We narrowed to 783 results for: INA

... one target is labeled and imaged at a time, inactivating the fluorescent secondary antibodies before ...comes in. After you image the first target, you inactivate the fluorophores in the sample, either by chemical...new primary, wash, add secondary, image again, inactivate, and repeat these cycles for all your targets...from. But by labeling sequentially with one, inactivating the fluorophores, then labeling with the other...brain samples from the James Trimmer Lab, using recombinant antibodies with different isotypes. (A) Neocortex...antibodies are commercially available. Pick whichever combination of host and target species, isotype specificity... 2A above, Andrews et al. used the following combination of antibodies:   Target (Rat) Primary antibody...

...piggyBac™ transposon? The piggyBac™ transposon was originally identified in moths in 1983, but wasn’t successfully...or when the gene is constitutively-active or a dominant-negative mutant. Reducing the copy number can ...host machinery for DNA repair by homologous recombination, which results in the incorporation of your ...containing a negative selection marker such as thymidine kinase, a researcher may subsequently kill off any cells...site-specific nuclease to stimulate homologous recombination and Footprint-Free™ removal of the selection...First, instead of using piggyBac™, you could use recombinase technology (Cre/loxP) to remove the donor cassette...[7], it is generally not as efficient as Cre recombinase. However, the use of negative selection largely...

...another biological workhorse, Cre recombinase, which induces recombination at LoxP sites. Placing a lox-STOP-lox...researchers to activate transcription or Cre recombinase activity only in the presence of GFP. These systems... transcription. Thus, T-DDOGs can be used in combination with green-red Cre-lox systems. T-DDOGs can also...T-DDOGs, GFP, and a reporter construct into the mouse retina. GFP expression robustly activated the reporter...Cre-DOG is both robust and specific. When tested in retinal electroporation studies, DsRed reporter expression...system, including the motor cortex, cerebellum, and spinal cord. Cre-DOG is also suitable for optogenetic ...type-specific manipulation with GFP-dependent Cre recombinase.” Nat. Neurosci. 18(9) (2015): 1334-1341. PubMed...

...novel way. Finally, engineering new applications to solve problems will provide originality to your work...on how to submit your work either through seminars or webinars. Sending on your grant application to your... the deadline isn’t going to be much use. 2. Originality is essential for grant success The two most important...be looking for in your research proposal are originality and relevance. When reading through your proposal...our own or other groups, but your work must be original (otherwise why would you be interested in it?)...Frontier Science Program's website. One way to add originality to your work is to collaborate with specialists...your work, or even the successful use of your preliminary data to secure further funding. Make it clear...

... that exterminate entire humanity were the two most popular topics that sparked the imagination of most... CBE but use a ssDNA adenosine deaminase (evolved from a tRNA deaminase enzyme, TadA) to induce a A→G ...hybrids. This book is published 30 years after the seminal work of Gregor Mendel on the genetic inheritance... first ever X-ray diffracted image of the DNA. Finally, in 1953, Franklin along with James Watson and ...Thurman and Jude Law. The movie’s name is itself a combination of the four bases of DNA – A, T, G, and C. In...These breaks can then be repaired by homologous recombination with a repair template.  Each zinc finger recognizes...The cytidine base editors (CBE) use a cytidine deaminase to introduce a C→T change at precise locations...

...communities. Many thanks to our guest blogger Katrina Armstrong. Katrina Armstrong is a Neurophysiology Msc Student...This post was contributed by guest blogger Katrina Armstrong, a Neurophysiology Msc Student at the University...which cell type? Location (3): Traveling from the original location - Axonal Transport in the CNS What tissue... Photoreceptor Cells AAV2, AAV5, AAV8 RPE (Retinal Pigment Epithelium) AAV1, AAV2, AAV4, AAV5, AAV8...your particular experiment! Traveling from the Original Location - Axonal Transport It has been demonstrated...serotonin in movement. Follow her on twitter @katrinaboards.   References 1. Russell, David W. "AAV vectors...and Richard J. Samulski. "Self-complementary recombinant adeno-associated virus (scAAV) vectors promote...

...through the use of protein tags and separated from contaminants using a variety of purification methods. Deciding...expression systems are very powerful for high level recombinant protein expression. These systems enable high...cheap and low-tech means of mass expression of recombinant proteins. Many cells from various types of plants...modifications. Extraction and purification of recombinant proteins from plants however can be expensive...biochemicals and proteins through plant roots. Tagging recombinant proteins with a naturally secreted plant peptide...expression system to generate large quantities of recombinant eukaryotic proteins. Although many species of...using S. cerevisiae, researchers often place recombinant proteins under the control of the galactose inducible...

...to the final topic, which is that natural AdVs, replication-competent viruses with a recombinant genome... Virus type 2 (AAV2) – or some unprecedented combination of the three (Servellita et al., 2023). Normally...lack of exposure, a lack of immunity, and then finally a synchronized exposure under these conditions ...virus, we will see that they are very central determinants of how AdVs behave, and in fact, of how adenoviral...from the genomic plasmid can be used to house a recombinant sequence (transgene) instead.    Although the...strong innate and adaptive immune responses that inactivate viral genomes or otherwise clear the virus from...been reported when using vectors, and immune inactivation of the vector can still occur even in the case...

...infect. Finally, the transfer plasmid encoded the desired transgene flanked by HIV-1 long terminal repeats... the original system, the possibility of creating a replication-competent virus via recombination between...encoding Gag and Pol, increasing the number of recombination events necessary to create a replication-competent...replication-competent virus. Second, Tat is eliminated through the addition of a chimeric 5’ LTR containing a Tat-independent...oncogenesis by altering local gene expression. Self-inactivating (SIN) lentiviral vectors contain a deletion ...F, Beuzard Y, Gluckman E, Bushman F, Hacein-Bey-Abina S, Leboulch P. Nature. 2010 Sep 16;467(7313):318...PubMed PMID: 25804479. 9. Development of a self-inactivating lentivirus vector. Miyoshi H, Blömer U, Takahashi...

...contrast, fluorescence imaging has emerged as the dominant light microscopy contrast technique in modern ...molecular or structural specificity to the viewer. Finally, modern microscope designs may further exploit ... light exits the microscope objective evenly illuminating the entire (wide) field of view. Fluorescent...and focused onto a camera for visualization. Illuminating a sample in the opposite direction to fluorescence...fluorescence collection is referred to as “Epi” illumination. Therefore these are sometimes call Epi-fluorescence...sample and raster scanning the point to build up a final image pixel by pixel. The collected fluorescent ...location in the sample. As more than one hole is illuminated at a time, the image can be acquired more quickly...