We narrowed to 295 results for: Ada

1. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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   Blog Post

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   June 6, 2019, 12:41 p.m.

   ...group inserted sgRNAs targeting various innate and adaptive immune genes into pXPR_053. They expressed this...

2. Targeted Mutagenesis with EvolvR

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   Blog Post

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   Feb. 21, 2019, 1:15 p.m.

   ...Nature 560.7717 (2018): 248. PubMed PMID: 30069054. Sadanand, Saheli. "EvolvR-ing to targeted mutagenesis."...

3. Viral Vectors 101: Biosensors

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   Blog Post

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   July 6, 2023, 1 p.m.

   ...measured GFP levels and converted that signal into a readable value. Types of biosensors So now that we know...

4. Viral Vectors 101: Viral Applications

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   Blog Post

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   April 13, 2023, 1:15 p.m.

   ...which target complementary mRNA sequence for degradation. Viral introduction of shRNA plasmids can induce...

5. Plasmids 101: Screens vs. Selections

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   Blog Post

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   Nov. 10, 2022, 2:15 p.m.

   ...and consider these questions to save yourself a headache later on. Enhancer and suppressor screens These...

6. Hot Plasmids - February 2022

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   Blog Post

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   Feb. 22, 2022, 2:15 p.m.

   ...mitochondrial (mito-mCherry-1xFLAG) localization. Adapted from Liu et al. 2021. Anti-FLAG and anti-HA...

7. Antibodies 101: Isotypes

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   Blog Post

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   Oct. 19, 2021, 1:15 p.m.

   ... constant domains. This switching helps B cells adapt during the course of an immune response since it...

8. Antibodies 101: Monoclonal Antibodies

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   Blog Post

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   June 29, 2021, 1:15 p.m.

   ...10.1093/ilar.46.3.258  Mould AP, Askari JA, Byron A, Takada Y, Jowitt TA, Humphries MJ (2016) Ligand-induced...

9. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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   Blog Post

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   May 19, 2020, 1:15 p.m.

   ... in much of the world, we have found ourselves adapting to a new way of doing things. These new processes...

10. CasPEDIA: A Functional Classification of Cas Enzymes

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    Blog Post

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    Jan. 25, 2024, 2:15 p.m.

    ...Comparison of different Cas enzymes using CasPEDIA. Adapted from Adler et al.   You can also search by ...

11. Viral Vectors 101: Inverted Terminal Repeats

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    Blog Post

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    Oct. 19, 2023, 1:15 p.m.

    ...that can make vector propagation and sequencing a headache. How do I maintain ITR integrity and prevent unwanted...

12. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

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    Blog Post

    Published

    Dec. 6, 2018, 1:34 p.m.

    ... dsRNAs target specific mRNA transcripts for degradation. Despite how easy these experiments are to physically...

13. Top Requested Lentivirus and AAV of 2016

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    Blog Post

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    Jan. 6, 2017, 3:56 p.m.

    ...PMC3785459. 2. Krashes MJ, Koda S, Ye C, Rogan SC, Adams AC, Cusher DS, Maratos-Flier E, Roth BL, Lowell ...

14. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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    Blog Post

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    June 2, 2015, 6:06 p.m.

    ...designed for plant expression, PTGs will likely be adaptable to many different biological systems. Although...

15. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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    Blog Post

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    Feb. 24, 2015, 7:50 p.m.

    ...Repression and Activation. Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes...

16. Will You Be My Mentor? Finding and Asking for Mentoring Support

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    Blog Post

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    Feb. 4, 2014, 3:50 p.m.

    ...ardor and attended to with diligence.” - Abigail Adams, 1780 There are potential mentors all around you...

17. Troubleshooting Your Plasmid Cloning Experiment

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    Blog Post

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    Sept. 24, 2019, 12:55 p.m.

    ...provided by the major companies selling PCR reagents. Nowadays commercial polymerases are very efficient, but...

18. All in a Twist: dsRNA

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    Blog Post

    Published

    Feb. 2, 2023, 2:15 p.m.

    ...Transcription, translation, and prone to degradation – those are the words that describe RNA! Double ...

19. Summer SciComm: Preprints

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    Blog Post

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    Aug. 16, 2022, 1:15 p.m.

    ...asapbio.org/preprints-and-covid-19 Ettinger CL, Sadanandappa MK, Görgülü K, Coghlan KL, Hallenbeck KK, Puebla...

20. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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    Blog Post

    Published

    Jan. 31, 2019, 1:32 p.m.

    ...cells promote oxidative stress leading to the degradation of one key enzyme (among others): caveolin 1,...