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We narrowed to 313 results for: POR C

Showing: 201 - 220 of 313 results

  1. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    Published
    Oct. 31, 2019, 1:06 p.m.
    ...PubMed Central PMCID: PMC1559948. Brusca, Richard C., and Matthew R. Gilligan. "Tongue replacement in ... please! At Addgene, we love finding ways to incorporate science themes into our fun activities and Halloween...body. The fruiting bodies then rupture and release spores onto the forest below for the next unsuspecting...

  2. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    Published
    Feb. 18, 2015, 3:09 p.m.
    ...Bauer DE, Kamran SC, Lessard S, Xu J, Fujiwara Y, Lin C, et al. An erythroid enhancer of BCL11A subject to...work was with TALENs, in collaboration with the Porteus lab [2]. With the advent of CRISPR/Cas9, we began...correlative biochemical characteristics and ectopic reporter assays. We have been interested in exploring the...transfect CRISPR plasmids into MEL cells by electroporation, sort your transfected cells via FACS, primer...

  3. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    Published
    June 26, 2017, 1:31 p.m.
    ...Addgene.   References Ke, Q., Ahn, Y., & Sugimoto, C. R. (2017). A systematic identification and analysis...physical scientists are underrepresented. It’s important to note that these results could be biased for...domains more often than other scientists   Figure 5: Portion of Tweets with Scientific Domains, Categorized ...

  4. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    Published
    July 29, 2014, 6:16 p.m.
    ...attach the gene of interest to either the N-or the C-terminus. Even if a researcher’s gene of interest ...reagents. "We are proud to be able to bring such an important collection to the scientific community," says ...scientists, and the FSU team who have made this large, important plasmid deposit possible.   ...

  5. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...annurev-pharmtox-010814-124803 Vlasov, K., Van Dort, C. J., & Solt, K. (2018). Optogenetics and Chemogenetics...   Timing Optogenetics offers a high degree of temporal resolution as receptors can be turned on and off... meet these requirements. TL;DR If precise spatiotemporal control of a small subset of neurons is necessary...

  6. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ...proteins. Alternative splicing generates the different C-terminal regions of the Rep proteins. While the unique...capsid, “pumps” the genome into the capsid through a pore in the capsid. Rep proteins are also involved in...2020) Adeno-Associated Virus Genome Interactions Important for Vector Production and Transduction. Human ...

  7. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...transfection. This supernatant can be stored at 4 °C or can be directly added to your target cells. However...and plasmids.  References 1. Miller, A. DUSTY, and C. A. R. O. L. Buttimore. "Redesign of retrovirus packaging...or electrical (electric current forming membrane pores) transfection. These techniques are still used and...has been removed, Rev (which facilitates nuclear export) is expressed from a separate plasmid, and the ...you clone your gene of interest into and is an important feature for your experiment; it must be chosen...using them.   Regarding biosafety, it is also important to be aware that lentiviral particles are a powerful...

  8. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...PubMed Central PMCID: PMC4349094. 3. Bell, Charles C., et al. "A high-throughput screening strategy for... 22963480. PubMed Central. 5. Tsiatis, Athanasios C., et al. "Comparison of Sanger sequencing, pyrosequencing... the University of Cambridge. One of the most important steps in the CRISPR experimental process is validating...silico prediction. These techniques differ in important ways, but can also complement one another by providing... unbiased methods, high background BLESS Not reported Cell-based (in vitro) Can be used on tissue from...senstive to time since cell fixing HTGTS Not reported Cell-based Identifies translocations Limited by...

  9. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...neuronal phenotype in a mixed population (Burger, C. 2004, Aschauer, DF. 2013, O’Carroll, SJ. 2021). This...https://doi.org/10.1371/journal.pone.0076310 Burger C, Gorbatyuk OS, Velardo MJ, Peden CS, Williams P, Zolotukhin...fibrillary acidic protein (GFAP) for astrocytes it is important to test it in the brain region of interest, especially...of Transduction Efficiency, Tropism and Axonal Transport of AAV Serotypes 1, 2, 5, 6, 8 and 9 in the Mouse... the Addgene blog Plasmids 101: FLEx Vectors Important Considerations When Using AAVs Three Key Considerations...

  10. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    .... DOI: https://doi.org/10.1080/rsob.180241 Vanni, C., et al., Unifying the known and unknown microbial...fundamentally the reporter construct monitors the expression of a nearby gene. The reporter gene serves as... screens to introduce reporters throughout an organism’s genome. These reporters have historically been...stimulate the expression of the reporter. The system is made up of a reporter gene flanked by a minimal promoter...promoter – all rely on the random integration of a reporter construct in close proximity to an endogenous ...example, if green fluorescent protein (GFP) was the reporter gene and green cells were observed in the kidneys... for with an uncharacterized gene. The actual reporter construct can be something phenotypic (such as...

