960 results

Electronic lab notebooks (ELNs) are useful tools that help researchers keep track of data. However, not all ELNs support the same functionality when it comes to reproducibility.

Jan. 17, 2019, 1:38 p.m.

The results from our #DeckTheLab social media contest are in! Read this blog post to see the Addgenie's favorite lab decorations.

Jan. 15, 2019, 2:19 p.m.

Addgene celebrates 15 years of scientific sharing. In this post, executive director Joanne Kamens reflects on the past and future of Addgene.

Jan. 14, 2019, 2:08 p.m.

Phage Directory is a directory of phage researchers and the phages they have in their labs with the goal of helping clinicians and scientists quickly get phages for phage therapy. Now, Phage Directory is also expanding to provide educational tools and resources to the phage community.

Jan. 10, 2019, 2:38 p.m.

Addgene reached its 15 year anniversary on January 8, 2019. In this blog post, find the top 15 requested plasmids since our founding. Many of these include lentiviral or CRISPR tools!

Jan. 8, 2019, 1:54 p.m.

Learn about CRISPR plant base editors for plants

Jan. 3, 2019, 1:35 p.m.

The CRISPR babies saga shows us that we need action to move forward in bringing CRISPR technologies from bench to bedside.

Dec. 20, 2018, 1:15 p.m.

This blog series showcases some of the new CRISPR tools we have at Addgene each quarter.

Dec. 18, 2018, 1:17 p.m.

The Addgene viral team has found a way to increase AAV yields through media supplementation of sucrose, an endocytosis inhibitor.

Dec. 13, 2018, 1:14 p.m.

If you're a scientist, you should use social media for networking and professional development! Read this blog post for some reasons why and five resources that can help you get started.

Dec. 11, 2018, 12:46 p.m.

Learn all about how to use a new plasmid for RNAi in C. elegans, tools for proximity labeling, RNA-based methods for detecting viral outbreaks, and tools for characterizing protein domain binding specificities.

Dec. 6, 2018, 1:34 p.m.

Cas14 was discovered by the Doudna lab. It cleaves ssDNA (but not dsDNA) in trans and can be adapted for a diagnostic toolkit such as DETECTR.

Nov. 29, 2018, 1:53 p.m.

Seeding Labs and Addgene collaborate to provide scientific resources, equipment, and plasmids to researchers in resource-limited settings. Read more to learn about the recipients of the Addgene-Seeding Labs plasmid grant this year.

Nov. 27, 2018, 2 p.m.

The purpose of this article is to explain what Unique Persistent Identifiers are, why they’re important, and to announce that Addgene now supports two different formats of plasmid identifiers and how they should be used.

Nov. 20, 2018, 8:17 p.m.

Learn how biotin is used in a variety of molecular biology experiments and makes it easier for researchers to label and purify macromolecules of interest.

Nov. 15, 2018, 1:50 p.m.

If you're a grad student looking to do a postdoc, you may be considering an academic or an industry postdoc. This article describes the pros and cons of both settings for your postdoc experience.

Nov. 13, 2018, 1:22 p.m.

Scientists are now using Addgene's plasmid repository as a source of sequence data sets. Researchers from MIT built a classifier that predicts whether a gene sequence is natural or synthetic and found that more and more genes are being sourced from farther away in the tree of life.

Nov. 8, 2018, 3:47 p.m.

Learn how stem cell researchers use reprogramming and transdifferentiation techniques to convert cells from one type into another.

Nov. 6, 2018, 1:12 p.m.

Plasmid addiction systems, used by bacteria to maintain plasmids in dividing cells, have been developed by scientists as molecular biology tools.

Nov. 1, 2018, 12:35 p.m.

Learn about the different types of stem cells in this guide. In particular, we discuss the pros and cons of using embryonic stem cells and adult stem cells and using induced pluripotent stem cells (iPSCs) as a model for regenerative medicine.

Oct. 30, 2018, 1:04 p.m.

Find tips for transforming arabidopsis with your plasmid construct of interest.

Oct. 25, 2018, 1:23 p.m.

Get tips for producing, purifying, and tittering AAV, a tick but useful viral vector of choice for many people working to express genes in mammalian cells.

Oct. 23, 2018, 11:49 a.m.

In Vivo Assembly (IVA) cloning uses the bacterial recombination pathway to allow any cloning procedure to be performed with a simple two-step, 2 hr protocol prior to transformation. This post covers the basics of the IVA cloning procedure, primer design, and tips & tricks for successful IVA cloning.

Oct. 18, 2018, 12:37 p.m.

Learn a variety of techniques you can use increase the fidelity of multi-part Golden Gate Assembly reactions.

Oct. 11, 2018, 12:30 p.m.

Erik Snapp, Director of Graduate and Postdoctoral Programs at Janelia Research Campus, gives advice on how to complete a successful postdoc and set yourself on a path to a successful academic career.

Oct. 9, 2018, 12:21 p.m.

