...templates for the cloning process. Time spent actually doing the experiments are also generally comparable so...inexpensive DpnI enzyme. Otherwise you let your E. coli do the work for you. Traditional restriction enzyme ...are never any guarantees, some cloning techniques do tip the scales in your favor. For starters, Golden...

...insertions that could compromise function. When we do find mutations, we check to see if they affect the... confirms that these differences are expected and do not affect plasmid function, we will update information... They also differ from the CPS process in that we do not fully sequence all plasmids in the pool but instead...

...you, you may wonder which antibody should I pick? Do I go for the rabbit or the mouse? Is mouse IgG1 better...signal strength (Fig. 1). Monoclonal primaries If you do decide on a monoclonal antibody you may wonder, “...immunoglobulins and reduce the background staining but they do not always eliminate the issue (Lu 1998). Consequently...

...Get your voice out there Here’s one thing you can do today: Sign up for a Twitter account. Check out the...happening. 3) Volunteer – Step into science education Do you aim to reach young people? Try networking with...TED-Ed lesson. 6) Network – Mingle with pros Where do you meet like-minded science communicators? Attend...

... at this still relatively small nonprofit. If you do go longer than a few days without getting a response...particularly when it comes to technical questions. BUT, I do believe that scientists who contact customer service...Our Frequently Asked Questions Pages Learn What to Do with Your Plasmids Once You Recieve Them Get Help...

...tell, we don’t really know what circRNAs do yet or how they do it. Of course, No conference would be complete...work done by the ENCODE project consortium did not do a good job of seeing tRNAs because they contain “...

... SITN, we discuss some of the many things you can do start your own science communication student group...get more involved with your local community. What do Vini and Amy say is the quickest way to get things...

...environment without sacrificing performance or quality. To do this, we can use the ACT label, which is an eco-nutrition...approach to lab waste is something we all need to do. Remember, it was only 50 years ago that we routinely...

...views for the month of September! Historically we do better in September than in the summer months, but...phages are viruses that infect bacteria and that can do so with exquisite specificity (check out our interview...

...influenced by temporally ordered biochemical events. How do RSMs work? The state of an RSM is defined by the ...live, intact cells; the sequencing and qPCR methods do not. How might one implement RSMs? In our study (...that correspond to the desired inputs. Such sensors do not necessarily have to be based on transcriptional...that best suits your application. How efficiently do RSMs work? We tested a two-input (ATc and Ara) RSM...

...prepared me that those challenges would be from doing a remote internship during a pandemic. Before I ...role in the company. One of the things I wanted to do was create content that was unique but also brand...

... job descriptions clearly describe that the roles do not involve bench research. I am always surprised... 5) Apply for the job as requested in the posting Do this online and/or via email exactly as the job posting...

...received over 2,000 entries. Do you mix your passion for art and science? Do you look for the beauty in ...

...and tell your audience just that. Explain what you do know about a related topic, and let them know while...overwhelming than stepping into a sea of posters – where do you begin? Design a game plan Prior to attending ...poster you want to know more about? Interested in doing a postdoc in a presenter’s lab? Want to collaborate...

...it. Biotech companies are known to have tools that do this internally, but they are not available publicly...advised, there is little detail published on how to do it best. Scientists are typically advised to mutate...boosted our productivity in the Biohub. We hope it will do the same for you. Send us your questions or comments...

...Accuracy needed Do you need to call exact copy numbers or just general trends? How confident do you need to...researchers can use to quantify said copies. They do so using copy number variation (CNV) assays. There...

...blue to ultraviolet light, whereas chromoproteins do not fluoresce at all or fluoresce at very low levels...identified in sea anemone (Chan et al., 2006). Why do these organisms have chromoproteins? One reason may... mutation and excision of the vector backbone. To do this, rhamnose is added which induces expression ...

... on the inside of the cell. However, assuming you do need to permeabilize, you have a few options for ...organellar membranes. Created with BioRender.   How do I choose?! Ok, so now you know some of the possible...possible types of fixatives or permeabilizers, but how do you decide which ones to use? Here are some tips ...

...which are doing important work in our microbiomes. Given that viruses are everywhere and doing everything...we try to use them in an experiment and they don't do anything. Viruses are complicated and they perform...

...before using them to screen colonies. The best way to do this is by using your vector with and without an ...clones for later use. There are a few ways you can do this. If you are going to complete your colony PCR...Additional Resources on the Addgene Blog Learn to do Restriction Cloning Try SLIC cloning Learn about ...