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Showing: 301 - 330 of 523 results

  1. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ... PITCh plasmic is cotransfected with a plasmid carrying Cas9, the PITCh-gRNA, and the locus-specifc gRNA... Contransfect the PITCh vector with the vector carrying Cas9 and the PITCh- and locus-specific gRNAs Step...

  2. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...expect to see for the unknowns in your experiment. Carefully set up a standard curve dilution series that takes...increase the accuracy of your experiment.  Finally, carefully analyze the data and use the trendline that's ...

  4. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...translated protein. As depicted in the accompanying cartoon, they have a multitude of uses including (but not...handbooks for protein purification is provided by GE Healthcare Life Sciences. Addgene pages and blog posts ...

  5. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...chromosomal DNA. Chromosomal replication in bacteria is carefully regulated to ensure that it occurs at the appropriate...DNA replication in bacteria, so the process is carefully regulated by a mix of positive and negative regulators...

  6. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...This post was originally written by Maria Soriano-Carot in September 2016 and was updated by Rachel Leeson... career...

  9. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ... the cell type of interest (Atasoy et al. 2008; Cardin et al. 2009). Ligand regulated recombinase - keeping...biotechnology 16(7): 657–62. PubMed PMID: 9661200.  3. Cardin, Jessica A. et al. 2009. “Driving Fast-Spiking ...

  10. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...invaluable reagents with the scientific community. Note: Cary Valley and Mary Gearing contributed to the update...PMC4660269. 7. Joung J, Ladha A, et al., ‘Point-of-care testing for COVID-19 using SHERLOCK diagnostics....

  11. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...antibiotics is certainly a topic of great concern in healthcare today, but it doesn’t have to be some looming...resistance from the CDC. Antibiotic resistance can be scary to think about, but we can all do our part to learn...

  12. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...line is maintained for ~20 passages before being discarded and replaced with a new vial of early passage ...produced in the line will be taken offline and discarded. Confirmation of the transfer plasmid Addgene ...

  13. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...problem-solving process?  You may want to start by carving out time to tackle it. It’s easy to get distracted...been building your creativity all along. In my own career, my team has used these techniques to optimize ...

  14. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...Overview An immunoprecipitation reaction is typically carried out in one of two ways. Both methods utilize the...approach appealing, but the disadvantages must be carefully considered before using. For example, the tag ...

  15. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...promoter on the plasmid. Many commercial strains carry the DE3 lysogen, as indicated by the name of the...reasonable time-frame. For these cases, some strains carry an additional measure of control such as the pLys...

  16. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...Equipment needed: Mini PCR machines by DIY-Bio, P51 cardboard fluorescence visualizer with 9V battery Approach...fluorescently labelled RNA, the authors employ a P51 cardboard fluorescence visualizer that is powered by a 9V...

  19. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ... undergoing two rounds of gene-editing enables scarless introduction of an intended mutation while taking...4. Kwart, Dylan, et al. "Precise and efficient scarless genome editing in stem cells using CORRECT." Nature...

  20. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...issues Reproductive health Immigration Pollution Healthcare Science education To make scientists relatable...Scientists' Views PolitiFact: EPA head Scott Pruitt says carbon dioxide is not 'primary contributor' to global ...

  21. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...coupled with your technical skills, can open new career opportunities for you, if you can identify them...eye out for the phrase “I assumed they were taking care of that”, because that can indicate confusion around...

  22. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...available resources and give yourself time to carefully review your options. If possible, try to select...fluorescence-microscopy/   Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, ...

  23. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...First, CRISPR would need to be delivered to both cardiac and skeletal muscle cells, where precision editing... each observed the same positive phenotypes in cardiac and skeletal muscle. Even when the genome editing...

  25. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...intrinsic selectivity of the ligase active site and careful balance of reaction conditions to reduce the incidence...Probes), use of a high fidelity ligase, along with careful probe design and reaction condition optimization...

  26. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...cassette minimizes the sequence added, leaving a 34 bp “scar”, but currently requires additional experimental...efficiencies ranging from 11-100% of F1 animals carrying precise knock-ins, and homozygous knock-in animals...

  27. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...detergents such as triton-x, saponin, or tween. Take care when using strong detergents like triton-x and tween...while a droplet containing a negative cell is discarded. FACS can be performed aseptically allowing users...

  28. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...limitations or that other technologies need to be discarded but clearly we are in an era of neuroscience research... as the vector core at the University of North Carolina) that offer his constructs. Importantly, control...

  29. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...parts of the Cas9 enzyme.   Figure 1: A cartoon depiction of Cas9’s two major lobes, REC and NUC...process from start to finish:   Figure 9: Cartoon summary of Cas9 activity. The apo enzyme is shown...

  30. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ... PubMed Central PMCID: PMC5529245. Choi, V.W., McCarty, D.M., and Samulski, R.J. (2005). AAV Hybrid Serotypes... and Holzbaur, E.L.F. (2014). Axonal Transport: Cargo-Specific Mechanisms of Motility and Regulation. ...
Showing: 301 - 330 of 523 results