a Bradbury AM, Plückthun A (2015) Antibodies: validate recombinants once. Nature 520:295–295 . https:...

... can result in the retention of introns or the removal of exons. Disrupting promoter or transcription ...) deletion. (B) Mutations of splice sites. (C) Removal of an exon or a large genomic fragment. (D) Knock-in...

...Additionally, controls available in the system are validated and were chosen because of their broad applicability...process down into four easy steps, provides the real value for the user. Let's walk through designing a CRISPR...