We narrowed to 397 results for: organization Addgene

... resources on addgene.org  Addgene's CRISPR Guide Addgene's CRISPR 101 eBook  Addgene's CRISPR Cheat Sheet...distinguishing the foreign sequences and keeping the array organized. Target strand: The strand of foreign DNA that...looser PAM requirements, which are available from Addgene!   RNA crRNA: “CRISPR RNAs” are transcribed from...CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer to the programmable guide sequence...Conclusion Ready for some more details? Check out the Addgene CRISPR guide or our CRISPR 101 ebook. We also ... Sheet  Resources on the Addgene blog A Needle in a Base-Stack: Cas9 Structural Biology...

...power of community in 2024 In 2024, your trust in Addgene helped unlock unprecedented scientific collaboration.... Even more remarkably, 1,160 labs from 522 organizations in 41 countries enriched our repository with...online and in-person, you helped us understand how Addgene can better serve the global scientific community...about our latest developments? Subscribe to the Addgene newsletter. References Ayoubi, R., Ryan, J., Gonzalez...

...manipulation Resources on Addgene.org Browse all viral vectors available at Addgene Visit our viral vectors...tools for optogenetics and chemogenetics on the Addgene website. Optogenetics also requires that light ...brain. One way to address this is by working with organisms with light accessible brains, such as zebrafish...bs.mie.2018.01.022 Additional resources on the Addgene blog Read more about optogenetics New to viral...

... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR.... Caulobacter crescentus is a popular model organism for studying the bacterial cell cycle, asymmetric...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...more information. Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by...

...for a review, see Simpson et al., 2015). Visit Addgene's MIcrobiology Pages The problem: Host binding proteins...membrane on an agar plate selective for your target organism and incubate overnight according to appropriate...on hand that will specifically bind your target organism, it can be helpful to spot this protein directly...PubMed Central PMCID: PMC2258639. Resources on the Addgene Blog Learn How CRISPR Can Be Combined with Phage...CRISPR For Genome-Wide Screens Resources on Addgene.org Visit Our Microbiology Pages Use the FX Cloning...

...the supplement of this paper), help each person organize his or her thoughts to facilitate honest conversation...for their time in the lab and their next steps. Addgene would love to hear about the steps taken in your...

... OR enzyme activity. Small molecule biosensors Addgene has many sensors that measure the concentration...interaction, thereby changing the FP’s structural organization and activating the fluorescent signal. Blue ...fluorescent molecule   Additional Resources on the Addgene Blog Check out the Rosella Autophagy Sensor Read...Addgenie Mary Gearing contributed to the content of this article. Biosensors (‘biological sensors’) are...extracellular molecule? By fusing a biosensor to an organelle-specific targeting signal or a transmembrane domain...CRISPRainbow for Genome Visualization Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids...

...reagent type, target species, target name, host organism, isotype, clone details, vendor, method, tissue.../10.1073/pnas.2018488117 More resources on the Addgene blog Antibodies 101: Multiplex Immunofluorescence...Immunofluorescence Negative Can Be Positive: Open AAV Data with Addgene Antibodies 101: Choosing the Right Isotype...

...perfect job.   Resources at Addgene Read Additional Career Posts on the Addgene Blog Browse Our Careers ...Luckily, I have worked for a variety of different organizations, so I tend to provide them with an overview ...practices, but can sometimes go unnoticed at large organizations. Another downside to large commercial companies...applied anyway. I later found out the job was with Addgene. I had used plasmids from this company before and...also get the chance to contribute content to the Addgene’s Website and travel to meet scientists, making ...develop my own projects, but working remotely (Addgene’s main offices are in Cambridge MA, while I’m based...Careers Page to See What Positions Are Open at Addgene Learn How to Choose a Good Mentor for Your Professional...

...deposited these plasmids at Addgene. While deposits from for profit organizations are less common, they can...This year, Addgene welcomes three new members to the Board of Directors: Khandan Baradaran, Frank Stegmeier...assay technologies makes her a perfect fit for Addgene. She was Head of Quality at Dimension Therapeutics... the research funded,” she says. Baradaran met Addgene Executive Director Joanne Kamens several years ...through this connection that she learned more about Addgene. “The mission to share critical reagents such as...Scientific Officer at KSQ Therapeutics and is an Addgene depositor. KSQ uses a CRISPR-Cas9 platform to systematically...finds the value in the wealth of resources on Addgene’s website and blog important. “The experience from...

