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We narrowed to 456 results for: val

Showing: 201 - 220 of 456 results

  1. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...common features of viral vector backbones; once validated these universal primers can be used to titer any...include defective particles. Studies have found that values can be 10-1000 fold higher than for functional ...

  3. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...spectrum of fluorescent proteins (FPs) has been an invaluable resource for studying the organization and function...detected by labor-intensive processes like PCR or by evaluating visual phenotypes. Improving C. elegans fluorescent...

  4. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...contaminant is Addgene plasmid #44362. Method validation, limitations, and future plans How did AAV-68544...purposely mixed another AAV vector with AAV-68544 to validate our method. We then extracted the DNA and processed...

  5. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...Forward genetics screens are a valuable part of the molecular biology toolbox to identify new target ...working with mice and slightly less red tape for approval processes. Additionally, while both AAV and lentivirus...

  7. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ... on to the next thing. If I made a mistake, I evaluated what lessons I had learned – call it “troubleshooting...Additional Resources I asked some colleagues to share valuable life lessons they have learned during their scientific...

  8. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...Each hit from the screen should be independently validated to ensure that the desired modification produces...place. With the proper experimental design and validation practices, CRISPR libraries can help you learn...

  9. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...making all the tools for plant research here and validating them, it made the most sense to us to enable ...women in science, and many other topics. What’s the value of engaging about science in that way for you? Patron...

  11. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ... what it takes to thrive at the company and add value, not just “do the job”. When they ask about your...point out how awesome we are, but there is also value in knowing and owning your transferrable skills ...

  12. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ... doesn’t have a Math & Science Night (or some equivalent), here are some quick tips to get things going...feedback on my presentation, she said “it was such a valuable extension to our curriculum and offered engaging...

  14. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ... of the journal club?” You also likely want to evaluate the “success” of the journal club. This can be...journal club’s usefulness should be continually evaluated and assessed for relevance.  Concluding thoughts...

  15. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...likely for adenoviral vectors – namely due to the removal of certain adenoviral genes that apparently contribute...36996871. Tatsis, N., Fitzgerald, J. C., Reyes-Sandoval, A., Harris-McCoy, K. C., Hensley, S. E., Zhou...

  16. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...whether you are planning your own ExM experiment or evaluating ExM images in a new publication.         Why .... 2015).  Despite these limitations, ExM is a valuable tool, especially when paired with complementary...

  17. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...Einstein might have put it best when he stated, “the value of an education . . . is not the learning of many...rejected, etc.), while reminding participants of the value of this approach and encouraging them to engage ...

  18. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ... to identify knock-in clones by reading up on validating genome edits to find screening strategies for... Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs for Your Next Experiment Check out our CRISPR...

  19. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...While this methodology has proven to be incredibly valuable to scientists, there are some potential complications... Addgene.org Browse all CRISPR plasmids Find validated gRNAs Check out our CRISPR guide page ...

  20. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...phosphopantetheinyltransferase) – These enzymes catalyze the covalent attachment of a fluorophore ligand to a peptide...and manipulating proteins in live cells through covalent labeling." Nature chemical biology 11.12 (2015...
Showing: 201 - 220 of 456 results