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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 610 results for: STO;

Showing: 501 - 550 of 610 results

  3. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ... some exist as dsRNA. Still others use dsDNA to store their genetic information. All of these viruses ...

  4. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...sample(s) are resuspended in water or TE buffer, store them in a -80°C freezer to prevent RNA degradation...

  8. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ... within the FAH gene was precisely corrected, restoring expression in the liver.  Read the paper in Nature...

  9. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...Fishman JB, Berg EA (2019) Antibody Purification and Storage. Cold Spring Harb Protoc 2019:pdb.top099101. https...

  13. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...science instructor at The Innovation Institute in Boston. When Eugenia is not by the microscope she likes...

  14. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...the 4D nucleome. Visualizing specific loci has historically been difficult, as techniques like fluorescent...

  24. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...Engineering a CRISPR Variant with PAM Flexibility History of CRISPR Cas – A Tale of Survival and Evolution...

  25. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ... inspire further-improved versions. Thanks to Christophe Leterrier, whose FocalPlanes piece on mStayGold...

  28. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...biggest benefits of the Biomek FxP is it’s customizability. It has a large deck that can house many different...

  30. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...significantly higher than the same prep that has been stored in the freezer. If you plan to freeze a batch of...

  33. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...a genetic component. The VSD components have historically come from a variety of sources – voltage-gated...

  34. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...experimental therapies or pain relief, straight to the stomach. This technique involves administering medication...

  40. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ... BLAST options and for our suggested blastx customization. Similar to nucleotide sequences, proteins often...

  42. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...change your protein of interest.   Fig. 2: Stop re-cutting with PAM and guide RNA disrupting mutations...

  43. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...known pathogenic alleles, or introduce a premature stop codon to render an RNA nonfunctional. In their quest...

  45. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...Before you reach for that silica spin column, stop to consider some ways to purify DNA without a kit....

  47. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...containing buffer, polymerase and dNTPs, can be stored frozen: just defrost and add your primers and template...
Showing: 501 - 550 of 610 results