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We narrowed to 811 results for: lars

Showing: 201 - 220 of 811 results

  1. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...experiments ranging from transient gene expression in a particular cell type to CRISPR genome editing, optogenetics... also be used to ensure specificity; this is particularly helpful when wanting to target specific neuron...vector. Full-length ssAAV vector genome of both polarities are rescued from the vector plasmid and individually... conversion of the DNA goes through the inter-molecular annealing or second strand synthesis. In contrast...transcriptionally active double-stranded form through intra-molecular annealing. Figure and legend from Okada, 2013....Production System: Towards Gene Therapy For Duchenne Muscular Dystrophy. InTech. doi: 10.5772/53023 Veldwijk...method for the titration of AAV-2 vector stocks."Molecular Therapy 6.2 (2002): 272. PubMed PMID: 12349826...

  2. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...two are present; intramolecular events happen with greater frequency than intermolecular events, but any... kDa protein responsible for intra- and inter-molecular recombination at the loxP recognition sites. A...sequence between the loxP sites is excised as a circular piece of DNA (and is not maintained).  Translocation... is excised, and gene expression proceeds. One popular lox-stop-lox plasmid is from Tyler Jack's lab: ...purposes, including activating an oncogene only in a particular organ, or bypassing embryonic lethality. To ...site-directed recombination technology very conceptually similar to Cre-lox, with flippase (Flp) and the short flippase...

  3. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...pitfalls of accepting alternative facts. Because, similar to scientists, the legacy of each government and...scientific consensus and calling for billions of dollars in cuts to important research funding.  So when...sacrifice in April because devaluing science has alarming implications for our public policies—many of which...that the community of science supporters is much larger and stronger that I expected. Jennifer Stancill...Birmingham, AL, USA 33.5207°N 86.8025°W - Jonathan Aguilar Galileo wrote, “now you would not hide behind these... at the same time pass over in silence his very large one” (http://oll.libertyfund.org/titles/galilei-...deserve to know the truth about our world. Jonathan Aguilar (@AggleDetector on Twitter) is a graduate student...

  4. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...and uses the similar buffers as would be used for a mini prep, except it does it on a larger scale. Using...for standard molecular biology techniques Browse our video library Check out our molecular biology reference...able to do high throughput DNA preps and other molecular work if we need it to. This gives us the oppotunity...the Biomek FxP is it’s customizability. It has a large deck that can house many different types of devices... DNA purification We're developing ways to prep large quantities of DNA because some countries just can...

  5. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    Published
    Aug. 20, 2019, 12:31 p.m.
    ...to be large enough to target larger areas, typically a few hundred nl. Alternatively, for larger brain...activity, commonly via light-activated ion channels. Similarly, chemogenetic tools are designed channels that...also reduce expression levels of certain genes. A popular method uses Cre recombinase on transgenic flox ...lentiviral vectors are superior packaging vectors for larger genes - they can hold up to 8 kb, while AAV can...liquor of the cerebral ventricles (ICV, intracerebroventricular) to minimize tissue damage; however, the...

  6. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ... inherited by daughter cells during cellular reproduction, similar to the host’s chromosome, or they can...both Archaea and Eukaryota can also harbor these circular DNA passengers. Most natural plasmids encode beneficial...transfer can occur naturally through transformation (cellular uptake from the environment), transduction (viral... in a minute! Fun fact! Electroporation is one popular method scientists use to promote transformation...encoding these functions form the basis of many molecular biology tools! Figure 1: Common functions... Mother Nature’s toolbox for new and exciting molecular technologies!  This post was originally written...

  7. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...Biodesign Center for Molecular Design and Biomimetics and the School of Molecular Sciences at Arizona ...In response, the World Health Organization has declared a global health emergency and called for the fast-tracked...sensors within an extensive infrastructure of molecular and electronic companion technologies that has...1x106-fold lower than before), and our combined biomolecular tools can distinguish between global strains...detect the virus in a clinically relevant setting. Molecular diagnostics for the Zika virus predominantly look...Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components." Cell (2016). PubMed PMID: 27160350...

