We narrowed to 746 results for: TRI

...than any other one. Luckily, you can do a lot to contribute to the relationship dynamic and set yourself ...notes on general protocol steps and other tips and tricks your mentor shares. Additionally, go ahead and ...

... libraries are typically DNA preps, but we do distribute several of our most popular CRISPR pooled libraries...cells at a concentrated seeding density while centrifuging. Spinfection transduction efficiency can vary...

...changes to a gene sequence can cause unexpected detrimental results to the protein. Codon optimization could...ZE, Kimchi-Sarfaty C (2011) Understanding the contribution of synonymous mutations to human disease. Nat...

... quick chat can be rather tricky (think people based in different countries). Here social media is a connector...

...kill your productivity. Harvard Business Review. Retrieved January 14, 2022, from https://hbr.org/2019/11...innovation on your team. Harvard Business Review. Retrieved January 14, 2022, from https://hbr.org/2019/07...

...This post was contributed by Samuel Mortensen, a PhD candidate at Northeastern University. Working with... 5.02 (2003): 103-115. Hu, Ching-yeh, et al. "Intrinsic GUS-like activities in seed plants." Plant cell...

...on to learn how the design of PHP.B may have contributed to this mouse-specific performance, why LY6A ...selection for PHP.B in C57BL/6J mouse strains likely contributed to the unintentionally selection of a virus with...

...This post was contributed by Erik Snapp, the Director of Student and Postdoctoral Programs at the Janelia...mechanistic, doable by 1-3 people in 1-5 years, and contribute to resolving the overarching knowledge gap. Aims...

...start answering questions. r/Science has pretty strict rules about not being too sales-y in posts which...term “implicit bias” which is apparently a troll trigger phrase these days). Redditors ask serious questions...

...vial of the desired cell line from a reputable distributor such as the American Type Culture Collection ...cloudy. Mycoplasma compete with host cells for nutrients and can alter expression of receptors, ion channels...

...Example steps include: permeabilization, antigen retrieval, and sectioning. Blocking Block is used to...validated antibodies? Many companies that produce and distribute antibodies validate them in a variety of applications...

...This post was contributed by guest blogger Daria Shcherbakova, a faculty member at Albert Einstein College...Shemetov et al., 2021). In addition to FRET, intensiometric calcium indicators NIR-GECOs were developed...

...doing so, they identified the nucleotides that contribute to the instability of Spinach, and were able ...superfolding Spinach2 reveals the dynamic nature of trinucleotide repeat–containing RNA." Nature methods 10.12...

...Molecular and Cellular Biology, harnessed these attributes of Cre-lox to create the Brainbow mouse neural...homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells. Snippert HJ, van der...

...sulfate proteoglycan (HSPG)3 AAV receptor (AAVR; not strictly required)2 Human fibroblast growth factor ...to Addgenies Brian O’Neill and Ina Ersing for contributing their expertise! This post was originally published...

... activated tools, can control everything from electrical activity to gene expression, and even CRISPR ...2019). Cortical layer–specific critical dynamics triggering perception. Science, 365(6453), eaaw5202. https...

....89.12.5547. PMID: 1319065; PMCID: PMC49329. Triezenberg SJ, Kingsbury RC, McKnight SL. Functional dissection...Park, S., Hill, C. et al. A LexAop > UAS > QUAS trimeric plasmid to generate inducible and interconvertible...

...all know that in the lab there are often little tricks that are essential for experiments but that nobody... not (Figure 4). 5. In theory you could use densitometric analysis to quantitate the intensity of the ...

...This post was contributed by Steph Guerra, a AAAS Science and Technology Policy Fellow at the Veterans... challenging if you are trying to apply for an outright job in a field where you have no specific scientific...

... that almost all off-target expression can be attributed to spontaneous recombination between homologous...the amount of leaky expression can largely be attributed to the level of spontaneous recombination between...

...This post was contributed by Erik Snapp, the Director of Student and Postdoctoral Programs at theJanelia... your time as they can result in spurious yet intriguing findings that fail to replicate. Plan experiments...

...your cells. While proteins themselves can emit intrinsic fluorescence when excited with ultraviolet (UV...use a dimmer fluorophore. However, these aren't strict rules and in practice the differences in brightness...

...(deprotonated) forms of the chromophore which contribute to the 2 peaks of wtGFP’s excitation spectrum...by engineering PA-GFP tagged to a mitochondrial matrix protein (mito-PAGFP) the Youle Lab was able to ...

...This post was contributed by guest blogger Pooran Dewari, a postdoc in Steve Pollard’s lab at the MRC...follow when planning epitope tagging experiments: Retrieve Ensembl Transcript ID(s) of your favourite gene...

... nuclease function. Stem loops 2 and 3 are not strictly necessary for in vitro cleavage, but they improve...Figure 3 crystal structure, reflecting the less stringent recognition mechanism that can tolerate mismatches...

...different fix/perms (methanol, PFA-saponin, PFA-triton). The fix/perm solutions can make a significant...compared, 4% PFA and 0.1% saponin; 4% PFA and 0.1% Triton X-100; and methanol, we found that the optimal ...

...chimeric offspring shows the ability of the iPSC to contribute to all three germ layers, and the ability of ...iPSCs to form functional germ cells. The highest stringency test for mouse iPSCs, tetraploid complementation...

... Abi run a Western blot and share tips for this tricky application.    You can check out all of our ...

...excited to share some good news with you: with contributions from depositors around the globe, we’re now ...

...bar is set very high from last year’s impressive entries, but we have faith our community of creative scientists...

...main purpose of the party was to celebrate the contributions of each and every Addgenie, from our co-ops ...

...have been shared over 140,000 times to over 75 countries. That’s a lot of CRISPR! As we’ve shared these...

...platform for scientists using AAV to find and contribute experimental information, such as titer, serotype...

...   “Unearthed Comics” by Sara Zimmerman   5)   “Beatrice the Biologist” by Katie McKissick       Sara Zimmerman...

...Instagram and we’re excited to share these creative entries as the winners of the 2019 contest. Agar art ...

...third day of CRISPR, Addgene gave to me: tips and tricks for designing gRNAs for CRISPR editing. On the ...

...awesome new technology they’re utilizing, and the tricks they’ve used at their lab benches. It’s these exchanges...

...! AAV purification by iodixanol gradient ultracentrifugation This video goes over AAV vector purification...

...Additional Addgene Podcasts Learn How You Can Contribute to the Addgene Blog Learn about Addgene Plasmid...

...your favorite topic. If you have any other cool tricks to make PubMed browsing even easier and more useful...

... post! In January, Addgenies will vote on the entries in both the classic lab and home editions. We will...

...learned to prioritize projects, and how she sets metrics for success. The importance of thinking about the...

... it. Can you guess each plasmid? Our lab paid tribute to the changing seasons – and they also invited...

...working with fellow nonprofit, Seeding Labs, to distribute plasmids to researchers in developing regions...

...now with more than 50% international plasmid distribution!) Chapter 8 - Opportunities to create repositories...

...discoveries and developments, and provide tips and tricks for new and seasoned molecular biologists alike...

...edition, the MicrobiGals created glow in the dark petri dish ornaments and microbe cookies! 🤗 🤗 🤗 We...

... To better highlight the great content contributed by our bloggers each and every month, we've decided...

...provide plasmid grants to researchers in developing countries. Today we’re proud to announce that we’ve selected...

...ineffective or unimportant. With so many big contributors to the sustainability problem, how can one little...