We narrowed to 611 results for: REV;

...Editing Technology at Beam Therapeutics, used a previously developed bacterial selection strategy (Gaudelli...more efficient. Informed by these mutations and previously described TadA mutations (Gaudelli et al., 2017...mitigated with the expression of fetal hemoglobin. Previous studies have shown that one base change in the...

...dust since the digital music revolution hit. I predict a similar revolution for antibodies. Recombinant...expression of the mAb-encoding genes or fail to revive after cryopreservation (Bradbury & Plückthun, 2015a...

...symposia held across the USA during this time have revealed that young scientists are aware of, and are engaged...Academy of Sciences. “The Postdoctoral Experience Revisited” 2014 Appendix B p93-95). For those advocating...25653845. Pubmed Central PMCID: PMC4304227. 4. Revitalizing biomedical research: recommendations from the...

...a table. It adds a formality and distance that prevents interaction with each other and the audience. ...moderator takes control It is the moderator's job to prevent anyone from derailing the show. The moderator must... active at all times keep things moving and to prevent any one panelist or audience member from taking...

...for dicots. NC: nucleocapsid RNA chaperone, RT: reverse transcriptase. Right: T1 tomato plants that inherited...genetically-encoded voltage indicator (GEVI) than previous versions. ASAP5 can be imaged using one- or two-photon...across many cell types in varying conditions, revealing context-dependent responses and molecular patterns...

...editor (typically a Cas9 H840A nickase fused to a reverse transcriptase) together with a prime editing guide...sequence containing a primer binding site and a reverse transcription template (RTT). The design of the...and highly versatile genome manipulation. Nature Reviews Genetics, 24(3), Article 3. https://doi.org/10.1038...

... series from Subach et al.) were derived from previously developed monomeric fluorescent proteins mKO ...dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer.... German. "Chronology of islet differentiation revealed by temporal cell labeling." Diabetes 58.8 (2009...

...Inspired Engineering. CRISPR/Cas9 technology has revolutionized the fields of molecular biology and bioengineering...which variant of Cas9. To circumvent this problem, previous studies have proposed the concurrent use of “orthogonal...gene of interest, Cas9 forms a steric block that prevents RNA polymerase from efficiently transcribing the...

...is suitable for your application. We have previously reviewed many common lab E. coli strains on the blog...optimal growth, as insufficient gas exchange will prevent your cultures from reaching the desired density...

... to repair DNA. The DNA ends are chewed back to reveal homology, allowing the strands to anneal. DNA synthesis...Using MMEJ for gene knock-in Building on the lab’s previous work, Sakuma et al. describe a detailed protocol... using much shorter sequence homologies. Abbreviated protocol for PITCh Step 1: Generate microhomologies...

... for granted. In this post, we take a moment to review what exactly you are measuring and how to interpret...starting from the beginning. Before trying again, revisit your purification protocol. Are any of your materials...and quality than we have covered here! Be sure to review resources on the specific instrument you use and...

...tethered to a repressive domain, such as KRAB, to prevent efficient transcription from sgRNA targets. CRISPRi...gain of function screens. These screens can help reveal the function of lowly expressed genes or genes ... sgRNAs were selected and ranked based on the previously established sgRNA rules and  potential on- and...

...more about how nanobodies are identified in this review. If you just want to use the nanobodies described...picking a secondary nanobody, it’s important to review a few characteristics of your primary antibody:...Nanobodies: natural single-domain antibodies.” Annual review of biochemistry 82 (2013): 775-97. PubMed PMID:...

...inhibitors are tested in parallel for their ability to prevent BiFC resulting from the assayed PPI. This high-...However, it’s important to note that BiFC is irreversible — FP fragments become covalently bonded by associating...analysis of protein homomerization in Saccharomyces cerevisiae. Genome Res 29:135–145. https://doi.org/10.1101...

...experience. Along with the updated search, we’ve also revamped our homepage and mascot Blugene (check back on...search terms and potential results. Just as in the previous layout, the number of plasmids matching each filter...the source organism for a gene insert. (e.g. S. cerevisiae) Plasmid Type: Choose an empty backbone if you...

...way to improve the photon budget. Of course, “reverting” to small molecule dyes seems daunting—no one ...enzyme variant tag that reacts specifically and irreversibly with a small substrate ligand motif attached...strategies, several groups—including mine—have been revisiting the old chemistry of dyes. The first synthetic...

... both favors antibiotic sensitive bacteria and prevents sensitive bacteria from acquiring resistance genes...settings where antibiotic resistant bacteria have previously flourished.   Figure 2: Two-phage strategies...Infection with the lytic phage kills bacteria not previously infected by the lysogenic phage, rendering the...

...hybrid systems were developed in Saccharomyces cerevisiae in 1989 and are still used extensively to screen...heterologous systems (e.g. mammalian proteins in S. cerevisiae). While this may be difficult to resolve for...Kamuzinzi, R., Ledent, V., Blaudez, D., Sanders, D., Revuelta, J.L., Boles, E., André, D., and Frommer, W.B....

...itself to be quite the mental puzzle if you lack previous experience with it. Don’t worry, we’ve got you...to be in the same (forward) or in the opposite (reverse) orientation? The answers to questions like these...plasmid map and identify any mismatches that are preventing your overhangs from aligning properly. Do this...

...antibodies to simian virus 5 and their use in revealing antigenic differences between human, canine and... et al., 2024). In this new variation on their previous sensor STReTCh, the 11-amino acid BiT fragment...excitingly, assembly of the split luciferase is reversible, making PILATeS suitable for live imaging with...