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We narrowed to 812 results for: APP

Showing: 501 - 550 of 812 results

  1. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ...reveal the winning entries on Twitter and Instagram! Happy decorating, all, and to all a good light!    🤩 ...set a challenge to my lab mates. They did not disappoint. #deckthelab pic.twitter.com/X4eKUGnQMe— Alex...

  2. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ... Molecular biologist with Biogen interested in applying CRISPR for mouse genome editing. Post: Generating...Generating Mouse Models Using CRISPR/Cas9   Erik L. Snapp, Associate Professor at Albert Einstein College of...

  3. Summer Fun at Addgene!

    Type
    Blog Post
    Published
    May 12, 2022, 1:15 p.m.
    ... Dives” series comes in! We’ll take a common application or concept and go beyond the basics - diving ...you’re ready for the next steps on some common applications, join us as we get into the weeds on some of...

  4. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...an organism’s genome usually for a research or applied goal. Components of CRISPR: gRNAs: Sequences ...produce either a gRNA, a nuclease, or both. Applications of CRISPR: Cut: CRISPR can be used to cut the...

  7. AAV Packaged on Request is Here!

    Type
    Blog Post
    Published
    Jan. 10, 2025, 1:01 p.m.
    ... in 100 µL aliquots, so the volume options will appear as 10, 25, or 50 units.  Once your request is received... you find this service useful in your research. Happy vectoring! ...

  8. Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana

    Type
    Blog Post
    Published
    Dec. 20, 2016, 3:30 p.m.
    ...awarded plasmids from Addgene as a successful applicant to the Addgene, Seeding Labs Plasmid Grant. For...scientist's molecular biology needs and why Kwabena applied for the Addgene, Seeding Labs plasmid grant. 7:...

  9. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    Published
    Sept. 1, 2016, 2:30 p.m.
    ... certain mystique that makes them difficult to approach for the youngster just starting in the lab, but... a graduate student? 4:50 - 8:00 : How did you approach doing rotations? 8:01 - 11:25 : How did you decide...

  11. Addgene Stands with the Scientific Community

    Type
    Blog Post
    Published
    April 23, 2025, 1:30 p.m.
    ...when budgets are constrained. This collaborative approach eliminates redundant work, saving time and allowing...be listed as direct costs from a CRO in grant applications, addressing the new funding structures and indirect...

  12. Bringing Sustainable Practices to the Lab: Easy Action Items

    Type
    Blog Post
    Published
    April 22, 2019, 12:26 p.m.
    ...night, so why leave your lab equipment on? This applies to water baths, computers, and more. Consolidate...to the Addgene blog Learn about other creative approaches for lab sustainability Resources on Addgene....

  13. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    Published
    March 26, 2015, 3:24 p.m.
    ... shipped! That's a 1/2 million plasmids sent to happy scientists around the world in ~10 years! We're ... as of March 26, 2015. How does Addgene make it happen? Our awesome team of Addgenies! Our team recently...

  14. Fueled by Coffee at #SfN14

    Type
    Blog Post
    Published
    Nov. 18, 2014, 7:15 p.m.
    ...talks, symposiums, poster sessions, exhibit booths, happy hour socials, and late, late night socials. As a...depositing scientists at #SfN14. And we've been so happy to hear that we've been able to help their labs ...

  15. Addgene's Website Update

    Type
    Blog Post
    Published
    April 8, 2024, 2:15 p.m.
    ...: The Education & Tools menu Further down What happens if you do scroll down? You’ll find Blugene, as ...through the yellow popup at the bottom of the page. Happy surfing!   This post was written by Addgenies Jo...

  18. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ... to curate the perfect library, obtained the appropriate cDNAs from other researchers, and cloned them... make the library usable for pooled screens. Applications of the cancer pathways ORFs kit Wood and his...

  21. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ...neurons expressing nLightG or nLightR before/after application of NE (10 μM) and corresponding pixel-wise ΔF...GFP), and nLightR uses a red fluorescent protein, mApple. Kagiampaki et al. found nLight is suitable for...

  23. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    Published
    April 1, 2014, 5:33 p.m.
    ...emphasizes that people should “follow their passions, be happy, and match their personal goals to their career ...analytical and critical thinking skills that are applicable to a variety of careers outside of academia. ...

  24. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    Published
    June 10, 2025, 1:15 p.m.
    ...individualized optimizations, safety testing, and approval. In contrast, a system that can deliver a healthy...could provide a streamlined, one-size-fits-most approach. evoCAST is an important step toward that kind...