The labs of John Doench and David Root have recently created CRISPR libraries to knock out, inactivate, or activate genes for genome-wide screens. These optimized libraries are effective for probing gene function while using fewer sgRNAs.

Oct. 4, 2018, 12:44 p.m.

Get tips on choosing the best lab for your graduate or postdoc work.

Oct. 2, 2018, 12:56 p.m.

Learn why codon usage bias exists and techniques you can use to avoid the detrimental affects of codon usage bias on heterologous protein expression - codon optimization and expression of alternative tRNAs.

Sept. 27, 2018, 1:09 p.m.

Addgene has recently reached the 1 million plasmids shared milestone. This post shares some of the social media around this feat and answers the questions: how much space does 1 million vials containing plasmids take?

Sept. 25, 2018, 2:39 p.m.

Learn how the Personal Genetics Education Project (pgEd) engages with various communities on biological science and technology. Get tips on how to work at a similar organization from pgEd's director of new initiatives, Johnny Kung.

Sept. 20, 2018, 12:17 p.m.

Learn all about how to regulate cell growth dynamics with optogenetics, the Riboglow platform for visualizing RNA production and localization, mCarmine, a new and improved red fluorescent protein, and the ACE reporter system for quantifying base-editing and Cas9 cleavage activity.

Sept. 18, 2018, 1:33 p.m.

Genome-wide CRISPR/Cas9 screens are a high-throughput systematic approach for identifying genes involved in a biological process. Recently the Bassik Lab at Stanford developed a new genome-wide CRISPR knockout screen to analyze unanswered questions about CRISPR screen design by including safe targeting guides and short gRNAs.

Sept. 13, 2018, 1:55 p.m.

Learn the basics of CRISPR ribonucleoprotein (RNP) preparation and delivery for your next gene editing experiment.

Sept. 6, 2018, 12:02 p.m.

Learn how the Journal of Emerging Investigators enables high school students to learn more about the scientific process and scientific publishing.

Sept. 5, 2018, 12:01 p.m.

Gene expression is often imprecise due to variations in gene copy number within the cell (either on plasmid or genome). New promoters from Chris Voigt’s lab enable stable gene expression regardless of copy number regardless of growth phase and whether they are expressed on the plasmid or chromosome.

Sept. 4, 2018, 12:53 p.m.

Though microbes are microscopic, there are many ways to visualize microbes. In this blog post, we summarize the latest tools in science communication to showcase microbes - foldscopes, agar art, and GIFs have all been recently use to bring microbiology to diverse audiences.

Aug. 28, 2018, 12:04 p.m.

GFP is the most widely used fluorescent protein, but there are several instances that limits its use. GFP requires oxygen, is sensitive to acid, and its large size can be problematic. When thinking about ways to label proteins, a different probe may be more appropriate.

Aug. 23, 2018, 12:05 p.m.

There are many plasmid tools available in molecular biology so how do you chose the right cloning method for you? Here, consider speed, cost, difficulty, efficiency, and the number of inserts when designing your cloning strategy.

Aug. 21, 2018, 12:31 p.m.

Learn how you can use RaPID to detect RNA-protein interactions and its applications in infectious and human genetic diseases.

Aug. 16, 2018, 12:40 p.m.

Learn how you can use the Spaghetti Monster fluorescent protein (smFP) tags for neuronal tracing and multicolor labeling of brain structures.

Aug. 14, 2018, 12:42 p.m.

Learn how AAVs can be used to trace neuronal connections.

Aug. 9, 2018, 1:04 p.m.

Find tips and best practices for producing lentivirus in your lab: from choosing a packaging system to optimizing transfection and improving titers.

Aug. 7, 2018, 12:31 p.m.

Learn how you can use the Biobits plasmids and freeze-dried cell extracts to teach students about molecular biology.

Aug. 2, 2018, 12:56 p.m.

Learn how and why Adeno-associated virus (AAV) has enabled breakthroughs in retinal gene therapy.

July 31, 2018, 12:55 p.m.

Learn how you can use kinase translocation reporters to monitor kinase activity at the single cell level.

July 26, 2018, 12:46 p.m.

Gamma-retroviral vectors and lentiviral vectors can be used in a wide variety of experiments including those involving genome engineering, RNAi, and inducible gene expression. In this post, learn about some of the most popular viral vectors available from Addgene and their uses.

July 24, 2018, 12:51 p.m.

Find great science communication tips from Nathan Sanders of ComSciCon, a communicating science conference series for graduate students.

July 19, 2018, 1:12 p.m.

Learn how you can use the Build Your Plasmid card game to teach undergraduate students about genetic engineering.

July 17, 2018, 12:23 p.m.

Learn some best practices to keep your mammalian cell lines free of contaminants and your experiments running smoothly.

July 12, 2018, 1:09 p.m.

Learn how electronic lab notebooks can make your lab life a little easier and find tips to choose the best the electronic lab notebook for your needs.

July 10, 2018, 1:08 p.m.