We narrowed to 256 results for: Alk

1. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

   Type

   Blog Post

   Published

   June 5, 2014, 3:32 p.m.

   ...few jobs available. So, we spent a lot of time talking about what we could do to make ourselves marketable...

2. Degrading DNA with Cascade-Cas3

   Type

   Blog Post

   Published

   Feb. 11, 2025, 2:15 p.m.

   ...s41587-019-0310-0 Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa, F., Shah, S. A., Saunders, S. ...

3. Production of Virus in Insect Versus Mammalian Cells

   Type

   Blog Post

   Published

   Nov. 5, 2024, 8:32 p.m.

   ... from typical insect cell preparations, which we talk about below. Processing The viral supernatant is...

4. Transferable Skills: Negotiation

   Type

   Blog Post

   Published

   March 3, 2022, 2 p.m.

   ...new grad student keeps using the machine? Let’s walk through a more specific example. Perhaps you and...

5. Transferable Skills Guide: Managing a Team

   Type

   Blog Post

   Published

   Jan. 30, 2018, 2:42 p.m.

   ...well done. Your report will (needs to) feel valued. Talk to your manager and find a mentor Your manager should...

6. Academic vs. Industry Postdocs

   Type

   Blog Post

   Published

   Nov. 13, 2018, 1:22 p.m.

   ...honest, I’ve never enjoyed grant-writing. The more I talked to people with industry experience, the more I ...

7. Plasmids 101: Knockout/Knock-In Plasmids

   Type

   Blog Post

   Published

   Dec. 1, 2016, 3:30 p.m.

   ...interest. To give you an idea of the process, we’ll walk through an experiment designed to knock out exon...

8. CRISPR 101: RNA Editing with Cas13

   Type

   Blog Post

   Published

   July 31, 2020, 12:30 p.m.

   ... editor that allows C to U edits (RESCUE). We’ll walk through how these tools were developed and potential...

9. Capturing a Moment with Marc Zimmer’s “The State of Science”

   Type

   Blog Post

   Published

   July 30, 2020, 1:15 p.m.

   ..., and Ed Boyden crop up in this book was quite a walk down memory lane for me. A brief window in time...

10. DNA Purification Without a Kit

    Type

    Blog Post

    Published

    Feb. 11, 2020, 2:15 p.m.

    ...silica columns from DNA purification kits. Kit-free alkaline lysis plasmid miniprep Starting material: 2 mL...

11. A Guide to Starting Your Own Journal Club

    Type

    Blog Post

    Published

    Feb. 6, 2020, 3 p.m.

    ...journal club. Some are led by a single individual who walks through the paper figure by figure. Some follow ...

12. Transferable Skills Guide: Identifying Your Transferable Skills

    Type

    Blog Post

    Published

    Jan. 11, 2018, 3:29 p.m.

    ...LinkedIn pages, given yourself the bathroom mirror pep talk, forgotten and then retrieved your lucky socks, ...

13. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type

    Blog Post

    Published

    Sept. 8, 2016, 2:30 p.m.

    ...age of GFP and its ilk, why should cell biologists talk to chemists? One reason is something called “the...

14. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type

    Blog Post

    Published

    July 8, 2025, 1:15 p.m.

    ... you make your plasmid construction with MoClo a walk in the park.  Before you begin, be sure to check...

15. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type

    Blog Post

    Published

    July 30, 2024, 1:15 p.m.

    ...after all, a delivery method. I would be remiss to talk about them without looking at what they’re delivering...

16. Antibodies 101: The Four ELISAs and When to Use Them

    Type

    Blog Post

    Published

    July 25, 2024, 1:15 p.m.

    ...enzyme such as horseradish peroxidase (HRP) or alkaline phosphatase (AP). Fluorescent tags may also be...

17. Viral Production at Addgene

    Type

    Blog Post

    Published

    Oct. 24, 2023, 1:15 p.m.

    ... preps available to request. In this post, we’ll walk you through how we produce both our AAV preps and...

18. Viral Vectors 101: Preparing Pooled Libraries

    Type

    Blog Post

    Published

    July 13, 2023, 1:15 p.m.

    ... be quite challenging to use! In this post, I’ll walk you through how to prep and transduce your pooled...

19. New CRISPR Tools: Cas7-11 and PASTE

    Type

    Blog Post

    Published

    Feb. 17, 2022, 2 p.m.

    ...Liyang Zhang, Christopher A. Vakulskas, John A. Walker II, Anastasia P. Kadina, Adrianna E. Zepeda, Kevin...

20. Transferable Skills Guide: Creativity

    Type

    Blog Post

    Published

    Jan. 18, 2022, 2:15 p.m.

    ...be as simple as a few minutes of meditation or a walk to the break room for a sip of water.  Fig...