We narrowed to 535 results for: car

... PITCh plasmic is cotransfected with a plasmid carrying Cas9, the PITCh-gRNA, and the locus-specifc gRNA... Contransfect the PITCh vector with the vector carrying Cas9 and the PITCh- and locus-specific gRNAs Step...

...this protein compartment was used to optimize D-glucaric acid biosynthesis. This MPyV system encapsulated...with this encapsulated enzyme produced 20% more D-glucaric acid, suggesting that MPyV system can improve ...

...than AAV vectors. This capacity is large enough to carry the Cas9 gene and a gRNA expression cassette in ...of AdV-modified human cells being subjected to scarless homology-directed genome editing (Maggio et al...

... exonuclease, a polymerase, and a DNA ligase to carry out a pooled set of ligations in an hour or less...one to simultaneously complete an ordered set of scarless ligations following digestion with Type II restriction...

...Sharpie to circle 12-48 individual colonies by (carefully) holding plates up to the light, where they appear...diluted from 10X and containing both Hepes and bicarbonate) is brought to room temperature and placed in...

...pU6cm promoter, viral replicon amplification of cargo, and heat treatment.  Figure 1: Left: recombined...and adopts an open conformation in the absence of cargo. bioRxiv 2024.12.20.629623; doi: https://doi.org...

...can be transfected with your transfer plasmid, carrying your gene of interest, and the two helper plasmids...undergoing titering, to remove residual plasmid DNA carried over from the production process. For more details...

...15%, 25%, 40%, and 60% iodixanol solutions are carefully layered and then the viral suspension generated...digested to remove any residual AAV plasmid that was carried over from the AAV production process. Either SYBR...

...of engineered Type IIS restriction enzymes and careful choice of junction sequences guided by experimentally-derived...of overhangs can lead to incorrect assemblies, so care must be taken to minimize this possibility. Recently...

...high-throughput experiments. If a user wants to carry out a high-throughput genetic screen, it is expensive...marker can be removed by Cre-mediated excision for scarless tag insertion.   In addition to the obvious advantages...

...expect to see for the unknowns in your experiment. Carefully set up a standard curve dilution series that takes...increase the accuracy of your experiment.  Finally, carefully analyze the data and use the trendline that's ...

...numbers for an event you are planning, dangle the carrot (or more popularly- cookies). 3. Do something ...other posts about life at Addgene!Read more of our careers posts!    ...

...translated protein. As depicted in the accompanying cartoon, they have a multitude of uses including (but not...handbooks for protein purification is provided by GE Healthcare Life Sciences. Addgene pages and blog posts ...

...antibiotics is certainly a topic of great concern in healthcare today, but it doesn’t have to be some looming...resistance from the CDC. Antibiotic resistance can be scary to think about, but we can all do our part to learn...

...problem-solving process?  You may want to start by carving out time to tackle it. It’s easy to get distracted...been building your creativity all along. In my own career, my team has used these techniques to optimize ...

...Overview An immunoprecipitation reaction is typically carried out in one of two ways. Both methods utilize the...approach appealing, but the disadvantages must be carefully considered before using. For example, the tag ...

... Figure 4: Reporters in Gene On include a mScarlet reporter, luciferase reporter, and neomycin resistance...translation of an array of different reporters including mScarlet-I, luciferase, antibiotic resistance markers, ...

...conferences should strive to provide support to early career scientists, students, and those from historically...build and expand their networks and further their career development, and the scientific community can improve...

...invaluable reagents with the scientific community. Note: Cary Valley and Mary Gearing contributed to the update...PMC4660269. 7. Joung J, Ladha A, et al., ‘Point-of-care testing for COVID-19 using SHERLOCK diagnostics....

...line is maintained for ~20 passages before being discarded and replaced with a new vial of early passage ...produced in the line will be taken offline and discarded. Confirmation of the transfer plasmid Addgene ...

... in cold conditions and preferably on dry ice. Careful use of your glycerol stocks When your lab adopts...experiments. The term ‘insert’ refers to plasmids carrying each part, ‘vector’ refers to acceptor plasmids...

...promoter on the plasmid. Many commercial strains carry the DE3 lysogen, as indicated by the name of the...reasonable time-frame. For these cases, some strains carry an additional measure of control such as the pLys...

...chromosomal DNA. Chromosomal replication in bacteria is carefully regulated to ensure that it occurs at the appropriate...DNA replication in bacteria, so the process is carefully regulated by a mix of positive and negative regulators...

...This post was originally written by Maria Soriano-Carot in September 2016 and was updated by Rachel Leeson... career...

... the cell type of interest (Atasoy et al. 2008; Cardin et al. 2009). Ligand regulated recombinase - keeping...biotechnology 16(7): 657–62. PubMed PMID: 9661200.  3. Cardin, Jessica A. et al. 2009. “Driving Fast-Spiking ...

...First, CRISPR would need to be delivered to both cardiac and skeletal muscle cells, where precision editing... each observed the same positive phenotypes in cardiac and skeletal muscle. Even when the genome editing...

...coupled with your technical skills, can open new career opportunities for you, if you can identify them...eye out for the phrase “I assumed they were taking care of that”, because that can indicate confusion around...

...available resources and give yourself time to carefully review your options. If possible, try to select...fluorescence-microscopy/   Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, ...

...Equipment needed: Mini PCR machines by DIY-Bio, P51 cardboard fluorescence visualizer with 9V battery Approach...fluorescently labelled RNA, the authors employ a P51 cardboard fluorescence visualizer that is powered by a 9V...

...Learn about Careers in Science Communication Resources at Addgene.org Check out Careers at Addgene ...