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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 456 results for: val

Showing: 181 - 200 of 456 results

  1. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...project. New England Biolabs and Alnylam see the value to the scientific community in accelerating the ...lab from Janelia Research Campus have provided valuable insight into AAV production. Finally, we are ...

  2. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...etc.). The transducer will generate a numerical value which represents the amount of analyte present in...levels and converted that signal into a readable value. Types of biosensors So now that we know what makes...

  3. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...is contrary to reverse screens, where there is removal of known factors (such as a genes) and the loss...cellular pathway of interest conveys no significant survival advantage, such as a non-essential mechanism or...

  5. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ... Can be used for color and growth selection if divalent lead ions are used in the growth media. ura4...commonly employed, with the KanMX being the most prevalent due to its versatility and ability to be used ...

  6. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...and their upregulation is correlated with poor survival in breast cancer patients. By digging into the...Zarnegar, B.J., Mondal, S., Roth, J.G., Gai, H., Kovalski, J.R., Siprashvili, Z., Palmer, T.D., Carette,...

  7. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...do a head-to-head comparison right, it will be valuable information for the scientific community. The ...comparison by others. Together, we can build a valuable resource with tools and data that indicate the...

  8. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...advocates have argued that while peer review is valuable, modern scientific publishing has a number of ...Burgess SJ, Schwessinger B, Hensel Z (2019) On the value of preprints: An early career researcher perspective...

  10. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...hear it all the time, but Addgene is a totally invaluable resource. I really appreciate the work you do...electronic signatures to dramatically speed up MTA approvals. In 2013 we were thrilled to receive the Massachusetts...

  12. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ...and going frequently - an environment ripe for valuable work and materials to slip through the cracks....working on interesting biotechnology code.There are valid technical reasons to choose a non-Python stack, ...

  14. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ...internal control (ATCC#VR-1516) until the assay is validated. Infectious titer: (Plaque Forming Units (PFU...Nanette, Keith L. March, and Bruce C. Trapnell. "Evaluation of the concentration and bioactivity of adenovirus...

  16. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ... can result in the retention of introns or the removal of exons. Disrupting promoter or transcription ...) deletion. (B) Mutations of splice sites. (C) Removal of an exon or a large genomic fragment. (D) Knock-in...

  17. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...Additionally, controls available in the system are validated and were chosen because of their broad applicability...process down into four easy steps, provides the real value for the user. Let's walk through designing a CRISPR...

  18. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...antibody options for your experiment (all well validated for your application, of course!) When the stars...these. After all, there’s no sense in spending valuable research dollars on a new secondary antibody if...

  19. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ... off-target binding. The activity of SpRY was validated in double stranded cleavage and base editing applications... Flexibility History of CRISPR Cas – A Tale of Survival and Evolution  ...

  20. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...details. Not only will these mStayGold variants be valuable tools for your next experiments, they will also...expression than AAV9. This new capsid will be a valuable tool for investigating NHP brains. Figure...
Showing: 181 - 200 of 456 results