We narrowed to 465 results for: val

...details. Not only will these mStayGold variants be valuable tools for your next experiments, they will also...expression than AAV9. This new capsid will be a valuable tool for investigating NHP brains. Figure...

... can result in the retention of introns or the removal of exons. Disrupting promoter or transcription ...) deletion. (B) Mutations of splice sites. (C) Removal of an exon or a large genomic fragment. (D) Knock-in...

...Additionally, controls available in the system are validated and were chosen because of their broad applicability...process down into four easy steps, provides the real value for the user. Let's walk through designing a CRISPR...

...antibody options for your experiment (all well validated for your application, of course!) When the stars...these. After all, there’s no sense in spending valuable research dollars on a new secondary antibody if...

... exactly what antibody they are using and any validation experiments performed will be useful in perpetuity...518027a Bradbury AM, Plückthun A (2015) Antibodies: validate recombinants once. Nature 520:295–295 . https:...

...leadership training began with the NPA where I gained invaluable experience working on and later chairing the ...and just recently stepped down. I have gained invaluable experience and even after 5 years, was still ...

...remain unaware that EGFP/GFP is prone to forming noncovalent dimers. This property of EGFP can lead to significant...EGFP/GFP has a real nontrivial propensity to noncovalently dimerize. That means that your POI fused to ...

... on to the next thing. If I made a mistake, I evaluated what lessons I had learned – call it “troubleshooting...Additional Resources I asked some colleagues to share valuable life lessons they have learned during their scientific...

...spectrum of fluorescent proteins (FPs) has been an invaluable resource for studying the organization and function...detected by labor-intensive processes like PCR or by evaluating visual phenotypes. Improving C. elegans fluorescent...

...contaminant is Addgene plasmid #44362. Method validation, limitations, and future plans How did AAV-68544...purposely mixed another AAV vector with AAV-68544 to validate our method. We then extracted the DNA and processed...

...and of modulating gene expression makes it an invaluable tool in the interrogation of gene function. However...transcriptional and epigenetic regulation have been validated with these proteins. Furthermore, among those ...

...Forward genetics screens are a valuable part of the molecular biology toolbox to identify new target ...working with mice and slightly less red tape for approval processes. Additionally, while both AAV and lentivirus...

...Each hit from the screen should be independently validated to ensure that the desired modification produces...place. With the proper experimental design and validation practices, CRISPR libraries can help you learn...

...making all the tools for plant research here and validating them, it made the most sense to us to enable ...women in science, and many other topics. What’s the value of engaging about science in that way for you? Patron...

...receiving parts." "We have found an amazing and valued partner in Addgene," Endy added. "The phrase we...professional public-benefit partner who shares our core values." Addgene spoke with Endy more about the BIOFAB...

...phosphopantetheinyltransferase) – These enzymes catalyze the covalent attachment of a fluorophore ligand to a peptide...and manipulating proteins in live cells through covalent labeling." Nature chemical biology 11.12 (2015...

... doesn’t have a Math & Science Night (or some equivalent), here are some quick tips to get things going...feedback on my presentation, she said “it was such a valuable extension to our curriculum and offered engaging...

...While this methodology has proven to be incredibly valuable to scientists, there are some potential complications... Addgene.org Browse all CRISPR plasmids Find validated gRNAs Check out our CRISPR guide page ...

... what it takes to thrive at the company and add value, not just “do the job”. When they ask about your...point out how awesome we are, but there is also value in knowing and owning your transferrable skills ...

... of the tested PIPs. Many split FPs have been validated for BiFC (Kodama and Hu, 2012). Having a rough...that BiFC is irreversible — FP fragments become covalently bonded by associating with one another — which...