...101 covers everything from plasmids parts and nomenclature to popular cloning techniques and troubleshooting... been fully updated with twelve new sections, including our popular "Dimers and Multimers" post. It's ...

...Addgene tiene una gran audiencia internacional, incluido trabajando con distribuidores de todo el mundo...  Addgene has a large international audience, including working with distributors across the world to ...

...find the original publication and ensure that it includes validation data relevant to your application —... makes the choice easy! Protein-based blockers include dry non-fat milk or serums like FBS, PBS, and BSA...typically spans 2–5 orders of magnitude, which includes a wide dynamic range for fluorescent proteins....

...engine May include validation data CiteAb Any Any Search engine May include validation data...

... there! Completed blog posts are also great to include on CVs and Resumes. An Expanded Network - Addgene...many different people. Recent Guest Writers Have Included: Wenning Qin, Molecular biologist with Biogen ...

...new edition of the eBook includes the details on advances in CRISPR including: Base editing Prime editing...

... that the new system could also be expanded to include even more loci. Figure 1: Schematic overview... validated and ready. “Currently, the kit only includes two versions of Cas9, nuclease and nickase, but...

...research in synthetic biology and bioengineering including a recent effort to construct a synthetic yeast...: Research projects underway in the Ellis lab including yeast and E. coli engineering, microbial cooperation...

...specific blockers, and the manufacturer will usually include recommended blocking conditions on the spec sheet...especially important for highly expressed proteins, including “housekeeping” or ubiquitously expressed proteins... to improve binding and/or reduce background, including trying longer incubations at both temperatures...

...other uses.   Cloning grade DNA Molecular cloning includes a set of techniques that are used to insert recombinant...this agent to increase transfection success.  In conclusion, it is important for you to establish in advance...

... Last year, we distributed 165,000 plasmids, including 27,000 CRISPR plasmids and 6,000 biosensors plasmids...antibodies. Everything in this collection, which includes both neuroscience and general-use antibodies, ...

... show resistance to stressful circumstances – including unpredictable loud noises and flashing lights ... treatments for a variety of brain disorders, including psychiatric and cognitive conditions as well as...

...you run a restriction digest on a gel you always include proper controls like uncut DNA and the proper ladder...amplification reaction (as opposed to using the all inclusive service some companies offer), you can purify ...

...engineering research. A variety of new tools came out including the single base editors, Casilio, CombiGEM, and...2A-Puro (PX459) V2.0: What's not in the name? Not included in this plasmid's name are some additional features...

...organization runs a wide variety of programs, including fall and spring lecture series, science cafes,... information about SITN: DayCon event website (includes archived content) Science in the News website ...

...that introduced the kit in December, GreenGate includes the six most frequently used elements in plant...works, just trash it.” Lohmann and his colleagues including Joachim Forner now expect and hope that other ...

... lentiviral packaging and expression vectors, including pMD2.G and psPAX2. Actually, these two plasmids...years after they were added to the repository, including beta-catenin reporter M50 Super 8x TOPFlash, scramble...

...on previously described pFA6a-link vectors and include a Kan, SpHIS5, or CaURA3 selection marker. Lee ... variety of genome modifications in S. Pombe, including full and partial gene deletion, overexpression...

...scientists in 88 countries around the world. That included 2008 pooled libraries, 290 antibodies, 18,359 ...it more accessible. Other accessibility updates include a printer-friendly design and alt text on all images...

...Statistics (OES), but international Twitter users were included in the dataset; 2) users not part of a scientific...scientists who follow each other, or are friends, are included in this cluster analysis. The closer two dots ...