We narrowed to 266 results for: iNOS
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TypeBlog PostPublishedJune 18, 2020, 1:15 p.m....different trinucleotides (corresponding to different amino acids) in the regions that bind to the antigen. ...
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Tips for Using BLAST to Verify Plasmids
TypeBlog PostPublishedMay 29, 2014, 1:29 p.m....blastx results start the alignment at a more distal amino acid. We consistently run our blastx searches with... -
What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry
TypeBlog PostPublishedJuly 1, 2025, 1:15 p.m....secondary peak around 280 nm thanks to aromatic amino acids like tryptophan and tyrosine (Figure 1B). ... -
Antibodies 101: Producing Recombinant Antibodies
TypeBlog PostPublishedAug. 13, 2024, 1:15 p.m.... though this can vary depending on the specific amino acid sequence (Maity et al., 2015). If your antibody... -
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog PostPublishedOct. 4, 2018, 12:44 p.m.... Addgene Blog Read this blog post about CRISPR Kinome Libraries available at Addgene Learn about the ... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog PostPublishedJuly 30, 2020, 1:15 p.m.... Aliyah Weinstein, Fabienne Furt, and Quintin Marcelino for their helpful discussion of this book.... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog PostPublishedAug. 30, 2022, 1:15 p.m....https://doi.org/10.1177/33.8.3894499 Im K, Mareninov S, Diaz MFP, Yong WH (2018) An Introduction to... -
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog PostPublishedSept. 8, 2016, 2:30 p.m.... (e.g., transcyclooctene–tetrazine)– Nonnatural amino acids can be incorporated into a protein structure... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog PostPublishedAug. 9, 2016, 2:30 p.m....histone proteins K4 and K27 – locations of the amino acid lysine in H3 me1, me3, and ac – denoting ... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog PostPublishedOct. 22, 2015, 2:30 p.m....El-Bakkoury, M., Hamacher, T., Cappellaro, C., Vilarino, C., Fleischer, C., Ellerbrok, H., Kamuzinzi, ... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog PostPublishedOct. 14, 2015, 2:30 p.m....two nucleases, AsCpf1 and LbCpf1 (1307 and 1228 amino acids long respectively), both cleave in a staggered... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog PostPublishedOct. 6, 2015, 2:30 p.m.... sample that is transformed that week is then inoculated for storage on Thursday. The lab prepares bacterial... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog PostPublishedApril 23, 2014, 1:08 p.m....maximal biosafety available for the technology. The minor limitation in using the 3rd-generation system is... -
QC Sequencing Technologies at Addgene
TypeBlog PostPublishedJuly 15, 2025, 1:15 p.m....theoretical sequences submitted to Addgene have minor differences due to in silico variations and lack... -
Hot Plasmids: Fall 2024
TypeBlog PostPublishedNov. 21, 2024, 1:30 p.m....variation on their previous sensor STReTCh, the 11-amino acid BiT fragment of luciferase is embedded in the... -
Plasmids 101: Stringent Regulation of Replication
TypeBlog PostPublishedJune 20, 2024, 1:15 p.m....Solar, G., Giraldo, R., Ruiz-Echevarría, M. J., Espinosa, M., & Díaz-Orejas, R. (1998). Replication and... -
Antibodies 101: Reading a Flow Plot
TypeBlog PostPublishedFeb. 6, 2024, 3:13 p.m....Seroogy, C. (2017). Neonatal immune response to rhinovirus A16 has diminished dendritic cell function and... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog PostPublishedNov. 1, 2018, 12:35 p.m....PMID: 19678844. PubMed Central PMCID: PMC2906245. Danino, Tal, et al. "Programmable probiotics for detection... -
Neuronal labeling with Spaghetti Monster
TypeBlog PostPublishedAug. 14, 2018, 12:42 p.m....other advantage is that these tags are small (8-12 amino acids) and neither disturb POI folding nor its ability... -
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog PostPublishedMay 8, 2018, 1 p.m....circle in front of the sample name. If there was a minor error during processing, the window shows a yellow...