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We narrowed to 488 results for: Ret;

Showing: 201 - 220 of 488 results

  2. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ... great idea to most people in 2005, but XKCD is pretty successful!  What resources do you have?  No matter...for children” or may need text for audience interpretation. Infographics Low Combines both words and...

  3. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...within the target window, possibly due to longer stretches of duplexed RNA. Using an ADAR2DD catalytic mutant...perform C-to-U edits, the authors note that it retains its adenine deaminase ability. The authors then...

  4. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...Komagataella phaffii (a.k.a  Pichia pastoris) because it secretes fewer of its own proteins, making the purification...production of Fab fragments where variable chains are secreted into the periplasmic space to be oxidized and ...

  5. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...proteins were successfully expressed as fusions that retain their individual activities (1). Researchers also...DBD) and transcriptional activation (TA) domains retain their individual activities when separated (2),...

  6. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...grow. As the bacteria divide, they replicate and retain the plasmid. This allows us to make unlimited copies...deposit. Using barcoded tubes is part of Addgene’s secret to success. It helps us quickly process samples...

  7. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...cell’s genome, so no special sequence is needed to retain the vector as cells divide. In transient transfection... and eukaryotes. Conclusion I still think it’s pretty incredible that my cells “speak” the same molecular...

  8. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...trainee (even if you’re an oldbe trainee!) can be pretty nerve-wracking. So how can you be the best trainee...gives twofold benefits: you may learn faster and retain more information plus your mentor will get you ...

  9. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...typically contains one viral genome. Other than a theoretical maximum value, physical titers don’t necessarily...different sources in the same experiment, consider retitering both AAV in your lab. The absolute titer values...

  10. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...antibodies. In contrast, with their many epitopes, smFPs retain their ability to be detected after sample preparation...the Addgene Blog Rabies and neuronal tracing Retrograde AAV: Making the journey from axon to nucleus ...

  11. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...kill your productivity. Harvard Business Review. Retrieved January 14, 2022, from https://hbr.org/2019/11...innovation on your team. Harvard Business Review. Retrieved January 14, 2022, from https://hbr.org/2019/07...

  12. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...exported to the periplasm of bacteria where it can be secreted into the supernatant. Four plasmids containing...in human pluripotent stem cells(hPSCs), Lindy Barrett’s lab at the Broad Institute developed fluorescently...

  13. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...known 'DNA adenosine deaminases' which would theoretically give rise to an adenine base editor. A massive...developed using the hAPOBEC3 deaminase can edit in a stretched window ranging from 1-17 bp relative to the PAM...

  14. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...pCAG-CreERT2 #14797). These Cre/ FLP versions are retained in the cytoplasm until estrogen receptor ligand...FLEX-vectors Learn More about Cre-Lox Check out Retrograde AAVs for Neurscience Research Resources on Addgene.org...

  15. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...faculty, students, private sector researchers, or pretty much anyone applying the scientific method to a..., also known as the science videographer, is a retired botanist and oceanographer who has a blog and several...

  16. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ..., A., Garnier, A. “Downstream processing of oncoretroviral and lentiviral gene therapy vectors.” Biotechnology...conditions on the production of lentiviral and retroviral vectors.” Human Gene Therapy Clinical Development...

  17. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...salts may be preferable if the protein product is secreted to the medium or if slow expression is required...required due to solubility concerns. Insoluble and Secreted Proteins: The most common purification protocols...

  18. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...expressed constitutively, either in lentiviral or retroviral backbones. On some occasions, shRNAs are transiently...%}   Interfering in the future While RNAi has retreated a bit to the background, it remains a solid option...

  19. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...include the quality control of secretory proteins in the Endoplasmic Reticulum, optimization of fluorescent...

  20. Transferable Skills Guide: Time Management

    Type
    Blog Post
    Published
    Dec. 5, 2017, 1:52 p.m.
    ...entire experiment, but you’ll have to spend the entirety of each day doing the experiment. The second way...during my day according to my priorities, I have a pretty good idea about the specific things I’ll be doing...
Showing: 201 - 220 of 488 results