  8. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...coding the molecular biology of the worm into a computer model that recapitulates how molecular biology ...get involved a in variety of aspects from the molecular biology to learning how to grow and care for the...but we need to develop the manual skills at the regular microscope, and the scripting skills at the automated...locomotion. Our most recent models of C. elegans molecular biology are based on the standard worm genome....Center (CGC) at the University of Minnesota has declared our makerspace an educational institution meaning...Silicon Valley as a student and then instructor at Singularity University at NASA Ames, and an instructor at...

  9. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...250 kDa Similar in size 8% 20–250 kDa Similar in size 10% 10–100 kDa Similar in size ...Conversely, if your protein of interest is large, look for a larger pore size (0.45 µm).  Types of transfers...important part of your western is your antibodies, particularly your primary antibodies. We always recommend...When choosing your lysis buffer, consider the subcellular location of your protein. Proteins located in...to overnight to run, and is recommended for very large proteins. A semi-dry transfer is faster — 3–30 minutes...differing amounts of available epitopes for that particular antibody, this truly does provide a relative,...

  10. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...noise, due to the large amount of dsDNA in most starting materials, and is not particularly sensitive. The...Protocol: How to Design a primer  Molecular Biology Reference Molecular Cloning Techniques References Bell... copy number assays for years. qPCR works like regular PCR, just with signaling molecules added to the...be affected by DNA quality and primer design, particularly for amplicons under 100 bp (Ma, 2015.) A schematic...ll have to consider:  Resources needed Most molecular biology labs and departments will have qPCR machines...

  11. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...columns had similar DNA yields. Additionally, columns regenerated up to five times had similar DNA yields...Addgene.org Find protocols for molecular biology and cloning Read our Molecular Biology Reference Visit our...plasmid miniprep protocol from Addgene follows a similar workflow as a column-based plasmid extraction kit... This method provides DNA yield and quality similar to that obtained with commercial kits, but in less...cellulose-based method was faster, cheaper, and had a similar level of sensitivity. Figure 2: Steps to ...

  12. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...fluorophores conjugated to molecular species with high affinity for endogenous molecular targets such as paclitaxel...conjugate, and the complexity of the system. A particularly desirable property for small-molecule labeling...removing excess dye from the sample, which is particularly important for samples where washing is difficult...and fluorogenic ligands. New fluorophores As a corollary to these innovative labeling strategies, several... these fluorophores are often bulky and contain polar groups (Figure 2b), which can preclude their use... and structure at the single-molecule level." Molecular cell 58.4 (2015): 644-659. PubMed PMID: 26000849...

  13. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...Molecular cloning requires some method of screening colonies for the presence of an insert. Traditionally...the insert site. A positive clone will produce a larger size product then a negative clone without the ...determine their size. Make sure to use an appropriate molecular weight standard for reference and to add a loading...-) will not. Backbone-specific primers (2) give larger sized products for positive clones (+) compared..., colony PCR is a useful tool to have in your molecular biology tool kit. Consider giving it a try next...Tips and tricks from the bench Don't pick too large of a colony. Too many bacteria can inhibit your ...Resources on Addgene.org Read about Choosing a Molecular Cloning Technique Learn to Perform a Bacterial...

  14. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ... looking for some additional information or clarification on these vectors. Read on to find the answers...allow researchers to target transduction to a particular cell type or tissues; this is useful in gene ...therapy applications where a distinct subset of the cellular population needs to be targeted. Q6: Are transfer...self-inactivating, which is achieved by deleting a large portion of the 3'LTR in the transfer plasmid. The...not only the transgene but also that of adjacent cellular genes. Q7: Which genes are deleted or modified...through insertional mutagenesis. These risks are largely based upon the vector system used and the transgene...Library Resources on Addgene.org Addgene's most popular lentiviral plasmids Overview of Lentiviral Packaging...