  11. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...of our most popular mammalian expression vectors, c-Flag pcDNA3, comes from the pcDNA series. It has the...consider as you make your choice. The first and most important thing you need to know is your expression system...have to make sure that your plasmid has been incorporated into the host organism, usually achieved with...basal expression level, induce expression at an opportune time or go all out with constitutive high level...Some of the more popular studies involve using a reporter vector to tag YGOI with lacZ and/or GFP (e.g. ...animals, gene targeting and genome modifications to reporter vectors (e.g. pBV-Luc) and gene silencing (e.g...

  12. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ... Deverman BE, Matho KS, Cetin A, Woodard K, Cepko C, Guerin KI, Rego MA, Ersing I, Bachle SM, Kamens J...’s ~4.4 kb or smaller. Some examples include a reporter like GFP, some smaller CRISPR-Cas proteins such...the Cre recombinase, are used to spatially and temporally control transgene expression. Lox sites are directional...sites lets researchers control the spatial and temporal activation of a transgene. Figure 2: FLEX...structure when transcribed which aids in nuclear export of mRNA and helps increase the expression of your...polyadenylation or polyA signal aids in the nuclear export of RNA and RNA translation, and promotes RNA transcript...

  13. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...Boeckman JX, Manning CF, Nguyen JT, Bechtold H, Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht...and have always been, a community resource that supports both toolmakers and tool users. We encourage you...Educational Resources As part of our commitment to supporting your research, Addgene is creating an array of...Through our incredible community, including your support, we have enabled scientists to easily share plasmids...Studies Hybridoma Bank, a nonprofit that has been supporting hybridoma sharing within the community for decades...Immunofluorescence Addgene’s Antibody Guide The project is supported by a NIH BRAIN Initiative grant (U24NS119916 )...

  14. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ...∆G, however, can not be conjugated to PE due to a C-terminal glycine deletion, so it remains in the cytosol... development of several fluorescent autophagy reporters now allows for the measurement of autophagic flux...makes Keima useful for monitoring mitophagy. It’s important to remember that because Keima’s fluorescent relies... al. "Rosella: a fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in...using a single fluorescent biosensor." Scientific reports 5 (2015): 12397. PubMed PMID: 26215030. PubMed ...

  15. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    Published
    Sept. 21, 2023, 1:15 p.m.
    ... antibodies aren’t free to bind endogenous IgGs. (C) A third solution is to use Fab fragments against ...primary antibody that is directly conjugated to your reporter circumvents the issue. However, directly conjugated...which uses just a primary antibody that has your reporter (in this case a fluorophore) directly attached... as effective for other techniques, but it is important to be aware that the issue is not unique to IHC...

  16. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...) antibody-dependent cellular cytotoxicity (ADCC) c) antibody-dependent cellular phagocytosis (ADCP). ...starting a cascade of events that results in the pores being opened in the pathogen’s cell membrane (Dunkelberger... when holes are punched in their membranes, the pore'd pathogens quickly lyse and die.  Antibody dependent...

  17. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...under the United States Internal Revenue Code 501(c)(3). That means we were formed to benefit the public...do. From all of us to all of you. Thanks for supporting nonprofit, open science and Addgene in our mission...Collaboration with Fellow Nonprofit Seeding Labs Addgene Supports Rare Disease Research Listen to Our Interview ...

  18. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...released from nuclei by a short incubation at 37 °C. These short DNA fragments can then be purified for...MNase to antibody bound proteins (Figure 1). Importantly, since Protein A has weaker affinity for mouse... rather than to rely on nuclei extraction. They report that this change offers a significant improvement...

  19. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...receptors. Scientific Reports, 10(1). https://doi.org/10.1038/s41598-020-78827-9 Breen, C. J., Raverdeau, M...Muthusamy, A. K., Liang, Y., Brown, T. A., Lemon, W. C., Patel, R., Lu, R., Macklin, J. J., Keller, P. J....protein function and cellular components: It's important to assess how dye-conjugation affects protein ... interpretation of experimental results. It's important to consider how dyes can influence receptor conformation...efficacy of these ligands can be affected by the incorporation of fluorophores, as seen in studies involving...wavelengths for green, red, and far-red dyes is important for intracellular fluorescence live imaging ​​(...from cells under certain conditions, which is an important consideration for assay design (Breen et al., ...

  20. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...T-regs) into their microenvironment by producing C-C motif chemokine 22 (CCL22) and TGFβ. T-regs are suppressive...still many important questions in this exciting field of research. One of the most important: How will ...commute to and invade multiple organs, and most importantly escape the immune mechanisms of the host. The...against cancer. Sipuleucel-T by the Dendreon Corporation is currently the only FDA-approved DC therapy...therapies themselves can be made more effective. Some important factors researchers are beginning to focus on ... of cell types. This cell heterogeneity is an important element of successful tumor progression. A tumor...), and ability to metastasize. There have been reports of clinical trials going amiss and patient deaths...
Showing: 201 - 220 of 313 results