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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 629 results for: ncl

Showing: 201 - 220 of 629 results

  1. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ... in Table 2 is a standard and should always be included—the live/dead stain. This stain is in fact not...www.bdbiosciences.com/en-us/resources/bd-spectrum-viewer.   Conclusion You made it! In this post, you learned about ...Sharpe at Harvard Medical School. His projects include investigating negative regulators of T cells and...

  2. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...impediments. FRET has been used in many applications, including drug discovery by big pharma (i.e. HTRF and LanthaScreen...dynamic range of your assay. These experiments include producing a positive control construct where the... Women’s Hospital in Boston, MA. His interests include developing novel fluorescent tools for labeling...

  3. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ....  Of course, proper controls are required and include, for example, confirmation of opsin expression ... brief, some standard opsins (see table above) include Channelrhodopsin2 (Chr2), a blue-light sensitive... at the Rockefeller University.  His interests include the role of learning and memory in opioid addiction...

  4. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...Scorpions Low Probe is on a hairpin structure included in the 5’ primer Yes Yes. Agarose gel  ...Low Reporter and quencher on hairpin structure included in primer Yes Yes Agarose gel Table...well-to-well normalization of signal. They are often included in commercial sets and most machines have a protocol...

  5. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...few references for the double nickase system, including one from the Zhang group. Q2: Should I add the...off-target effects depends on a number of factors, including: how closely homologous the off-target sites are.... Thank you, Le! This blog post was updated to include all FAQs from Le Cong on June 24, 2025 by Alyssa...

  6. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    Published
    Sept. 5, 2023, 1:15 p.m.
    ...101 covers everything from plasmids parts and nomenclature to popular cloning techniques and troubleshooting... been fully updated with twelve new sections, including our popular "Dimers and Multimers" post. It's ...

  7. CRISPR 101 eBook...en español

    Type
    Blog Post
    Published
    Jan. 18, 2024, 2:15 p.m.
    ...Addgene tiene una gran audiencia internacional, incluido trabajando con distribuidores de todo el mundo...  Addgene has a large international audience, including working with distributors across the world to ...

  8. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...find the original publication and ensure that it includes validation data relevant to your application —... makes the choice easy! Protein-based blockers include dry non-fat milk or serums like FBS, PBS, and BSA...typically spans 2–5 orders of magnitude, which includes a wide dynamic range for fluorescent proteins....

  10. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ... there! Completed blog posts are also great to include on CVs and Resumes. An Expanded Network - Addgene...many different people. Recent Guest Writers Have Included: Wenning Qin, Molecular biologist with Biogen ...

  11. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...new edition of the eBook includes the details on advances in CRISPR including: Base editing Prime editing...

  14. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...specific blockers, and the manufacturer will usually include recommended blocking conditions on the spec sheet...especially important for highly expressed proteins, including “housekeeping” or ubiquitously expressed proteins... to improve binding and/or reduce background, including trying longer incubations at both temperatures...

  15. Plasmid Preps: Different Purity, Different Quantities, Different Uses

    Type
    Blog Post
    Published
    June 16, 2020, 1:25 p.m.
    ...other uses.   Cloning grade DNA Molecular cloning includes a set of techniques that are used to insert recombinant...this agent to increase transfection success.  In conclusion, it is important for you to establish in advance...

  16. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    Published
    Feb. 23, 2023, 2:15 p.m.
    ... Last year, we distributed 165,000 plasmids, including 27,000 CRISPR plasmids and 6,000 biosensors plasmids...antibodies. Everything in this collection, which includes both neuroscience and general-use antibodies, ...

  17. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    Published
    Nov. 25, 2014, 4:02 p.m.
    ... show resistance to stressful circumstances – including unpredictable loud noises and flashing lights ... treatments for a variety of brain disorders, including psychiatric and cognitive conditions as well as...

  18. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

    Type
    Blog Post
    Published
    June 26, 2014, 2:23 p.m.
    ...you run a restriction digest on a gel you always include proper controls like uncut DNA and the proper ladder...amplification reaction (as opposed to using the all inclusive service some companies offer), you can purify ...

  19. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ...engineering research. A variety of new tools came out including the single base editors, Casilio, CombiGEM, and...2A-Puro (PX459) V2.0: What's not in the name? Not included in this plasmid's name are some additional features...

  20. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    Published
    June 17, 2015, 9:04 p.m.
    ...organization runs a wide variety of programs, including fall and spring lecture series, science cafes,... information about SITN: DayCon event website (includes archived content) Science in the News website ...
Showing: 201 - 220 of 629 results