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We narrowed to 743 results for: TRI

Showing: 201 - 220 of 743 results

  1. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...dependencies they identified and their associated ancestries. While there were some exciting relationships...Dependency Map) than lines from European or East Asian ancestries. These SNPs, however, were not accounted for ...racial bias. It is easy, even unintentionally, to attribute to an ancestry group what is actually an artifact...and references References Germline variation contributes to false negatives in CRISPR-based experiments...experiments with varying burden across ancestries. Sean A Misek, Aaron Fultineer, Jeremie Kalfon, Javad Noorbakhsh...

  2. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...generally referred to as Antigen Retrieval. I chose not to get into antigen retrieval here because it’s not quite...permeabilization reagents are detergents, such as Triton X-100, Tween-20, or saponin. Each detergent has...membrane-associated proteins in place. In contrast, Triton X-100 and Tween-20 are examples of non-ionic detergents...Permeabilizing with a non-ionic detergent, like Triton X-100, can lose membrane-associated proteins, but...

  3. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...It is also worth noting that plasmids can be symmetrically incompatible, meaning that both plasmids are... from the cell with the same probability, or asymmetrical, where one plasmid lost from the cell at a higher... higher rate than the coresident plasmid. Asymmetric plasmid loss occurs for several reasons, including...high copy incompatible plasmids that lead to asymmetrical plasmid loss of the usually large, low copy ...: 15916596 Kamruzzaman M, Shoma S, Thomas CM, Partridge SR, Iredell JR (2017) Plasmid interference for...

  4. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type
    Blog Post
    Published
    Dec. 14, 2017, 2:08 p.m.
    ...This post was contributed by Kusumika (Kushi) Mukherjee, a Postdoc at Massachussetts General Hospital...patients with skin disorders for research and clinical trials, as it allows access to a larger number of willing...phenomenon has been recorded in past gene therapy trials and remains a safety concern in the clinical field...Furthermore, working with Sendai virus requires stringent biosafety measures, thereby increasing the costs...Using Serial Plating of Reprogrammed Cells by Centrifugation. Stem Cells Int, 2016. 2016: p. 1329459. PubMed...

  5. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ... Weakly electric fish are excellent systems to study for convergent evolution because electric organs ...leave them to their own devices. Chytrid fungi (Lillian Fritz-Laylin) Chytrid fungi are useful for the study...but there are few genetic tools currently for terrestrial frogs. Cnidarian-Dinoflagellate symbionts (Virginia...

  6. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...controlled by collections of neurons that are intricately wired into circuits through synaptic connections...weakly expressed. As these epitopes tags have no intrinsic visual signal, they cannot be detected directly...neurons. As compared to epitopes tags, FPs are intrinsically fluorescent and can therefore be used in live... interactions. Also, overexpression of FPs can trigger protein aggregation which is toxic for the cells...darkened chromophores. These probes are both intrinsically fluorescent and can be detected with highly ...

  7. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    Published
    Aug. 13, 2019, 12:58 p.m.
    ...This post was contributed by Katherine Rogers, a postdoctoral researcher at the Friedrich Miescher Lab...processes—not just development—are available from strain distribution centers in the US, Europe, and China. In addition...pathways—such as those regulated by one-eyed pinhead—contribute to the “sculpting” of tissues and organs during...Katherine W. Rogers is a postdoctoral researcher in Patrick Müller’s group at the FriedrichMiescher Laboratory...El-Brolosy, Mohamed A., et al. "Genetic compensation triggered by mutant mRNA degradation." Nature 568.7751 (...

  8. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ... This post was contributed by Ryan Watkins, Professor at George Washington University and developer of...may also benefit, such as researchers in other countries or ot­her disciplines, are even less likely to...research from other disciplines, or from other countries, to explore new connections to our research. So...hours of video were watched each month, in 39 countries, and in over 54 languages. While most scientific...citation rates to measure the impact of their contribution. It offers a 5-step process and several examples...

  9. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...This post was contributed by ATCC Outreach Scientist Nick Amiss. Cell culture in the present day Cell...major cell lines on public databases. Image attributions (from top to bottom) Database Center for Life...culture outlined in this article. Now many journals strictly require evidence of cell line provenance and identity...Nature Publishing Group recently reiterated its strict stance on the matter. Funding bodies such as the... at LGC Group, London, which is the European distributor for ATCC. He studied biochemistry at Warwick ...

  10. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...This post was contributed by guest blogger, Stephanie Hays, a scientist with a passion for photosynthetic...States government? Politics and science are intrinsically linked: governments exist in reality and science...said, government must have the same fundamental attribute that enables science: “humility in the face of...supporting many different positions. It would be detrimental for them to be homogenized or thought of as just...Scott Pruitt says carbon dioxide is not 'primary contributor' to global warming The New York Times: With Trump...

