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We narrowed to 388 results for: plasmid dna

Showing: 201 - 220 of 388 results

  1. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...deposits with more than 10 plasmids is to use our deposit spreadsheet?  Our plasmid deposit process can be... Send plasmid information to Addgene Material Transfer Agreement (MTA) approval  Send plasmids to Addgene...instructions on how to send liquid DNA or bacterial streaks of your plasmids back to us. Our scientists will...spreadsheet and copy and paste your plasmid data directly into our file, one plasmid per row, as outlined by the... Data Field Description Plasmid Name Use a descriptive plasmid name and match the name as it ...application of the plasmid and will help a scientist outside your field understand if this plasmid will work for...possible, we propagate plasmids in the standard cloning strain DH5α. For plasmids with highly repetitive...

  2. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    Published
    Dec. 10, 2019, 1:58 p.m.
    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...need. If you'd ever like to write about a recent plasmid deposit please sign up here.        A retroviral...transducing a only a single gRNA per cell. The viral DNA containing the gRNA then integrates into the genome...identified by next-generation sequencing of the genomic DNA. However, some cell types are poorly transduced by...treatment of cardiovascular disease. Find the LipoGlo plasmids at Addgene! Lawler et al, QJM: An International...promoter systems. Find these CRISPR activation plasmids at Addgene. Liu et al., Nature Communications,...

  3. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...connectors, cDNAs, and more to find the perfect combination. The EcoFlex Kit has a wide array of DNA parts ...Modular cloning is a popular DNA assembly tool used to build single- and multi-gene constructs for a ...background MoClo is a clever way to assemble several DNA parts in a specific orientation using Type IIS restriction...between CRISPR components expressed from different plasmid backbones. They noted that backbone differences... as the order, or distance in the placement of plasmid elements can impact expression levels and consequently...system was used to assess how different lentiviral plasmid backbone elements impact viral titer and to compare...plants, and animals (Chiasson et al., 2019). Many plasmid elements such as promoters or fluorophores are ...

  4. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type
    Blog Post
    Published
    April 23, 2018, 2 p.m.
    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need... our hot plasmid articles from April 2018. If you'd ever like to write about a recent plasmid deposit ...sign up here. No time to read? Listen to our Hot Plasmids Podcast! TRIM-Away: Targeted endogenous protein...segment CRISPR and RNAi have helped researchers alter DNA sequence and RNA expression - but what if you just...your protein knockdown experiments! The TRIM-Away plasmids can be found here. Clift et al. Cell. 2017. PubMed...Endosomal pH Sensors podcast segment The first Addgene plasmid deposit from Professor Stefan Hell, who won the...

  5. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    Published
    Feb. 13, 2014, 1 p.m.
    ...letter of a plasmid name and simply denotes that the object is a ‘plasmid’. “p” is for plasmid. pXXXXX-XXXX...insert in your plasmid name. This is often a 3-6 letter representation of the gene (or DNA sequence). pBACKBONE-hGene...information about plasmid features and tips you can use in the lab, check out more posts in our Plasmids 101 series...There are no universal rules for naming plasmids but here are some good guidelines to follow in order...people can quickly and easily identify what your plasmid contains and other important information. Tip:...Backbone name Include the empty backbone name in your plasmid name. This simple piece of information can often...important details. Once you know the backbone a plasmid is based on, you can usually derive: a) the bacterial...

  6. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...can use PCR to make small sequence changes to plasmid DNA. This an oldie but a goodie, and in my personal...that describe methods to make single base edits in DNA. The first, contributed by guest blogger Kristian...experience, is a quick way to generate many mutated plasmid constructs. The second post was contributed by ...

  7. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head...AAV-deliverable ABE.  Find the plasmid here Jesse Zalatan’s lab deposited plasmids for a chemically inducible...need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's what you'll...mGold, a new yellow fluorescent protein New CRISPR plasmids New items from the viral service A fast blue-light...recognize, cut, and alter specific target sequences in DNA resulting in a variety of outcomes including excision...

  8. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...- that is, since you’re looking at the amount of DNA in your sample relative to your normal control - ...dye that works by binding to all double-stranded DNA, while Taqman uses an intercalating dye, with a fluorescent...The fluorescent signal can be affected by AT-rich DNA and the length of the amplicon and it has a higher...In both applications, results can be affected by DNA quality and primer design, particularly for amplicons...statistics), and the readout gives an absolute value of DNA concentration. By comparing the number of positive... number using SYBR Green: confounding by AT-rich DNA and by variation in amplicon length. Am J Trop Med... RJ, Paterson ES, Smith ML. The copy-number of plasmids and other genetic elements can be determined by...

  9. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...Unpublished or pre-publication plasmids": Unpublished plasmids or plasmids that will not be published are...and entering a plasmid name,plasmid type, and plasmid description/experimental purpose (right). On the...with your plasmids, and adding all the plasmids at once increases the likelihood that the plasmids are given...liquid DNA with all of the plasmids in the library in a single tube. The number of unique plasmids in a ...easy for you to share plasmids. To achieve this goal, we will archive any plasmids you've deposited with...Spreadsheet". Start your plasmid deposit There are two options to begin your plasmid deposit. Figure 2: Choose... Choose to submit plasmid(s) online or by using a spreadsheet. The plasmid information can be entered ...

