We narrowed to 831 results for: APP

...to specific DNA sequences. Non-editing CRISPR applications direct catalytically dead dCas9 fused to a variety... publication at Addgene. Klann TS, Black JB, Chellappan M, Safi A, Song L, Hilton IB, Crawford GE, Reddy...Ruan K, Wang F, Xu G, Hu R (2016) A CRISPR-based approach for targeted DNA demethylation. Cell Discovery...

...for sphingomonads and other Alphaproteobacteria. Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi...cumate-inducible gene expression system for Bacillus. Appl Microbiol Biotechnol. 2019 Jan;103(1):303-313. doi...expression of genes for metabolic engineering applications in Saccharomyces cerevisiae.” FEMS Yeast Res...

...cross-team guy” at Addgene, an unofficial title that I happily embrace (and admittedly, I may have started that...identify as one.  But some time around 10th grade, I snapped out of the phase of my life that had me listening...you’re part of a team - it will just make you a happier person. What does a good team need? Besides that...

...have been increasingly popular in fundamental and applied research since their first use in the early 90’... at transferring the gene of interest to the appropriate cells, it was obvious that its safety was a disadvantage...either tTA or rtTA (FUW-M2rtTA). In this kind of approach, a population of cells might express only the ...

...et al (2). In this post, I’ll cover two great applications of the NLuc technology. The first couples NLuc...the coupled luciferase. This strategy was first applied using RLuc8, an engineered Renilla luciferase, ...fluorescence. The paper reports a wide variety of applications of the Enhanced Nano-Lanterns including monitoring...

...process is favored over the other. Cell conversion happens via: ReprogrammingA differentiated somatic cell...cells into another cell type. However, these two approaches differ in several ways. Below is a table listing...: 26042384. 27. Nejadnik, H., et al., Improved approach for chondrogenic differentiation of human induced...

...huge innovation, there was a limitation to this approach. CBEs and ABEs are base transition editors. They...GBEs), Xueli Zhang’s lab, returned with a unique approach to improving C → G editing. Instead of fusing ...abasic site. In this post, it refers to the same approach as a CGBE. BER Base Excision Repair A cellular...

...chance that all the edits you want to make will happen.  There are many reasons to multiplex gRNAs, some...adjacent to the sites. When digested with the appropriate Type IIS enzyme, the unique flanking overhang...subsequent “bricks” to be added one by one. This approach is especially useful for combinatorial analyses...

...Thinking about using antibody-based applications in your experiment...but not sure where to start or ...or quick refresher on — these common antibody applications.  Thanks for watching, and we hope you found...

...wintertime festivities! Help us spread some cheer by snapping a photo of you and your labmates celebrating the... the New Year. Will your lab be a lucky winner? Happy holidays from all of us at Addgene! We look forward...

... Why #DecktheLab? Help us spread some cheer by snapping a photo of you and your labmates celebrating the... the New Year. Will your lab be a lucky winner? Happy holidays from all of us at Addgene! We look forward...

... when Communicating with Non-Scientists How to Approach Different Audiences Listen to the SITN Podcast...Addgene Blog Check Out our Interview with Science Rapper Tom McFadden Learn about SITN's work at DayCon ...

...Performing In Vivo CRISPR Screens Using the FITS Approach Caitlin Faulds, who introduced the very first...Modular Cloning and Plasmids 101: Modular Cloning Applications and Kits We are very grateful for each and...

... Molecular biologist with Biogen interested in applying CRISPR for mouse genome editing. Post: Generating...Generating Mouse Models Using CRISPR/Cas9   Erik L. Snapp, Associate Professor at Albert Einstein College of...

... specificities, from target-specific ones to application-specific ones to anything goes. We've shared ...repositories and search engines Website Targets Application Purpose Notes Antibodypedia Any Any...

...reveal the winning entries on Twitter and Instagram! Happy decorating, all, and to all a good light!    🤩 ...set a challenge to my lab mates. They did not disappoint. #deckthelab pic.twitter.com/X4eKUGnQMe— Alex...

...with your manager early on. Chapter 4: Crystal's approach to management and the importance of realizing ... also adapting to needs as they arise. Tips on applying for industry jobs and deciphering job listings...

... find plasmids badge appear So how do you make this magical Addgene badge appear? First you’ll need to...

... Dives” series comes in! We’ll take a common application or concept and go beyond the basics - diving ...you’re ready for the next steps on some common applications, join us as we get into the weeds on some of...

...an organism’s genome usually for a research or applied goal. Components of CRISPR: gRNAs: Sequences ...produce either a gRNA, a nuclease, or both. Applications of CRISPR: Cut: CRISPR can be used to cut the...

... certain mystique that makes them difficult to approach for the youngster just starting in the lab, but... a graduate student? 4:50 - 8:00 : How did you approach doing rotations? 8:01 - 11:25 : How did you decide...

... detect a specific molecule. By combining this approach with receptors for problematic ligands, crops ...Gehan’s team also assessed hyperspectral imaging approaches for detecting plant pigmentation from an aerial...

