...stage-specific promoter and design a UAS reporter construct, the reporter will only be expressed where and/or...UAS to examine the expression of reporter genes, or to combine reporter expression from one promoter with...the Q system from a gene cluster in the fungus Neurospora crassa. The QUAS promoter is less leaky than ... transcription from the UAS promoter. This is important because it allows them to be used together for...

...useful for genetic engineering approaches. Most importantly for this technology, however, is that the transposase...should be tested in the cell line of interest. Importantly, by varying these ratios, you can either maximize...obstacle, a positive selection cassette can be incorporated, and used during the differentiation process...homologous recombination, which results in the incorporation of your specific edit into the host genome. ...transposon sequences.  The use of PBx is particularly important to avoid any possible random re-integration from...lines, most likely due to DNA toxicity. More importantly, unlike Cre recombinase, the PBx is highly specific...

...scientists with an MSc or higher apply. It was important to weigh the cost to benefit of studying a Masters.... I felt then, and still feel now, that it is important to take advantage of the fact that science is ...manufacturing practice (GMP) and good lab practice (GLP) (important buzzwords in industry) as well as skills in industry... Queensland in Australia, this was a perfect opportunity to work in a genetics lab. As a UK citizen, I...and to discover for myself what potential job opportunities are actually like day-to-day. It is all too ...permanent position in academia. It is therefore important for young scientists and PhD students to also ...

...means that scientists-in-training rarely get the opportunity to learn about the myriad of diverse, nonacademic...questions on the internet. To make the most of the opportunity, use a written list and take notes if that is...time to ask personal questions about salary, opportunities for advancement, challenges and disappointments...respond to you). You will uncover interesting opportunities by meeting with people working in diverse and...That way you are always setting up your next opportunity to learn. Additional Reading More "Career Advice...

... is resistance to change, public support is weak, government support is “feckless”.  So what can be done...from Harvard Medical School and a long list of supporters from all of the research centers in Boston.  ...for PIs to include in their annual NIH progress reports information on the Individual Development Plans...will come up with to push for change in their opportunities.  Scientists must do science to be happy so ...

...background.” Just last month in Scientific Reports, Verkhusha’s team reported another advance in the application.... Transparency window That’s exactly what they reported doing in a paper that appeared in Nature Biotechnology...they absorb and emit light in the near-infrared portion of the electromagnetic spectrum – the spectral ...proteins, organelles, and living cells in a spatiotemporal manner, which is not possible with conventional...

...piggyBac plasmids    Tol2  Tol2 was the first reported active DNA transposon in vertebrates. It was discovered...mutations. In these screens, the transposons encode reporter genes, mutagenic cassettes, or barcodes. When ...2021). Target site preference and host range are important to consider when selecting a transposon for a ...pronuclei of fertilized eggs, which leads to random incorporation of that sequence into the zygote’s genome, a...unpredictable. Transposons, however, efficiently incorporate into the zygote’s genome after injection into...Sandoval-Villegas N, Nurieva W, Amberger M, Ivics Z (2021) Contemporary Transposon Tools: A Review and Guide through...

...orchestrate the formation of high-functioning-but-temporary teams that have a specific purpose and finite ...year, or a few months, or even a few weeks. The important thing to remember is that every team should have...when I do come across one. Also, remember, one important feature that all projects have in common - and... What does a good team need? Besides that all-important sense of purpose, there are many more things necessary... function is certainly necessary, but equally important is making sure all team members understand what...their responsibilities are. That’s a subtle but important distinction. If I invite someone to be on a project...

... of single-use items, plastic is a substantial portion of lab waste. Despite the fact that researchers... NEB actually started this program in 1976! Millipore Sigma takes back expanded polystyrene boxes for...environment? Well, you could: Collect the NEB and/or Millipore Sigma boxes from your lab or department and send...challenge you to take to action! These days, it’s important for individuals, companies, and universities to...to work on this issue. Be a champion for this important cause! To get more lab sustainability tips, check...

...consider as you make your choice. The first and most important thing you need to know is your expression system...have to make sure that your plasmid has been incorporated into the host organism, usually achieved with...basal expression level, induce expression at an opportune time or go all out with constitutive high level...Some of the more popular studies involve using a reporter vector to tag YGOI with lacZ and/or GFP (e.g. ...animals, gene targeting and genome modifications to reporter vectors (e.g. pBV-Luc) and gene silencing (e.g...

