...yourself needing to brainstorm with a team. One major challenge with these brainstorming sessions is avoiding...with unlimited time? Another strategy for your brainstorm session is to start with something completely... ideas that I have found helpful in my team brainstorming sessions.  Allow the group time to think about...position or the longest tenure, but don’t let that stop you from speaking up.  If your ideas are not well...

...papers or are brainstorming new technological innovations. Let’s take a look at three historical techniques...that save both time and money. Yet, knowledge of historical techniques comes in handy whether you are perusing...throughout the world. Let us know your favorite "Historical Lab Technique" in the comments section below!...Benjamin Chain. "The sequence of sequencers: The history of sequencing DNA." Genomics (2015). PubMed PMID...

...Stochastic multicolor labeling is a popular technique in neuroscience and developmental biology. This... persistently fluorescent during development. Stochastic multicolor labeling systems, many based on Brainbow...for improving the level of detail possible for stochastic multicolor labeling of the brain. Labeling Drosophila...express the same XFP transgene in an adult fly. As a stop cassette is located directly upstream of the fluorescent... researchers who developed Tetbow. Conclusion Stochastic multicolor cell labeling systems allow single...

...situ hybridization histochemistry: use for mRNA detection.” The Journal of Histochemistry and Cytochemistry...interaction has also been adapted for use in immunohistochemistry and antibody labelling. In these experiments...referred to as the Avidin-Biotin Complex (ABC) immunohistochemistry (IHC). For more information on ABC and IHC...Cytochemistry : Official Journal of the Histochemistry Society, 41.12 (1993): 1751–1754.PubMed PMID: 8245422. Additional...

...proteins from E. coli, Bacillus subtilis, or Pichia pastoris depending upon your specific protein folding needs...alleles in microbial populations through time, Christopher Marx's lab has engineered and validated a new...one-pot reaction. Using this system, you can create custom RNA binding proteins in as little as 3 days. Abil...Abil et al., 2014 show that you can fuse custom PUF proteins to a translational repressor to down-regulate...Rajarshi, et al. "Engineering RNA endonucleases with customized sequence specificities." Nature communications...

...Science History Rap Battle A great example of science story telling following the unfortunate story of Rosalind...and their importance in understanding biological history. 4. Please Don't Kill My Hive Beautifully filmed...

...the research community. Customers have taken advantage of the customizability, choosing the cargo, serotype... ship them out in record time.  However, our in-stock preps remain a popular choice thanks to the convenience...) Looking for more viral vectors? Browse our in-stock viral vectors or consider our AAV Packaged on Request...

...Location, Location, Location! Failure to Plan (for Storage) Is Planning to Fail Patience Is Bitter but Its... the transport issues. 2. Failure to plan (for storage) Is planning to fail Freeze/Thaw Cycles Of course...cycles can still reduce AAV titer. For long term storage, make > 25 μl aliquots in 0.5 ml tubes, snap freeze... liquid nitrogen or a dry ice/ethanol bath, and store at -80° C. Once thawed, the AAV aliquot should be... PubMed Central PMCID: PMC3098627. 6. Salegio, Ernesto A., et al. "Axonal transport of adeno-associated...

...documenting your plasmids, storing them, providing quality control and customer support, making plasmids...Yesterday we hit a huge milestone here at Addgene – 500,000 plasmids shipped! That's a 1/2 million plasmids...The trust of our depositors to allow us to help store and distribute their plasmids. The partnerships ...

...the CRISPR types and is thought to be the common ancestor of all other CRISPR systems. Type III systems ...(like me!) of CRISPR types — weird and poorly understood. There are three subtypes, A–C, and they are ...mechanism of type II is, unsurprisingly, well understood, and the first taught when introducing CRISPR...Covarrubias, P. C., Shmakov, S. A., Silva, C., Tolstoy, I., Johnson, D. B., Koonin, E. V., & Quatrini,...., Hunnewell, P., Alfonse, L. E., Carte, J. M., Keston-Smith, E., Sothiselvam, S., Garrity, A. J., Chong...