We narrowed to 235 results for: bon;

1. Viral Vectors 101: Preparing Pooled Libraries

   Type

   Blog Post

   Published

   July 13, 2023, 1:15 p.m.

   ...with a protocol for a library of similar size and backbone.  Verification Once your library has been amplified...

2. Plasmids 101: Stringent Regulation of Replication

   Type

   Blog Post

   Published

   June 20, 2024, 1:15 p.m.

   ...BioRender.com. Once DnaA breaks the local hydrogen bonds holding the DNA together, it recruits helicases ...

3. Viral Vectors 101: Pseudotyping

   Type

   Blog Post

   Published

   April 20, 2021, 2:30 p.m.

   ... type, but other envelopes such as the one from gibbon ape leukemia virus (GALV) are more stable (Sandrin...

4. CRISPR-based Genome Editing Systems For Editing in E. coli

   Type

   Blog Post

   Published

   April 13, 2021, 1:15 p.m.

   ...oligos are required and the BsaI linearized pEcgRNA backbone can be prepared in large amounts and frozen for...

5. Finding nucleic acids with SHERLOCK and DETECTR

   Type

   Blog Post

   Published

   April 16, 2020, 1 p.m.

   ...O, Jinek M (2016) Structural basis for the endoribonuclease activity of the type III-A CRISPR-associated...

6. Using Video to Share Your Science: We Share Science

   Type

   Blog Post

   Published

   Nov. 3, 2016, 2:30 p.m.

   ...rather than on the day-to-day research that is the backbone of science. Facebook is continuing its growth,...

7. Tips for CRISPR Gene Editing in Mice

   Type

   Blog Post

   Published

   June 28, 2016, 10:59 a.m.

   ...for validating your selected gRNA’s, and has the bonus benefit of supplying you with primers for validating...

8. Plasmids 101: The Promoter Region – Let's Go!

   Type

   Blog Post

   Published

   April 3, 2014, 8:05 p.m.

   ...means that the promoter present in your plasmid backbone must to be compatible with the type of RNA that...

9. The Advances Behind The World's First Personalized CRISPR Treatment

   Type

   Blog Post

   Published

   Sept. 16, 2025, 1:15 p.m.

   ...did not create double-stranded breaks in the DNA backbone, making them significantly less prone to indel...

10. Fluorescent Proteins 101: When GFP lets you down

    Type

    Blog Post

    Published

    Aug. 23, 2018, 12:05 p.m.

    ...the Fluorescent Protein Collection Find empty backbones for fluorescent protein fusions Browse the Fluorescent...

11. Adeno-associated Viruses (AAVs) for Genome Editing

    Type

    Blog Post

    Published

    March 27, 2018, 1:32 p.m.

    ...devised methods to deliver CRISPR components as ribonucleoproteins (RNPs) that can be supplied separately from...

12. Harnessing Bacterial Toxins for Allelic Exchange

    Type

    Blog Post

    Published

    Aug. 15, 2019, 12:30 p.m.

    ...the desired mutation and excision of the vector backbone. This step requires “counter-selection,” meaning...

13. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type

    Blog Post

    Published

    April 9, 2019, 1:13 p.m.

    ...the fluorescent protein collection Find empty backbones for fluorescent protein fusions Browse the fluorescent...

14. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type

    Blog Post

    Published

    Sept. 5, 2017, 1:58 p.m.

    ...How to Design Your gRNA Delivering CRISPR Ribonucleoproteins Resources on Addgene.org Browse All CRISPR...

15. March for Science

    Type

    Blog Post

    Published

    April 21, 2017, 2:30 p.m.

    ...Scientists' Views PolitiFact: EPA head Scott Pruitt says carbon dioxide is not 'primary contributor' to global ...

16. CRISPR 101: Homology Directed Repair

    Type

    Blog Post

    Published

    Jan. 26, 2023, 2:15 p.m.

    ...alleles using long ssDNA donors and CRISPR ribonucleoproteins. Quadros RM, et al. Genome Biol. 18(1); (...

17. The Fluorescent Vegetables in Aptamer Soup

    Type

    Blog Post

    Published

    Jan. 29, 2019, 2:27 p.m.

    ... Panchapakesan, Shanker Shyam S., et al. "Ribonucleoprotein purification and characterization using RNA...

18. Multiple Plasmids at a Low Price: Kits from Addgene

    Type

    Blog Post

    Published

    Sept. 14, 2016, 2:30 p.m.

    ...create the pCell-Free vectors, Gateway-compatible backbones that enable cell-free expression of proteins in...

19. 10 Steps to a Perfect Science Talk

    Type

    Blog Post

    Published

    Aug. 23, 2016, 2:30 p.m.

    ...hiring manager or host for topic advice. An added bonus to knowing your audience is that it can help you...

20. Is this the right place for me? 8 tactics for choosing a lab

    Type

    Blog Post

    Published

    Oct. 2, 2018, 12:56 p.m.

    ...still think “but that won’t happen to me, I have a backbone”. The sad truth is, if it happened before, it ...