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...distinguish between DNA and RNA. See our protocol about using UV absorbance to quantify DNA Fluorescence...absorbance based methods, this method doesn’t tell you about contaminating proteins or chaotropic salts. Capillary... instruments are available. Some last reminders about quantifying DNA In choosing your DNA quantification...Additional resources on the Addgene blog Read more about molecular biology protocols and tips Find the best...best way to elute and store your plasmid DNA Learn about the different grades of plasmid DNA preps Resources...

...frequency of use in black. This paper talks more about the development of the De-jargonizer.   Academic...with others which makes it easier to work with collaborators. Zotero is a desktop based program that’s compatible...episode 2 "Organizing Literature" : I found out about Zotero here Dear Grad Student podcast episode 2 ...Programming, & Productivity": This podcast talks about Mendeley, Zotero, and other citation managers and...the pomodoro technique": This podcast talks more about using the Pomodoro method in grad school Additional...

...Bachle and Ina Ersing Seeding Labs and Addgene collaborate to provide scientific resources, equipment, and...discovery, and synthetic biology. Read on to learn more about the five awardees and their promising research projects... to the Addgene blog for updates and future collaborations. Many thanks to all of the awardees, all who...Additional Resources on the Addgene Blog Learn More About the Addgene-Seeding Labs Plasmid Grant Check Out...Expression Systems Synthetic Biology Resources Learn about Global Plasmid Distribution ...

...resources on the Addgene blog Learn about company culture at Addgene Read about other lunch activities at Addgene...scientists bring biases to research and don't collaborate with others to bring diverse perspectives. Everyone...Science is a terrifying and equally good wake up call about the science of race."     - Joanne Kamens, Executive...easy reads, some are hard. Some I’ll never think about again, some I loved so much I’ll force them on everyone...

... and we thought it would be a good time to talk about the amplification and verification processes we ... DNA. These reactions should allow us to obtain about 100 to 1,000 bacterial colonies per individual plasmid...from the count_spacers.py file from Feng Zhang’s laboratory. There are a number of other analysis scripts...you have any tips, input, or stories of interest about library use, let us know in the comments! Additional...NGS Sequencing to Validate AAV Preps Learn More about Pooled CRISPR Libraries Resources on Addgene.org...

...during the day? We asked three lab heads how they go about selecting new hires. This is followed by some perhaps...visits the lab for a full day, gives a 1 hour talk about his or her interests, and spends one-on-one time...current members to share short-term goals and chat about awesome science. Important factors in the search..., the lab has a reputation for not entering collaborations. Alternatively, perhaps the lab publishes and...Medical School) for taking the time to speak with us about adding new scientists to their labs. Read the Other...

...reason behind this isn’t really hard to guess. Think about it like winter tires in different conditions: the...stable folding and efficient functioning. But what about placing the mesophilic hosts at 0°C? That won’t ...them which help foster a more productive and collaborative environment for research.  We’ve also involved...understanding of the societal ramifications of laboratory science, and how it may best be leveraged for...re always delighted to engage with potential collaborators and interested audiences. Please visit our team...

... Learn more about research applications for antibodies in Intro to Antibodies Read about the Developmental...antibodies in your experiments, you may be curious about all of the different kinds of antibodies that are...Disadvantages of monoclonal antibodies As mentioned above, genetic drift is one concern when using hybridomas...Hybridoma Bank, a repository for hybridomas  Learn about monoclonal antibodies for immunotherapy Resource...

...often relies on antibiotic resistance. But what about identifying clones that contain your desired insert... restriction digest. The key is to be strategic about your enzyme choices. You’ll want to pick enzymes... for your PCR.  Find the details and learn more about colony PCR in this blog post.   Figure 2: ...Sanger sequencing or the other techniques mentioned above. In molecular biology, cloning a gene into your ... and tips Resources on Addgene.org Learn more about Sanger sequencing Read the Molecular Biology Reference...

...We also want to tell you about things you didn’t know you wanted to know about, like research breakthroughs... want to know more about! You can also suggest blog topics or even reach out about writing a guest post...technical skills, and experiences from thousands of laboratories that makes up our Help Center, blog, website...

