We narrowed to 267 results for: INO

1. AAV Vector Quality Control: Going the Extra Mile with NGS

   Type

   Blog Post

   Published

   Sept. 12, 2017, 1:44 p.m.

   ...viral production. This recombination produces a minor pre-recombined population that does not require ...

2. Truncated gRNAs for Regulating Gene Expression

   Type

   Blog Post

   Published

   Jan. 10, 2017, 3:37 p.m.

   ...additional effector domains (4). Taken together, these minor gRNA alterations can be exploited to quickly and...

3. Tips for Using BLAST to Verify Plasmids

   Type

   Blog Post

   Published

   May 29, 2014, 1:29 p.m.

   ...blastx results start the alignment at a more distal amino acid. We consistently run our blastx searches with...

4. Antibodies 101: Producing Recombinant Antibodies

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   Blog Post

   Published

   Aug. 13, 2024, 1:15 p.m.

   ... though this can vary depending on the specific amino acid sequence (Maity et al., 2015). If your antibody...

5. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

   Type

   Blog Post

   Published

   Aug. 30, 2022, 1:15 p.m.

   ...https://doi.org/10.1177/33.8.3894499    Im K, Mareninov S, Diaz MFP, Yong WH (2018) An Introduction to...

6. Capturing a Moment with Marc Zimmer’s “The State of Science”

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   Blog Post

   Published

   July 30, 2020, 1:15 p.m.

   ... Aliyah Weinstein, Fabienne Furt, and Quintin Marcelino for their helpful discussion of this book....

7. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

   Type

   Blog Post

   Published

   July 1, 2025, 1:15 p.m.

   ...secondary peak around 280 nm thanks to aromatic amino acids like tryptophan and tyrosine (Figure 1B). ...

8. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

   Type

   Blog Post

   Published

   Oct. 4, 2018, 12:44 p.m.

   ... Addgene Blog Read this blog post about CRISPR Kinome Libraries available at Addgene Learn about the ...

9. Better Dyeing Through Chemistry & Small Molecule Fluorophores

   Type

   Blog Post

   Published

   Sept. 8, 2016, 2:30 p.m.

   ... (e.g., transcyclooctene–tetrazine)– Nonnatural amino acids can be incorporated into a protein structure...

10. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type

    Blog Post

    Published

    Aug. 9, 2016, 2:30 p.m.

    ...histone proteins K4 and K27 – locations of the amino acid lysine in H3 me1, me3, and ac – denoting ...

11. Tips for Screening with Yeast Two Hybrid Systems

    Type

    Blog Post

    Published

    Oct. 22, 2015, 2:30 p.m.

    ...El-Bakkoury, M., Hamacher, T., Cappellaro, C., Vilarino, C., Fleischer, C., Ellerbrok, H., Kamuzinzi, ...

12. Cpf1: A New Tool for CRISPR Genome Editing

    Type

    Blog Post

    Published

    Oct. 14, 2015, 2:30 p.m.

    ...two nucleases, AsCpf1 and LbCpf1 (1307 and 1228 amino acids long respectively), both cleave in a staggered...

13. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type

    Blog Post

    Published

    Oct. 6, 2015, 2:30 p.m.

    ... sample that is transformed that week is then inoculated for storage on Thursday. The lab prepares bacterial...

14. Your Lentiviral Plasmid FAQs Answered

    Type

    Blog Post

    Published

    April 23, 2014, 1:08 p.m.

    ...maximal biosafety available for the technology. The minor limitation in using the 3rd-generation system is...

15. Plasmids 101: Stringent Regulation of Replication

    Type

    Blog Post

    Published

    June 20, 2024, 1:15 p.m.

    ...Solar, G., Giraldo, R., Ruiz-Echevarría, M. J., Espinosa, M., & Díaz-Orejas, R. (1998). Replication and...

16. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type

    Blog Post

    Published

    Oct. 13, 2022, 1:45 p.m.

    ...introduce a single nucleotide modification at a defined amino acid to mimic a patient mutation or maybe to disrupt...

17. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type

    Blog Post

    Published

    Nov. 19, 2020, 2:15 p.m.

    ...small DNA binding motif in MECP2 interacts with the minor groove of AT-rich DNA sequences to regulate transcription...

18. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type

    Blog Post

    Published

    July 7, 2020, 1:15 p.m.

    ...the BFP mutant (BFPH66) contains a histidine at amino acid 66. This histidine is encoded by a ‘CAC’ codon...

19. QC Sequencing Technologies at Addgene

    Type

    Blog Post

    Published

    July 15, 2025, 1:15 p.m.

    ...theoretical sequences submitted to Addgene have minor differences due to in silico variations and lack...

20. Hot Plasmids: Fall 2024

    Type

    Blog Post

    Published

    Nov. 21, 2024, 1:30 p.m.

    ...variation on their previous sensor STReTCh, the 11-amino acid BiT fragment of luciferase is embedded in the...