  11. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...DNA, important when trying to cut with certain restriction enzymes (ex: ClaI or XbaI) dcm DNA cytosine...DNA, important when trying to cut with certain restriction enzymes that are methylation sensitive. dnaJ...relA1 Relaxed phenotype, mutation eliminating stringent factor Allows RNA synthesis in absence of protein...plasmid maintenance, blue/white screening, partly restriction-deficient; good strain for cloning repetitive...methylated, allows for methylation sensitive restriction enzymes to cut the plasmid after preparation....

  12. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    Published
    Feb. 4, 2015, 3:58 p.m.
    ...labs have deposited plasmids to Addgene and we distribute over 130,000 plasmids in 2014. Thus, we are in...on CRISPR technology, for example, the authors Patrick Hsu, Eric Lander, and Feng Zhang acknowledge the...accelerated through a combination of open-source distributors such as Addgene, as well as a number of online...with data comparing Addgene global deposit and distribution trends for Zinc Finger, TALE, CRISPR, and SynBio...

  13. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...announce that we recently acquired the ability to distribute plasmids with the piggyBac™ transposon. These...piggyBac™ transposase (available from a licensed distributor) allow you to quickly transfer a DNA sequence...transposon vector to one of many TTAA sequences distributed throughout the genome. We encourage you to deposit...expand this useful resource. While Addgene cannot distribute plasmids with the piggyBac™ transposase itself...from the folks at Transposagen. This post was contributed by Amar Singh and Mathew Goodwin from Transposagen...

  14. Our New Antibody Mascot is....

    Type
    Blog Post
    Published
    Feb. 24, 2022, 2:15 p.m.
    ...preparation for our upcoming affinity reagent distribution launch - and we're excited to say our long naming...to help us with our upcoming affinity reagent distribution launch! There was only one problem - we didn't... biggest discovery yet: Addgene will soon be distributing affinity reagents. Name Our New Antibody Friend...luck and happy naming!   Fig. 2: The mascot trio!     ...

  15. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...This post was contributed by Kartik Lakshmi Rallapalli, a graduate student at the University of California...manipulate it. The term genetic engineering itself is attributed to SciFi writer Jack Williamson who used it in... Zinc finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding...template.  Each zinc finger recognizes a nucleotide triplet and when several ZFNs are strung together, it can...the ZFNs, but instead of recognizing nucleotide triplets, the components recognize individual nucleotides...

  16. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...This post was contributed by guest blogger Eleanor Wang, a Research Specialist in Patrick Hsu's lab at ...problems. Biomedical research, for instance, contributes heavily to medical practice, and understanding... and applied. The current pandemic serves as a striking example of the applicability and importance of...Eleanor Wang is currently a Research Specialist in Patrick Hsu’s lab at UC Berkeley. She will be starting ...

  17. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...This post was contributed by guest blogger Søren Hough, a Biochemistry PhD candidate at the University...mismatch along the length of the gRNA given the stricter sequence requirement at the terminal 3’ PAM site... Dillard, and Ayokunmi Ajetunmobi for their contributions to this work. Søren Hough is a Biochemistry ... mismatch cleavage assays to detect mutations triggered by engineered nucleases." G3: Genes| Genomes| ...PMID: 27087594. 16. Tycko, Josh, Vic E. Myer, and Patrick D. Hsu. "Methods for Optimizing CRISPR-Cas9 Genome...

  18. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    Published
    March 26, 2015, 3:24 p.m.
    ...scientists at 5,200 institutions in 82 different countries. We continue to ship out more plasmids each year...beginnings in 2004 to today, you can see how plasmid distribution has grown. Additionally, more and more scientists...research. *2015 total reflects the number of distributed plasmids as of March 26, 2015. How does Addgene... our depositors to allow us to help store and distribute their plasmids. The partnerships and collaborations...

  19. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...SaCas9 genome editing plasmids String assembly gRNA cloning (STAgR) String assembly gRNA cloning (STAgR)...studied Cas12b nuclease from Alicyclobacillus acidoterrestris (AacCas12b) exhibits optimal DNA cleavage at...STAgR), developed by Stefan Stricker’s lab, is a single step gRNA multiplexing system that generates vectors...

  20. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    Published
    Jan. 23, 2014, 3:23 p.m.
    ...component of Golden Gate and GreenGate are type IIS restriction endonucleases. They are like other endonucleases... should in principle work in any plant species. Tricks of the trade Lohmann does have one special tip ...their own lab: be very careful with the type IIS restriction endonuclease of choice, BsaI, which can be very... will get busy with GreenGate, producing and contributing more modules into the public domain in the process...
Showing: 201 - 220 of 743 results