  25. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    ...list of hundreds of different jobs for students to apply to, and after a grueling two months of job hunting...meetings, when Melina, our CSO and co-founder, approached me and asked about my current projects and personal...

  26. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ...willingness of the team to adopt various tools, this may happen over email or using rapid communication tools such...access data and drafts of manuscripts and grant applications in real-time so they can provide input at the...

  27. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    Published
    Feb. 14, 2019, 2:35 p.m.
    ... at least we can reduce the rate at which that happens and reduce the manufacturing of new containers,...bin or box in your tissue culture room for the wrappers and ship them back. Start a glove recycling ...

  28. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ...mutation on the receptor ACVR1. ACVR1 triggers appropriate bone regeneration in healthy patients. However...antibody that binds Activin A and interferes with inappropriate activin-ACVR1 interactions prevents FOP bone...

  29. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    Published
    March 31, 2015, 4:08 p.m.
    ...managers and some take on leadership roles. You may be happy with a primary role of doer for your entire career...they miss the hands-on bench work. To really be happy, you may need to find a way to do, manage and lead...

  30. Management for Scientists: Giving Feedback

    Type
    Blog Post
    Published
    Feb. 6, 2015, 2:46 p.m.
    ...respond to the employee’s level of discomfort appropriately to get the message across. Listen to our podcast...to you. What are We Learning: What would have happened if _________? What would have been a better outcome...

  32. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...and can be used in place of Ampicillin in most applications. Antibiotics vary in their sensitivitly to heat...hotter than about 55oC and store plates/stocks wrapped in foil if a light-sensitive antibiotic like Tetracycline...

  35. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...not silenced.    Find CellTag libraries here!  Applications of CellTag The Morris Lab developed CellTag ...References Biddy, Brent A., et al. "Single-cell mapping of lineage and identity in direct reprogramming...

  36. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...kinetics) that makes them suitable for different applications. We recently released a new variant of jGCaMP7... been developed and are suited for different applications. The iGABASnFR GABA sensor (pAAV.hSyn-FLEX.iGABASnFR...

  37. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ... work.  And while these pieces aren't always appropriate for your formal scientific communications, they...still get invested in the journey? The same thing applies for science storytelling. You can introduce your...

  38. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    ...categories: ones induced by the researcher and ones that happen naturally.  Sample Denaturing  First, let’s think...SDS-page gel. As the protein unfolds, new epitopes may appear, while others may get hidden. Monoclonal antibodies...

  40. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...into two cells. Viral vectors Viruses are quite appealing as molecular biology tools… if one isn’t too bothered...on the Addgene blog Viral Vectors 101: Viral Applications Viral Vectors 101: Viruses as Biological Tools...

  41. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...the gRNA design process and rules for various applications is necessary going forward. The standardization...Alt-NHEJ), has been harnessed for CRISPR knock-in applications. CRISPR 101: Targeting RNA with Cas13a (C2c2...

  42. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...response to customer feedback. An example of this approach is SnapGene’s algorithm for detecting common features.... We are working on a way for SnapGene users to apply features from a reference strain to newly sequenced...

  43. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...can do better, no grant writing Job changes can happen regularly (every 4-6 years is common), but this...issues can create interpersonal issues (one bad apple can change entire culture) Specific requirements...

  44. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...“A” overhang insert, the magic of TOPO cloning happens. Basic procedure Let’s break down the steps needed...1658796. PubMed Central PMCID: PMC52876. 2. Novel approach to molecular cloning and polynucleotide synthesis...

  47. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...protein-only transduction method and its potential applications to CRISPR/Cas9 genome editing.   Protein-only...Diego D'Astolfo. CRISPR/Cas9: a potential application of iTOP Having tested iTOP using Cre-lox recombination...

  48. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...multidisciplinary, collaborative, and translational approaches for scientific tool and technique development...leadership, management, and communication. A great approach to fully understand and acquire competitive skill...

  49. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...years! This has helped us develop a fast, agile approach, which we’ve updated and refined as new technology...and our MTA YouTube video for more information. Happy transferring!    Many thanks to our Tech Transfer...

  50. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...for additional best practices. Keep your cells happy - Make sure your cells are as healthy as can be ...AAV-mediated anterograde transsynaptic tagging: mapping corticocollicular input-defined neural pathways...
Showing: 501 - 550 of 812 results