We narrowed to 801 results for: DES

...is absolutely fascinating to see the living art designed by creative minds with a (I assume) very steady...Our Microbiology Resource Page CRISPR plasmids designed for use in bacteria Deposit Your Microbiology ...

...characteristic radiance for some time – there are records describing them dating back to the first century A.D. – ...mechanistic understanding of GFP’s function. His lab described the protein’s structure and also discovered many...

...knew very early on that I wanted to be an artist/designer. But there were a few teachers and 'guidance counselors...Don't worry about the rest.”- Jason Snair, Product Designer On the job search “Always go for the job you want...

... cas1, cas2, cas4, and a new gene cas14. cas14 encodes for a small Cas protein (40-70 kDa), which is half...Harrington, Lucas B., et al. "Programmed DNA destruction by miniature CRISPR-Cas14 enzymes." Science 362.6416...

...light-inducible transcriptional effectors (LITEs) designed to bind specific genes and turn them on or off...News & Views Andreas Möglich and Peter Hegemann described several advantages of the new system: "First,...

...collect in those red biohazard bags, typically is destined for a landfill. However, Triumvirate Environmental...Then, they use this material to produce various grades of plastic lumber and distribute it to industrial...

...on the Addgene blog and have created and updated guides on various topics in biology over the past year...containing these fluorescent proteins at Addgene. 7. Designing Your Chalk Talk for the Academic Job Interview...

...choice picks this month, I’m picking two posts that describe methods to make single base edits in DNA. The ...from Cornell University, is a bit old school and provides a thorough walkthrough of how you can use PCR ...

...wall of green algae (Soo et al., 2015). There, it divides into two cells. At the same time, it sucks out ...1983). Grasshopper parasite The parasitic worm Spinochordodes tellinii‘s larvae makes its home inside grasshoppers...

...infection, she shared that tweet with Jan Zheng, a UX designer that she knew. “We had actually met at a lindy...to possess the correct phages will test. These includes labs with a large number of different phages or...

...LaFleur, et al. 2024.   The X-CHIME deposit also includes pXPR_071, a plasmid that leads to higher titer...blog CRISPR101: Validating Your Genome Edit How to Design Your gRNA for CRISPR Genome Editing CRISPR Cheat...

...interactive game that Addgene employees created and designed for the Hall of Human Life at the MOS. (If you...all the fixings), as well as plenty of delicious desserts. We enjoyed a video highlighting the changes at...

...are the three main takeaways from the paper for designing experiments with AAV: 1. Getting your viral vector...AAV from our viral service can share experimental design and data. With these different approaches to open...

...new Help Center! Created using the same reliable Zendesk system that powers our email support, this portal...your name and email address, after which you can describe your issue and press send. Doing so will bring...

...Improving Agrobacterium binary vectors Vectors designed for use in Agrobacterium are termed binary vectors...ideal for large gene transfer. The M24 promoter provides constitutive, high-level gene expression and is...

...have hollow interiors and a protein shell that excludes water but is gas permeable. These vesicles are...sequentially exposed to acoustic pulses with high enough amplitudes to first collapse arg2 and then arg1 vesicles...

...when a high degree of signal to noise is desired. It also provides an excellent additional measure of DNA...

... Being an SME is often like being a jack-of-all-trades. “As a subject matter expert in the educational...the courses encompass what they want to teach.” Besides working with students and faculty, Caitlin also...

...repository and serving scientists better. That includes keeping fees as low as possible to ensure that...processes, distribution, and customer service) Addgene provides unmatched customer service that is consistently...

... favors the breakage of the hydrogen bonds, destabilizing the molecule and favoring the separation of ...nuclease free water is a great option because it provides a great versatility on how to use your plasmid...

...optimized for 2-photon imaging of voltages. This includes monitoring of fast bursts, as well as slow up/...KCRs), from a fungus-like protist Hyphochytrium catenoides (Govorunova et al. 2022). The authors show that...

...camelids too, though not just for the reasons the UN describes. In honor of the Camelid family, I wanted to take...of times on this blog over the years and have described how they compare to other antibodies and affinity...

...experience is in both vaccine subunit design and therapeutic biologic design (ADCs) along with pre-clinical ...

...come. Some Freshers Fairs can include fairground rides and other fun activities and even invite members...its members to become involved with. The society provides talks and career support for a range of different...

...transposon system is one such system which has been designed to perform gene transfer in vertebrates. The system...whose use is only likely to grow in the coming decades.  References 1. Kowarz E, et al. Optimized Sleeping...