...’s ~4.4 kb or smaller. Some examples include a reporter like GFP, some smaller CRISPR-Cas proteins such...the Cre recombinase, are used to spatially and temporally control transgene expression. Lox sites are directional...sites lets researchers control the spatial and temporal activation of a transgene. Figure 2: FLEX...structure when transcribed which aids in nuclear export of mRNA and helps increase the expression of your...polyadenylation or polyA signal aids in the nuclear export of RNA and RNA translation, and promotes RNA transcript...

...and have always been, a community resource that supports both toolmakers and tool users. We encourage you...Educational Resources As part of our commitment to supporting your research, Addgene is creating an array of...Through our incredible community, including your support, we have enabled scientists to easily share plasmids...Studies Hybridoma Bank, a nonprofit that has been supporting hybridoma sharing within the community for decades...Immunofluorescence Addgene’s Antibody Guide The project is supported by a NIH BRAIN Initiative grant (U24NS119916 )...

...scientists who have knowledge of their product portfolio. After all, you have been their target market ...throughout their career?”, "Have they sought out opportunities to expand their skill sets?", “Have they succeeded... interviews Studying for interviews is really important. For my Postdoc position, I tried to read at least... current sales position interview was just as important. The problem was I knew I had a knowledge gap....teaches you about selling products which is an important part of the business and allows you to work with...

...sub-cellular localization signals, plant markers, and reporters (Engler et al., 2014). This kit is a great starter...upon (its ‘basics’ MoClo plant kit buddy). It is important to understand your experimental needs before choosing...into a multi-TU targeting construct (Cas9, sgRNA, reporter, etc.). This allows for the multiplexing of sgRNAs...basic biology of and gene regulation in the opportunistic pathogen Acinetobacter baumannii (Brychcy et...used successfully for protein localization and transport studies in HEK cells, recombinant protein purification...Multi-Kingdom Golden Gate Cloning Platform.” Scientific Reports, vol. 9, no. 1, Springer Science and Business Media...

...be it an organic dye or FP) is one of the most important steps in the design of an imaging experiment.  ...Goedhart on the Addgene blog – outlining various important considerations when choosing a FP, but much of...valid email address).  One may also simply attach important references that serve to further characterize ...proteins.  For computer-savvy users looking to incorporate FPbase data in their own projects and applications... and elaboration.  Current projects include incorporation of biosensors, protein evolution lineages, 2...

...this year, I jumped at the chance. Here was an opportunity to see Shinya Yamanaka celebrate the tenth anniversary...different fields. This meeting has given me an opportunity to dive a little deeper into the world of stem... most scientists recognize and appreciate the importance of ethics, the topic can often take the back ...conversations but they frequently seem to be of secondary importance (or tertiary, or non-existant). On the very first... the stem cell community for addressing these important questions that the scientific method cannot address...

...ready to support your team and take responsibility if things go wrong. Here are some important criteria...distributed appropriately. If team members have opportunities to develop and stretch then they will be happier...want to know about right away? Provide adequate support. The manager must be available to answer questions...shared document. Update frequently with progress reports, meeting minutes and plan changes. Written documentation...

...aspects of my work but also found fulfilment in supporting my team’s development and career growth. This...check-ins, which helped them stay on track and feel supported. Another team member was highly self-motivated... challenges. Experimenting with leadership opportunities before fully stepping into a managerial role...as they encouraged me to explore small-scale opportunities where I could make mistakes and learn from them... served as a great "baby project," offering a supportive environment to build my management skills. Finally...

...function in terms of its regions. But equally important is the way the brain connects across these regions...is connected to itself across these regions is important for understanding how the brain functions. The...associated virus (AAV) shows relatively low retrograde transport efficiency in most serotypes. Some viruses (e....e.g., rabies) naturally exhibit retrograde transport and can therefore perform retrograde tracing, but...requires high titers for efficient retrograde transport. For recombinase-dependent vectors, this can cause...

...expression in the liver after systemic delivery.  Importantly, this is true in both mice and nonhuman primates... a cleavage free manner. The dCas9-SSAP system reports an efficiency of up to 20%, comparable to commonly... For a one kilobase insertion, the researchers reported an editing error rate of less than 0.3%, both ...using mStrawberry, localization peptides, and reporters that drive the expression of the fluorescent protein...response element (TRE) or kinase translocation reporters (KTRs). Find visual barcode plasmids here! Kaufman...