...Doudna, Feng Zhang, and Keith Joung. All Addgene depositors! Addgene was mentioned specifically in Dr. Zhang's...harnessed. Sharing CRISPR, synbio, & more via Addgene Addgene's addition to the poster session was a first...happens): Science is hard. Chris Voigt, another Addgene depositor, discussed some of the many challenges...Simon Singh's "The Code Book" for this month's Addgene Book Club. I read most of the book on my way to...from those who expressed gratitude for the role Addgene plays in plasmid sharing to those who were anxious...this meeting. And of course, I want to thank the organizers of this meeting who have given the attendees ...increasingly important field.  Interested in Addgene's Keystone Poster?  We'll be sharing the poster...

... rigorous research plans. However, amidst the organized chaos of lab research and PhD studies, there lies...and execution plans.    More resources on the Addgene blog Experimenting with New Careers While in Grad...

... If you start poking around on Addgene’s Fluorescent Protein Guide to In Vivo Imaging, you’ll pretty ...peering right through living animals to see their organs – a liver or brain, say, or maybe a tumor. They...for optical labeling and tracking of proteins, organelles, and living cells in a spatiotemporal manner,...  Kendall Morgan...

... biology tools.  Addgene Data Hub: A data repository for validation data on Addgene/CLOVER viral vectors...you keep your virtual molecular biology toolbox organized, today’s post features a list of free online molecular.../ protein database search and analysis tools Addgene's analyze sequence tool: Annotates features, BLASTs...restriction enzyme cut sites Open reading frame finder  Addgene vector database: Find sequences for plasmids assembled...available sources, as well as those deposited with Addgene. NCBI BLAST: Compares nucleotide or protein sequences...tool, set up an IDT account. Figure 2: Addgene's Analyze Sequence tool.   DNA sequence manipulation... Rachel Leeson.  Additional resources on the Addgene blog: Learn how the CRISPR software matchmaker...

...guest writers. Your contributions make Addgene and the Addgene Blog useful resources for the biological...It’s nearing the end of #DepositorWeek at Addgene and we’d like to show our gratitude on the blog by ...highlighting some of the great posts written by Addgene depositors. Below you’ll find brief summaries of...depositors. Interested in writing your own post for the Addgene Blog? Fill out our guest blogger form to get started... section of DNA into the genome of your target organism. In this post, Chris Richardson, a postdoctoral.... Note: Casilio is no longer available through Addgene.  Thanks again to all of our fantastic depositors... addgene...

... What is an Antibody? Resources on Addgene.org: Browse Addgene’s Antibody Plasmid Collection Find antibodies...cells or tissue slices mounted to a slide, or whole organs. Because the tissue architecture is preserved, ...from the bloodstream. Then, via a blood draw or organ harvest, the labeled cells can be analyzed using...different research applications they can be used for. Addgene’s Antibody Plasmid Collection includes plasmids ...10.3389/fcimb.2013.00072 Additional resources on the Addgene blog: Read about another reagent sharing platform...

...When I started writing for the Addgene blog, I was focused on writing about new scientific techniques...first program I used to create graphics for the Addgene blog. However, it has a limited number of drawing...Medical Art This site has 3,000 free medical images organized into four main categories: anatomy and the human...comments section!  Additional resources on the Addgene blog Read our Early Career Researcher Toolbox ...

... about the role these factors played within an organism.  So how did plasmids get their name? In 1952...diversely applied to many different experiments. Addgene was founded in order to store, QC, curate, and ...cloning and gene expression. Just released: Addgene's Plasmids 101 (2nd Ed.) eBook And now for some ..... You can now download the newest version of Addgene's Plasmids 101 eBook! In keeping with our goal of...

...skills guide Resources on Addgene.org Learn more about Careers at Addgene Watch out career videos   ...skilled at presenting and answering questions. Organizing a thoughtful engaging talk takes significant ...successful writers about their strategies for organizing and self-critiquing their own writings. 8. Learn...outdoor photographer.  Additional Resources on the Addgene Blog Find Tips for getting a faculty position ...

... will make specific, controlled changes to an organism’s genome (genome engineering) Produce synthetic...that can be used in research or for therapeutics Addgene has compiled various educational resources to facilitate... facilitate plasmid use in the lab. Addgene’s Molecular Biology Reference includes information about molecular...