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We narrowed to 663 results for: Mal

Showing: 201 - 220 of 663 results

  1. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...same cellular location, all at once! Small molecules What type of small molecules can you monitor with biosensors...thing that you care about within your cellular or animal system. This ‘thing’ could be a neurotransmitter... those which can be applied in cell culture and animal models primarily. Fun fact: Almost all of the biosensors...

  2. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...genetically engineered receptors to interact with small molecules to elicit a response in a certain cell...genetically modified receptors are activated by small molecules to either activate or inhibit neuronal...to respond to specific, pharmacologically-inert, small molecules. These new receptors were termed Designer...muscarinic receptor family and are activated by small molecule ligands such as clozapine-N-oxide (CNO)...

  3. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...to deliver Cas9 and your gRNA of interest into mammalian cells. This vector was the top requested plasmid...) V2.0 - This plasmid from the Zhang lab is a mammalian vector that expresses both SpCas9 and your gRNAs...scrambled shRNA is used as a negative control for mammalian RNAi experiments.  Find the scrambled shRNA vector...2A-GFP (PX461). pSpCas9(BB)-2A-Puro (PX459) - This mammalian expression vector encodes both SpCas9 and your...

  4. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    Published
    Nov. 5, 2019, 1:28 p.m.
    ...characterized by the Oakes and Doudna Labs, which is both small and potentially less likely to elicit a strong immune...also shows promise for in vivo research due to its small size and specificity relative to Cas9. As scientists...Teng, Fei, et al. "Repurposing CRISPR-Cas12b for mammalian genome engineering." Cell discovery 4.1 (2018)...Read our CRISPR guide Find CRISPR plasmids for mammalian systems ...

  5. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...long. The small size of the polyhistidine tag minimizes the chance of the tag disrupting normal protein ...function. In contrast, the polyhistidine tag is very small and rarely affects protein function (Bornhorst 2000...histidine-containing protein contamination, such as mammalian production systems, consider using these more ...

  6. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ... and have a diverse range of functions within mammalian cells. Here, we will cover how these structures.... Unlike dsRNA, DNA:RNA hybrids are abundant in normal cells and are arise from a ssRNA engaging one strand...putting DNA at risk. Consequences of duplexed RNA Mammalian cells have multiple receptors in place to sense...strands to re-pair. The two dominating RNases in mammalian cells (RNase H1 and H2) handle most of these intermediates...

  7. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type
    Blog Post
    Published
    May 11, 2021, 1:15 p.m.
    ... were in the cytoplasm of dendrites, and only a small proportion were found on the plasma membrane (top...neurons, most of them were still cytoplasmic. Use of ‘small’ tags to identify proteins Given the limited expression...plasma membrane. The haemagglutinin (HA) tag is a small 9 amino acid epitope of the haemagglutinin of the...mice and monkeys. Surprisingly, we found that this small change made a big difference. Replacing the mCherry...

  8. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...ways scientists communicate with each other: from formal mechanisms like publishing a research article, ...or submitting patent or grant applications; to informal settings like lab meetings, social media, coffee...before, submitting the manuscript to a journal for formal peer review. Some organizations, including some...preprints are eventually published in journals after formal peer review, with the earlier attention and feedback...

  9. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ... an autosomal dominant mutation in MYBPC3 that leads to hypertrophic cardiomyopathy. An adult male with...term ‘editing.’ While repair using endogenous chromosomal DNA is appealing for heterozygous mutations, ...they limited their whole genome sequencing to a small percentage of edited embryos. Since the embryos ...

  11. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ...). Anti-CRISPR (Acr) proteins are phage-derived small protein inhibitors of CRISPR-Cas systems that help... Acr proteins by inducible promoters, light, or small molecules could achieve rapid, dynamic, and spatial...cytotoxicity of CRISPR-Cas editing in tissues The small size of Acr proteins (~50-200 amino acids) means...is where Acr proteins come in. Acr proteins are small enough that they could be engineered to “armor” ...

  12. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    Published
    March 12, 2020, 1:15 p.m.
    ... neuron’s projections. Generating animal models: Transgenic animals allow for the expression of genes ...time-consuming to generate, especially for large animals such as primates. Viral vectors with cell-type ...to restrict expression of a gene interest in an animal model. A toolkit of synthetic AAV promoters The...

  13. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...challenging as they are great in number, generally very small, and can contain different neurotransmitters. Using...traditionally introduced in Caenorhabditis elegans on extrachromosomal arrays can lead to artifacts due to overexpression...used CRISPR with a ssODN template to introduce the small wrmScarlet11 which is less than 60 nt in length ...active in vitro and in human and plant cells. Its small size offers advantages for cellular delivery. Get...

  14. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...An estimated 320,000 viruses can infect mammals. Even more abundant are the Earth’s estimated 1031 bacteriophages...by resuspension of the collected particles in a smaller volume. Always remove packaging cell debris before...strategy to estimate unknown viral diversity in mammals." MBio 4.5 (2013): e00598-13. PubMed PMID: 24003179...events during integration of transfected DNA into normal human cells."Nucleic acids research 18.9 (1990)...

  15. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...homology-directed repair. Both Cpf1 and its guide RNAs are smaller than their SpCas9 counterparts, so they will be...in length, respectively, larger than SaCas9 but smaller than SpCas9. These nucleases should fit well into...the newly described non-CRISPR protein NgAgo is smaller than any of these options (2.7 kb with 24 nt guides...efficiency of two Cpf1 orthologs known to be active in mammalian cells, LbCpf1 and AsCpf1. In both reports, on ...

  16. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...types of RNA: Polymerase I is responsible for ribosomal RNA, Polymerase II is responsible for mRNA and...seems to couple termination with polyadenlyation. Mammalian expression plasmids are primarily used to create...create mRNA and the commonly used mammalian terminators (SV40, hGH, BGH, and rbGlob) include the sequence...commons.wikimedia.org/w/index.php?curid=17554393   Use of mammalian poly(A) signals in combination with certain viral...

  17. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...hundreds of stalls selling a myriad of items, only a small number of which I could identify. There were dozens...fragments of these transcripts. I remember when small RNA was just something we ran off the bottom of ... replication. He too mentioned how “surprising” small RNAs can be and posited that “we underestimate living...covalently closed RNAs seem to be conserved in mammals, expressed in neurons, and are especially abundant...

  18. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...paternal or maternal copy of the gene is expressed in normal individuals (in a parent-of-origin-specific manner...because too much or too little gene product disrupts normal function. Angelman syndrome is characterized by...both cases, the other copy of the chromosome is normally imprinted and silenced, and so the syndromes are...supply influenced cardiovascular mortality of the female grandchildren." BMC genetics15.1 (2014): 12. PubMed...

  19. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ...time (Figure 1). See the paper for more details on small differences between mGold2s and mGold2t depending...CRISPR has become a household name, the search for smaller genome editors is heating up and TnpB has found... an omega RNA, or ωRNA) (Figure 2), but TnpB is small enough to be carried by viral vectors (~400 amino...introduced heritable genome edits in a single step with minimal off-target effects. With this proof-of-concept,...

  20. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...of hundreds to tens of thousands of genes with minimal effort. The versatility of Cas effectors allows... the host genome. AAV instead relies mainly on episomal expression. Episomes are circular pieces of DNA... pool for downstream NGS and means you can use smaller volumes of your library for each experiment. Additional...to use variants of Cas9, like split Cas9, or a smaller Cas9 ortholog like SaCas9. You could even venture...
Showing: 201 - 220 of 663 results