...the measure of work is not just time spent at the desk, but it is worth it. This requires a focus on deliverables...characteristics of scientists in the work place as described by Sebastiano Massaro in his Nature Biotechnology...

...good and bad data, and knowing how to follow the design, execute, analyze, repeat pathway that is required...critically before diving in. Research projects are designed to train graduate students and postdocs to think...

...when the cells are grown on selective media, and provides those bacteria with a pressure to keep your plasmid...gene and acts as a marker for the mutation. When designing these types of experiments it is best practice...

...PDF at the end of this blog post. This document includes a list of questions to ask your potential advisors...some good pre-work you can find a lab that will deserve your hard work and an advisor that will be a lifelong...

...transport from the cell surface. Their toolkit includes a set of plasmids each containing an anti-GFP ...from A. victoria the functionalized nanobodies described here can be used to target any protein fused with...

...such as broccoli, cabbage and kale to name a few. Despite Arabidopsis’ utility to science, and its well-known...kicked off with the release of 20 education kits designed for use in K-12 and college-level instruction....

...one list can ever encompass them all. We can’t designate non-academic jobs as “alternative” anymore. ...Develop expertise areas quickly Jack/Jane of all trades More sources of training and resources within...

...of my review - "The manuscript by ZZZ et al., describes ... The authors share a wealth of data on ... ...Additionally, you can ask that data and resources described in the manuscript are accessible to the community...

...without end-point sequencing assays.  This tool was designed by putting together the coding sequence of a mCherry...MYC-CRISPR library targets E-boxes genome-wide. It was designed based on MYC-ChIP-seq data from several MYC-dependent...

...first NIH grant that Addgene has received. Addgene prides itself on being financially self-sustaining through..., Addgene’s interim Executive Director. “NABOR provides a new model for the creation and acquisition of...

...the Addgene website. blog.addgene.org The blog provides tips for working with viruses including advice...on each viral prep’s specific material page and includes western blots as well as other expression data...

...transduced using only protein. Read on for a description of the lab’s iTOP protein-only transduction method...transduction by osmocytosis and propanebetaine) to describe this method of protein transduction. Later studies...

...published in Nature Methods, Boyden and his team describe the discovery of a light-sensitive opsin from ...then, there has been a steady stream of papers describing new photosynthetic or photosensory molecules ...

...if you end up concentrating your AAV above your desired titer and want to dilute it, err on the side of...less buffer than you would to achieve the exact desired concentration to avoid over-diluting, and make ...

...form concatemers in later steps. This new NEB kit includes a step that both fragments the DNA and prevents...of the workshop, NEB gave a brief presentation describing the technology. Then we split into pairs to actually...

... determine if proteins are targeted to their destination co- or post-translationally. TRICK: Visualizing... usually translated in a specific compartment. Despite these limitations, these techniques represent powerful...

...references and web links from my first post and start designing your first fly experiment! Many thanks to our...PMID: 28704652. 6. V. Rodríguez, D. Didiano, C. Desplan, Power tools for gene expression and clonal analysis...

... Science. For each publication, Web of Science provides basic bibliographic information and uses its vast...citations due to the journal of publication. Table 1 provides an example calculation of the weights. Table 1...

...some point. Usually it comes up when a researcher decides on a new project and begins by trying to reproduce...they really want to do is get the paper off their desk and move on. Nevertheless, editors can and do play...

...best serotype for their particular study, which includes comparing the transduction patterns of different...fluorescence of the sensor. This “contrast” variant provides improved detection for tracking large populations...

... chromosome-specific loci in the genome and the guides are relatively unstable.   Figure 1: CRISPRainbow... the gRNA tetraloop and went through subsequent design iterations to increase stability and minimize misfolding...

...seeking “public views on emerging IVF techniques designed to prevent the transmission of mitochondrial disease..., and they are not remembered kindly. However, despite many headlines questioning if He’s experiments ...

...The natural CRISPR locus of a bacteria host encodes multiple guide RNAs (gRNAs) on a single array to ...applications, we provide detailed manual and online design examples of using Addgene plasmids hCtRNA_FT (#186715...

...visiting role is because the host lab or institute provides something that you can’t obtain at your current...skills and equipment! Preparing for your visit   Design and communicate a research plan Your time at the...

...biomass production techniques in the following decades, Phillips made the decision to release the K. phaffii...of K. phaffii lacks certain terminal mannose glycopeptides (which most other yeast possess) that are problematic...