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Showing: 501 - 550 of 728 results

  1. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ... activated tools, can control everything from electrical activity to gene expression, and even CRISPR ...2019). Cortical layer–specific critical dynamics triggering perception. Science, 365(6453), eaaw5202. https...

  2. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 3:42 p.m.
    ...sulfate proteoglycan (HSPG)3 AAV receptor (not strictly required)2 Human fibroblast growth factor ...to Addgenies Brian O’Neill and Ina Ersing for contributing their expertise! References and resources References...

  3. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ....89.12.5547. PMID: 1319065; PMCID: PMC49329. Triezenberg SJ, Kingsbury RC, McKnight SL. Functional dissection...Park, S., Hill, C. et al. A LexAop > UAS > QUAS trimeric plasmid to generate inducible and interconvertible...

  4. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...This post was contributed by Erik Snapp, the Director of Student and Postdoctoral Programs at theJanelia... your time as they can result in spurious yet intriguing findings that fail to replicate. Plan experiments...

  5. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ...This post was contributed by Steph Guerra, a AAAS Science and Technology Policy Fellow at the Veterans... challenging if you are trying to apply for an outright job in a field where you have no specific scientific...

  6. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ... that almost all off-target expression can be attributed to spontaneous recombination between homologous...the amount of leaky expression can largely be attributed to the level of spontaneous recombination between...

  7. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...all know that in the lab there are often little tricks that are essential for experiments but that nobody... not (Figure 4). 5. In theory you could use densitometric analysis to quantitate the intensity of the ...

  9. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...(deprotonated) forms of the chromophore which contribute to the 2 peaks of wtGFP’s excitation spectrum...by engineering PA-GFP tagged to a mitochondrial matrix protein (mito-PAGFP) the Youle Lab was able to ...

  10. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...your cells. While proteins themselves can emit intrinsic fluorescence when excited with ultraviolet (UV...use a dimmer fluorophore. However, these aren't strict rules and in practice the differences in brightness...

  11. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ... nuclease function. Stem loops 2 and 3 are not strictly necessary for in vitro cleavage, but they improve...Figure 3 crystal structure, reflecting the less stringent recognition mechanism that can tolerate mismatches...

  12. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...different fix/perms (methanol, PFA-saponin, PFA-triton). The fix/perm solutions can make a significant...compared, 4% PFA and 0.1% saponin; 4% PFA and 0.1% Triton X-100; and methanol, we found that the optimal ...

  13. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...chimeric offspring shows the ability of the iPSC to contribute to all three germ layers, and the ability of ...iPSCs to form functional germ cells. The highest stringency test for mouse iPSCs, tetraploid complementation...

  14. New Western Blot Protocol Video!

    Type
    Blog Post
    Published
    March 28, 2023, 1:15 p.m.
    ... Abi run a Western blot and share tips for this tricky application.    You can check out all of our ...

  16. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    Published
    Dec. 2, 2016, 3:30 p.m.
    ...bar is set very high from last year’s impressive entries, but we have faith our community of creative scientists...

  17. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ...have been shared over 140,000 times to over 75 countries. That’s a lot of CRISPR! As we’ve shared these...

  18. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ...main purpose of the party was to celebrate the contributions of each and every Addgenie, from our co-ops ...

  19. We’re Going Viral This Week!

    Type
    Blog Post
    Published
    Oct. 14, 2019, 1 p.m.
    ...platform for scientists using AAV to find and contribute experimental information, such as titer, serotype...

  22. The Twelve Days of CRISPR

    Type
    Blog Post
    Published
    Dec. 22, 2020, 2:15 p.m.
    ...third day of CRISPR, Addgene gave to me: tips and tricks for designing gRNAs for CRISPR editing. On the ...

  29. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ... it. Can you guess each plasmid? Our lab paid tribute to the changing seasons – and they also invited...

  30. Addgene’s Blog Gets a New Look!

    Type
    Blog Post
    Published
    Jan. 25, 2021, 5:54 p.m.
    ...discoveries and developments, and provide tips and tricks for new and seasoned molecular biologists alike...

  31. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    Published
    Jan. 14, 2021, 1:30 p.m.
    ...edition, the MicrobiGals created glow in the dark petri dish ornaments and microbe cookies! 🤗 🤗 🤗 We...

  32. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    Published
    April 13, 2018, 12:51 p.m.
    ...now with more than 50% international plasmid distribution!) Chapter 8 - Opportunities to create repositories...

  33. Editor's Choice, July 2016

    Type
    Blog Post
    Published
    Aug. 5, 2016, 3 p.m.
    ... To better highlight the great content contributed by our bloggers each and every month, we've decided...

  37. Fueled by Coffee at #SfN14

    Type
    Blog Post
    Published
    Nov. 18, 2014, 7:15 p.m.
    ... labs save time and resources by storing and distributing their plasmids with the scientific community...

  38. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ... most recent cases, primarily in West African countries. Even that grim estimate might prove to be an ...

  40. Addgene's Website Update

    Type
    Blog Post
    Published
    April 8, 2024, 2:15 p.m.
    ...depositing data, instead of materials,  there’s a “Contribute to the Data Hub” link in the Deposit bar. Note...

  46. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ...this plasmid has a U6 promoter followed by BbsI restriction sites into which you can clone your 20 nucleotide...

  47. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ...disease processes. Get the PUF Assembly Kit! Tips and tricks For those interested to try the PUF Assembly Kit...

  49. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...can be applied non-invasively, its use is not restricted to cultured cells but extends to freely moving...
Showing: 501 - 550 of 728 results