  15. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type
    Blog Post
    Published
    July 23, 2019, 12:46 p.m.
    ...regions. PHP.eB, an enhanced version of PHP.B, shows similar transduction efficiency, but at a log lower dose...insertions and deletions (indels) most likely to have large phenotypic effects such as those that affected gene...pattern in the mouse brain.  Both labs then took similar routes to verify LY6A’s role as a receptor for ...transduce cells. The Wilson Lab generated results similar to the Deverman Lab when they looked at LY6A protein... B Transport across the Blood-Brain Barrier." Molecular Therapy 27.5 (2019): 912-921. PubMed PMID: 30819613... of AAV-PHP. B are limited to C57BL/6J mice." Molecular Therapy 26.3 (2018): 664-668. PubMed PMID: 29428298... across the blood-brain barrier: LY6A, a novel cellular receptor for AAV-PHP. B capsids." bioRxiv (2019...

  16. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...contributed by Kutubuddin Molla, a Fulbright Visiting Scholar at the Pennsylvania State University. Imagine you...Later the resultant U-G pair is either repaired by cellular mismatch repair machinery making an original C-G...’ to ‘I’ (inosine), which is treated as ‘G’ by cellular polymerases. Subsequently, an original genomic... is converted to a G-C pair (Figure 1). As the cellular inosine excision repair is not as active as uracil...Figure 2: Plant base editor platforms and their molecular components. Adenine base editors for plant.... Currently Kutubuddin is a Fulbright Visiting Scholar at the Pennsylvania State University, University...· T to G· C base editing in the rice genome." Molecular plant 11.4 (2018): 627-630. PubMed PMID: 29476916...

  17. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...codons altogether.  So what does this mean for a molecular biologist who wants to express genes from one ...might want to optimize codons for expression in a particular organism. Why is codon usage important? Translation...tRNAs to build a peptide. Image from Mariana Ruiz Villarreal. By the late 1980s, scientists even created... vivo (Slimko and Lester, 2003).  Addgene has a large selection of codon optimized genes, so check out...information encoded in the primary sequence of a particular gene which could affect translation initiation...optimization in human therapeutics. Trends in Molecular Medicine 20:604–613  https://doi.org/10.1016/j.molmed...Resources on Addgene.org Check out Addgene's Molecular Biology Reference Read about genetic code expansion...

  18. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...A similar genetic code is used by most organisms on Earth, but different organisms have different preferences...reasons include: Metabolic pressures - it takes cellular resources to produce tRNAs that recognize different...contains a gene that’s been codon optimized for a particular organism, this will sometimes (but not always... purification procedures. Even if you produce a large amount of protein in your expression host of choice...cotranslational folding." Nature structural & molecular biology20.2 (2013): 237. PubMed PMID: 23262490...bias as a means to fine-tune gene expression." Molecular cell 59.2 (2015): 149-161. PubMed PMID: 26186290...featured topic page Resources on Addgene.org Molecular biology reference pages Genetic code E. coli strains...

  19. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...publications Online tools like ResearchGate, GoogleScholar, Academia, ORCID, and MyScienceWork all help... especially published research, have grown in popularity over the last decade. Research by Scott Spicer...abstracts identified. The journal Cell had the largest average view count per video abstract at 2,947 ...being used to share science. TedTalks are very popular, though they focus mostly on highly innovative ...even use a computer.  Currently, two of the more popular biology videos are:     “Singing” Fish Rely ...Ryan at [email protected]   References 1. Larivière, Vincent, Yves Gingras, and Éric Archambault. ...and case study." Journal of Librarianship and Scholarly Communication 2.2 (2014): 2. Additional Resources...

  20. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    Published
    Dec. 20, 2017, 2:33 p.m.
    ...scientific or scholarly research contributions in the field Evidence of authorship of scholarly books or articles...should not be relied upon for legal advice in any particular circumstance or fact situation.  The information...agencies to propose reforms to the H-1B visa system. Large firms and firms that work on projects with an international... extraordinary ability category The EB-1A - is similar to national interest waiver criteria however the...showcases (not usually applicable to scientists). High Salary –not relevant to most postdocs. Commercial successes... articles (in scholarly journals with international circulation) in the field EB-1Bs historically have...documents that are not in English. Create a Google Scholar account Sanitize your LinkedIn, Facebook profile...
Showing: 201 - 220 of 811 results