... in 100 µL aliquots, so the volume options will appear as 10, 25, or 50 units.  Once your request is received... you find this service useful in your research. Happy vectoring! ...

...awarded plasmids from Addgene as a successful applicant to the Addgene, Seeding Labs Plasmid Grant. For...scientist's molecular biology needs and why Kwabena applied for the Addgene, Seeding Labs plasmid grant. 7:...

...that it is also possible that their system may be applied to efficient viral vector-mediated multiplex genome...genes at the same time.” For more details on how to apply the new system, see Yamamoto and Sakuma’s protocol...

...when budgets are constrained. This collaborative approach eliminates redundant work, saving time and allowing...be listed as direct costs from a CRO in grant applications, addressing the new funding structures and indirect...

...night, so why leave your lab equipment on? This applies to water baths, computers, and more. Consolidate...to the Addgene blog Learn about other creative approaches for lab sustainability Resources on Addgene....

... shipped! That's a 1/2 million plasmids sent to happy scientists around the world in ~10 years! We're ... as of March 26, 2015. How does Addgene make it happen? Our awesome team of Addgenies! Our team recently...

...talks, symposiums, poster sessions, exhibit booths, happy hour socials, and late, late night socials. As a...depositing scientists at #SfN14. And we've been so happy to hear that we've been able to help their labs ...

...: The Education & Tools menu Further down What happens if you do scroll down? You’ll find Blugene, as ...through the yellow popup at the bottom of the page. Happy surfing!   This post was written by Addgenies Jo...

...By Baiyang Liu and James Chappell, Rice University. For decades, we’ve been designing experiments around...This post was written by Baiyang Liu and James Chappell at Rice University, whose team developed SynORI...

...effective practices and disseminating career training approaches in a way that can be implemented widely in the...training programs. I believe in the academic apprenticeship system as a training ground for scientists,...

...where I foster dogs and help screen dog adoption applications. My most recent foster dog was with us for two...cancer through the sale of art. Past volunteer appointments included the Belmont Human Rights Commission...

... to curate the perfect library, obtained the appropriate cDNAs from other researchers, and cloned them... make the library usable for pooled screens. Applications of the cancer pathways ORFs kit Wood and his...

... swing dance event,” Zheng says (networking can happen anywhere!). The pair quickly teamed up to create...researcher may only know how to prepare phage for one application but not another or they may have only used phage...

...neurons expressing nLightG or nLightR before/after application of NE (10 μM) and corresponding pixel-wise ΔF...GFP), and nLightR uses a red fluorescent protein, mApple. Kagiampaki et al. found nLight is suitable for...

...adaptive bacterial immunity and its potential applications in rewriting the genome. Their award acceptance... area will accelerate the launch of clinical applications. During the panel discussion, Dr. Mello took...

...emphasizes that people should “follow their passions, be happy, and match their personal goals to their career ...analytical and critical thinking skills that are applicable to a variety of careers outside of academia. ...

...list of hundreds of different jobs for students to apply to, and after a grueling two months of job hunting...meetings, when Melina, our CSO and co-founder, approached me and asked about my current projects and personal...

...mutation on the receptor ACVR1. ACVR1 triggers appropriate bone regeneration in healthy patients. However...antibody that binds Activin A and interferes with inappropriate activin-ACVR1 interactions prevents FOP bone...

...individualized optimizations, safety testing, and approval. In contrast, a system that can deliver a healthy...could provide a streamlined, one-size-fits-most approach. evoCAST is an important step toward that kind...

...willingness of the team to adopt various tools, this may happen over email or using rapid communication tools such...access data and drafts of manuscripts and grant applications in real-time so they can provide input at the...

... at least we can reduce the rate at which that happens and reduce the manufacturing of new containers,...bin or box in your tissue culture room for the wrappers and ship them back. Start a glove recycling ...

...managers and some take on leadership roles. You may be happy with a primary role of doer for your entire career...they miss the hands-on bench work. To really be happy, you may need to find a way to do, manage and lead...

...respond to the employee’s level of discomfort appropriately to get the message across. Listen to our podcast...to you. What are We Learning: What would have happened if _________? What would have been a better outcome...

...guarantee that their experiments will work (yes, this happens): Science is hard. Chris Voigt, another Addgene...every conceivable background at the meeting. I appreciated all the feedback I received from scientists, ...

...and can be used in place of Ampicillin in most applications. Antibiotics vary in their sensitivitly to heat...hotter than about 55oC and store plates/stocks wrapped in foil if a light-sensitive antibiotic like Tetracycline...

...can do better, no grant writing Job changes can happen regularly (every 4-6 years is common), but this...issues can create interpersonal issues (one bad apple can change entire culture) Specific requirements...

...into two cells. Viral vectors Viruses are quite appealing as molecular biology tools… if one isn’t too bothered...on the Addgene blog Viral Vectors 101: Viral Applications Viral Vectors 101: Viruses as Biological Tools...

...“A” overhang insert, the magic of TOPO cloning happens. Basic procedure Let’s break down the steps needed...1658796. PubMed Central PMCID: PMC52876. 2. Novel approach to molecular cloning and polynucleotide synthesis...