...Cancer, Inflammation and Immunity and learn more about “... recent findings in basic immunology, cancer-immune...curing cancer in cross-disciplinary projects and collaborations. Summarized by Professor Michael Karin from...This was followed by a talk from Professor Karin about alcohol and obesity as driving factors for chronic...got a chance to talk with Dr. Friedmann-Morvinski about tools used to analyse the tumor microenvironment...community, contact [email protected] to find out more about our free deposit and distribution service! References...

...in Huntsville explains it, a major goal in his laboratory is to understand the function of the non-coding...protein tagging. We spoke with Mendenhall and Myers about their new CRISPR tagging system, which they’ve now... antibody against them. There’s also a question about how specific those interactions are. This is solved...This design cuts out weeks of waiting and hands on labor. That’s a huge deal. With Addgene, now these can...system or who use and adapt our reagents to think about adding them to Addgene’s special collection of CRISPR...

...to understand the basis for cardiovascular and metabolic human diseases. They do that by studying patients...disease-associated genes. Addgene spoke to Musunuru about how the new kit works, the research the kit now ...number of genes involved in cardiovascular and metabolic diseases. We are knocking out genes that have ...to examine the effect of a gene on cholesterol metabolism. The TALEN kit is quite useful in generating ...disease modeling in stem cells. Addgene: Tell me about the history. How had investigators done this kind...

...include a note about where you met IRL (in real life) and perhaps what you talked about (or how good the... this rule, but not that many). I’m asked a lot about requesting connections to complete strangers for...have been able to tell when some start-ups were about to be bought by seeing certain groups of people ...using social media as a scientists Read blog posts about networking ...

...information about the contents of the autophagosome nor does it provide information about what is being...higher pH of the vacuole. Read on to learn more about prior methods for studying autophagy and how Rosella... (as opposed to giving more fine-grained detail about what proteins or organelles are being degraded) ...autophagy, but, like the protein degradation assay above, it’s slow and only measures bulk autophagy. Morphological...

... data and the list goes on and on. As you think about your career (and your future in general), keep in...in this post benefit from the applicant thinking about the process early, keeping good records, and planning...writing, compliance, core facility management, collaboration management, grant administration, clinical operations... in professional publications written by others about the alien's work in the academic field Evidence ...people who have not worked with you directly or collaborated on a project where they provided you with feedback...Celano, CEO Propel Careers. Lauren is passionate about working with scientific individuals to find exciting...

...different SARS-CoV-2 spike variants. You can learn more about their research in this article. To see other plasmids... optimized. Browse the collection or learn more about it on the blog. COVID-19 research is happening fast...through plasmid sharing and more. As you’ve read above, some of these plasmids have been in the repository...Additional resources on the Addgene blog Learn more about mapping the SARS-CoV-2 - human protein-protein interaction... interaction Find tips for remote collaborations Browse all COVID-19 blog posts Resources on Addgene....

... posters can be. You have also probably thought about why certain posters resonate with you (or not). ...important to reflect on these impressions when thinking about crafting your own poster. What sets a scientific...Making your poster readable While we are talking about font, remember this: bigger is better! Your poster...gradient (as is shown in all of our illustrations above) so that the color fades to a lighter or darker ...conference attendance Read all of our blog posts about conferences ...

...resources on the Addgene blog Learn about RNP delivery Learn about CRISPR delivery methods for mammalian...Nanoblades pseudotyped with a mix of VSV-G and the baboon endogenous retrovirus Rless glycoprotein (BaEVRless...have lower off-target edits. Another great thing about Nanoblades? They’re easily produced in a BSL2 lab... guide Find CRISPR plasmids and resources Learn about viral production ...

...Have you ever been stuck googling about an antibody that will be essential to your new research project...high-quality antibodies for IHC. Working with collaborators and support from the Brain Research Through ...extensive library of monoclonal antibodies; Addgene collaborates with these companies to create and provide general...pattern should align with what the atlases (mentioned above) and literature suggest, providing an expectation...open-source database, the mAb3D Atlas will foster collaboration, progress, and innovation by allowing scientists...