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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 610 results for: ACE;

Showing: 201 - 220 of 610 results

  1. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    Published
    March 15, 2018, 12:59 p.m.
    ... a double-strand break, and when used with two adjacent gRNAs, can lower the probability of off-target... of the targeted region, whereas PAM-in designs place the PAMs closer together in the middle of the targeted...sites separated by 51-68 bp Use D10A for HDR Place intended insert in between nick sites Use 40 nt...

  2. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    Published
    Feb. 14, 2019, 2:35 p.m.
    ...your workplace and you could end up making a huge impact on how waste is handled. A lot of places have...on waste, but also on culture. Encourage your workplace to become a leader in sustainability. Sign up...

  3. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ... Many thanks to our guest blogger Sean Stacey! Sean D. Stacey is a Ph.D. student at East Tennessee State...This post was contributed by guest blogger Sean Stacey who recently attended both online and on site courses...

  4. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ... amazing job shuttling unwanted lab supplies to places that really need them.  When I was in graduate ...indefinitely at room temperature (thus also saving freezer space).  The procedure becomes less attractive when noting...very thin gel on a large glass slide using only surface tension.  It's a bit tricky to pour the right amount...

  5. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ... and other related resources. Check Out Angela Depace's Talk on "Precision and Plasticity in Mentoring...by @drmellivora, an excellent blog post about workplace bullying featured on the always interesting Tenure...thing to do is not go into their labs in the first place. The good news There are many excellent mentors ...

  7. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...tech companies using only laptops and some server space. But what if you want to be an entrepreneur in ... graduate students with innovative ideas in all facets of the entrepreneurial process in order to develop...you start at a disadvantage in the health science space if you don’t have an advanced degree behind your...

  8. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...therapeutic proteins. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft...complexity of antigen processing events that take place within an antigen-presenting cell like a T cell....

  9. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...The most commonly used yeast by biotech and pharmaceutical companies is K. phaffii (formerly known as ...the system. Since then, multiple biotech and pharmaceutical companies (along with some research laboratories...the expression of recombinant proteins by the pharmaceutical and biotech industries as a protein expression...

  10. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ...about how to solve this problem. I didn’t want to face another group of sleepy students at 8 a.m. many ...format I decided on was to have two teams of 4 people face each other on a single chosen clinical disease case...they agreed on each element to use, they had to place the selected card on the board game. They had 35...

  11. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ... that cell. Since there is a limited amount of surface area on each cell, the way to get more AAV in contact...expression. How do you achieve high titers in the first place? Some AAVs are known to be difficult to produce ...concentration filter column, make sure to rinse off the surface of the filter membrane with the surrounding solution...

  12. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...where the mutation can be placed. With other techniques, when mutations are spaced farther apart, it becomes... which is freely available online. Its simple interface can guide you through both experimental and primer...

  13. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...solution thawed, as the CO2 was confined to the tube headspace when the samples were frozen. This was clear by...the gradual change in pH visible at the sample interface upon thaw (Fig. 2). Before thawing, all the samples...tubes prior to thaw released the CO2 from the headspace, thus mitigating the pH effects of dry ice storage...

  15. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...targeted known residues on the outer surface to break dimerization interfaces (affecting many of the RFPs developed...discovered reprogramming discrepancies, which they traced to a 9 amino acid N-terminal variation in the Klf4...genes identified as non-essential. The knockouts replace entire protein-coding regions (excluding start ...

  16. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    Published
    Jan. 21, 2025, 2:15 p.m.
    ...One of the most pivotal decisions many PhDs face when transitioning from academia to industry is whether...requires deliberate preparation and a willingness to embrace new responsibilities. After my second postdoc,...permission to explore, reflect on your priorities, and embrace these transitions as an integral part of your broader...

  17. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...guided by a gRNA is useless without an adjacent PAM or protospacer-flanking sequence (PFS) in some cases...now, it’s time to cast aside those concerns and embrace a new resource to solve the problem: CasPEDIA, ...

  18. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...cell!)—ATP. There are biosensors for intracellular, extracellular, and even the ADP:ATP ratio. Most of... from dopamine to glutamate, all the way to acetylcholine. These sensors have been tested in vivo, in ...

  19. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    Published
    May 3, 2018, 1:48 p.m.
    ...HEPN-independent. These enzymes also do not require a protospacer flanking sequence, so you can target virtually...either an unprocessed or processed gRNA array (22 nt spacer with 30 nt direct repeat) mediating potent knockdown...dementia-associated tau, delivering dCasRx and a 3-spacer array targeting the MAPT exon 10 splice acceptor...

  20. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...substrate, they synthesized diacetyl coelenterazine-h. Unlike coelenterazine-h, diacetyl coelenterazine-h does... to the media. Cellular esterase then converts diacetyl coelenterazine-h to coelenterazine-h, producing...
Showing: 201 - 220 of 610 results