...to see if the type of science we do fits in with what they want to do. The experiments must fit with their...strive for a common big-picture goal. It is worth doing some some serious pre-work to find a lab that will...is this PI looking for in a new researcher?,” “How do I convince the PI to choose me?” We asked three leading...

...Although plasmids do not naturally exist in mammals, scientists can still reap the benefits of plasmid-based...transfection. Unlike bacterial transformation, scientsts do not "select" for cells that have taken up the plasmid...concentration for selection in mammalian cells. Before doing a transfection experiment, it is important to determine... and is not appropriate for mammalian selection – do not confuse this with G418 (aka Geneticin). Neomycin...

... are an expert networker (or if you just aren’t doing it at all yet) watch my webinar, “Not Networking...constructive suggestions and feedback. The best thing to do to make your mentor want to keep working with you...This was really helpful, perhaps you wouldn’t mind doing it again next month?” Some mentoring relationships...last for an hour or for a year. Some click and some do not. These are all normal outcomes. If you have a...

...Producing Your rAAV AAV Titers: Where Do They Come From and What Do They Mean? Overcoming the Challenges...viral production, particularly for labs that already do mammalian cell culture. HEK-293T cells are the most...application. With mammalian systems, it is standard to do only one passage (transfection). This differs from...and widely available. Unlike mammalian cells, they do not require serum or an incubator to grow. The virus...

...What do a viral vector production facility, food allergy testing lab, and the grad student down the hall...concentration of a target molecule in an unknown sample. To do this, you serially dilute a control sample of known...determine the concentration of the unknown sample.  How do I make the standards for a standard curve? You create...somewhere in the middle of the standard curve. If you do not know what concentration to expect, you may need... should be within 15% of the expected value. How do I plot and use a standard curve? To create a standard...

...’s not always easy to figure out what you want to do after graduate school, at least not while you’re ...your career goals, find people who are. I wanted to do fun science activities with the public, so I reached... interests! Especially as someone interested in doing science outreach, not only can I easily share science...graduate students because I wasn’t sure what I wanted to do. A few weeks into volunteering, I was talking to ...said, “Boy, I love science,” and I realized, I still do too! Beyond the outreach experience I gained from...

...while some might argue that the only way to do something is to do it huge, there are times when a more incremental...phenotypes, such as cell-non-autonomous effects, that do not lend themselves to screening in a pooled setting...

... Organizing your lab notebooks, protocols, and to do lists can be easier than you think! Here are three...spot the colors and text. Tip 2: Use electronic to-do lists to access your tasks on the go Never miss a...sync everything to your calendar. Ditch the paper To Do List and move to one that moves with you. Use software...

...types and deliver any cargo you fancy (size limits do apply, however.) Why use a virus? Viruses are essentially...viruses Adenoviruses infect non-dividing cells but they do not permanently integrate into the host genome (non-integrating...(non-integrating) as lenti and retro viruses do. They are generally used to achieve transient, high expression...adenovirus is their low immunogenicity, which means they do not stimulate a strong immune response when introduced...

... thing you’ll want to do is store it away safely. The first thing you should do is record the manufacturer...accidentally leave your antibody out on the bench overnight. Do not panic! Typically, antibodies are okay if they...however, is pretty much every other assay you can do. Most non-HRP conjugates are fluorophores, which ...