  10. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type
    Blog Post
    Published
    May 4, 2021, 1:15 p.m.
    ...interest has been cloned into an expression plasmid, the plasmid can be introduced into host cells, such ...that the plasmids and sequences for antibodies are not shared. For instance, through open plasmid sharing...institutions have proposed a shift to recombinant DNA-based antibody technologies (Bradbury & Plückthun...Recombinant monoclonal antibodies are encoded in plasmids and produce a single antibody recognizing one ...synthetic genes that are typically expressed from a plasmid or from an integrated sequence in a stable cell...heavy chain genes of the antibody are encoded in a plasmid. (Right) An assembled antibody protein binds an...antibody can be converted into recombinant form through DNA sequencing of the line and subsequent cloning of ...

  11. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...They generated plasmids tagged with EB3 to mark the growing tips of microtubuli and plasmids tagged with ...and dCas9-VPR targeting plasmids can be found at Addgene. Check out these plasmids for piggyBac gRNA delivery...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head... Find the plasmids at Addgene The MoClo CRISPR/Cas Toolkit for plants includes 95 plasmids consisting...need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's what you'll...

  12. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ....1111704108. Epub 2011 Aug 9. PubMed. Find the Plasmids @Addgene: Plasmids for KD, R, B2, and B3 recombinases from...frequency of 1.2 per megabase in mammals), leading to DNA damage and developmental aberrations. In multiple...10.1038/ni0707-665. PubMed. Growth inhibition and DNA damage induced by Cre recombinase in mammalian cells...

  13. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...ddPCR) Historically, a quantitative DNA hybridization method (DNA dot blotting) had been used to titrate... as opposed to purified DNA. This may be mitigated by using purified viral DNA as the template instead...the category: ELISA...qPCR...Digital droplet PCR...DNA dot blot...Transduction assay...SDS-PAGE...Electron...concentration of viral genomes (by qPCR or other DNA quantification methods - see below), since each viral...concentration. We regularly make and validate fresh plasmid standards. We validate our absolute quantification... digital PCR system for absolute quantitation of DNA copy number." Analytical chemistry 83.22 (2011): ...

  14. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...skin, blood and semen samples. Ma et al. designed plasmids containing Cas9 and two different gRNAs, and electroporated...oocytes, thus allowing repair to take place before DNA replication. Indeed, they observed that 41/42 embryos...editing.’ While repair using endogenous chromosomal DNA is appealing for heterozygous mutations, it will ... homozygous mutations. Moreover, introducing new DNA seems impossible with current tools, thus quieting...CRISPR Resources on Addgene.org Browse All CRISPR Plasmids Check Out Our CRISPR Guide Pages ...

  15. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ... its reported cleavage site on the complementary DNA strand. And this is why we love working with researchers... have in order to scan along the double-stranded DNA, recognize a PAM sequence, allow the complementary...base pairing between the guide RNA and the target DNA strand, and then activate two different endonuclease...This structure captures the displaced non-target DNA strand and shows the HNH nuclease domain swung around...juxtaposition to its cleavage site on the target DNA strand. In addition, this model also displays the...Guide Page CRISPR/Cas9 History Browse All CRISPR Plasmids and Resources ... technology to 4th graders, check out Jennifer Doudna’s new book A Crack in Creation. Making models more...

  16. Plasmid Grant to Aid Cervical Cancer Screening in Ghana

    Type
    Blog Post
    Published
    Nov. 8, 2016, 3:30 p.m.
    ... Addgene/Seeding Labs plasmid grant and plans to use the grant to order plasmids she can use as positive...similar grants back in June, Gladys will be using plasmids in creative ways that we wouldn’t necessarily ...commonly used in Ghana, there is little screening for DNA markers like the presence of high risk (HR) Human...HR HPV types, predominantly HPV 16 and 18.” HPV plasmids as positive controls To start her work, Gladys...cytological tests currently in use. Gladys will use plasmids containing a variety of HPV genes as positive ...incredibly happy to hear that Gladys will be using plasmids in a way that can potentially have a direct impact...awardees We will be opening the Addgene/Seeding Labs Plasmid Grant to a third round of applicants at the start...

  17. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    Published
    Aug. 13, 2019, 12:58 p.m.
    ...Read our plasmids 101 blog series Resources on Addgene.org Browse our zebrafish CRISPR plasmid collection...Creating transgenic organisms by introducing foreign DNA into the genome is useful for a variety of applications...transposon called Tol2 to randomly integrate foreign DNA into the genome. Non-random integration into specific...facile CRISPR/Cas9-mediated insertion of foreign DNA. Despite these caveats, several features of zebrafish—including...collection Read our molecular biology reference Find plasmids for your research ...

  18. 5 Science Rap Videos & Their Creator: Tom McFadden

    Type
    Blog Post
    Published
    Feb. 9, 2017, 3:30 p.m.
    ...the bay area to create a plasmid rap video for us (If you’re new to plasmids, we highly recommend checking...about colony collapse disorder.     5. Plasmid Rap Plasmid rap video made with support from Addgene....incredibly important role in solving the structure of DNA. 2. The Immortal Rap of Henrietta Lacks Students...Podcasts Find Great Educational Content in our Plasmids 101 Series Additional Resources on Addgene.org...

  19. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...Isotype switching is caused by a specialized type of DNA recombination. Heavy chain constant genes are clustered... expressing a different isotype, the intervening DNA sequence is deleted, which places the variable region...    Figure 2: Isotype switching requires DNA recombination of the antibody gene locus. B cells...in the heavy-chain constant domain gene cluster. DNA recombination can delete intervening heavy-chain ...Resources on Addgene.org Browse Addgene’s Antibodies plasmid collection ...

  20. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...Feng Zhang’s lab at MIT, the use of customizable DNA-binding domains based on transcription-activator-... (blue) light switch. Zhang’s team devised the plasmids now available in Addgene’s repository for use ...biological processes and disease states. While LITE plasmids have clear application to anyone with an interest...
Showing: 201 - 220 of 388 results