We narrowed to 783 results for: INA

...activation-induced cytidine deaminase (AID) and an engineered tRNA adenosine deaminase (TadA) to a catalytically...fusing catalytically inactivated RNA-targeting Cas13 (dCas13) with the adenine deaminase domain of ADAR2. ... by evolving the adenine deaminase domain of ADAR2 into a cytidine deaminase. This editor still retains...Cas9/gRNA ribonucleoprotein delivery Cytosine deaminase for RNA editing CRISPRi toolkit for Burkholderia...Find the gRNA and packaging plasmids   Cytosine deaminase for RNA editing Feng Zhang’s lab previously developed...

...multiplexed using tRNAs, within the lentiviral 3’ long terminal repeats (LTR). The 3’ LTR is duplicated during...the lentiviral insert was minimized to reduce recombination and avoid separation of the multiplexed elements...fold improvement in detection efficiency over the original CROPseq. Find CROPseq-multi plasmids here Walton...developed a new prime editor that incorporates La’s N-terminal domain, PE7 (Figure 2).  PE7 shows enhanced editing...-2 research   By Ashley Waldron Ready-to-use recombinant antibodies were recently added to the Addgene...Image from Addgene. These antibodies are recombinant versions of mouse monoclonal antibodies generated...2021). Check out their paper for details on the original antibodies. Find Anti-SARS-CoV-2 Antibodies here...

...homologous recombination efficiency to reduce the number of plants you need to grow to get a recombinant because...solicitation requires funded labs to rapidly disseminate protocols, methods, data, and tools that result...published, but to engage in ongoing, real-time dissemination of information and materials. Many groups publicized...labs all include materials sharing in their dissemination plans and in particular, that they all share...organismal systems described at this meeting were extraordinarily diverse. Plans to develop tools to study their...   Genus Microtus (Zoe Donaldson) Voles have fascinating social behavior. For example, two similar species...because they have only 1,700 genes total). Hydra (Celina Juliano) This is a known stem cell and regeneration...

...AAV particle. Finally, the precision of the assay can vary up to two fold for the final titer value, which... of 1:1 (Zeltner et al., 2010). However, for recombinant AAV2, the same study reported a physical-to-infectious...2010), while another reported that only 20% of recombinant AAV2 particles were full, compared to 50% of ...annealing can be affected by the presence of protein contaminants, which is a factor in AAV titration since the... be as important as the relative titer values. Finally, because physical titer does not give an indication...packaging of genomes can limit production of recombinant AAV-2." Gene therapy 6.7 (1999). PubMed PMID:... Lock, Martin, et al. "Characterization of a recombinant adeno-associated virus type 2 Reference Standard...

... at Mount Sinai Hospital, Toronto, Canada where he studies protein-serine/threonine kinases implicated...detect possible errors in assumptions, use of inappropriate statistical methods, omission of some data, ...tools and assumptions to reveal findings that the originator was oblivious to, no matter how much time they...productivity”, so this is unlikely. Usually, the originating scientist and colleagues are privileged in viewing...generators. This practice doesn’t appear to harm the originating researchers, even if they are occasionally beaten...data are likely, at least in part, due to our inadequate cataloguing of how results were obtained and ...multiple, complex panels. PLOS, itself, has not finalized its definition of necessary data requirements ...

...possibility of carrying over contaminants from the plasmid prep process into the final plasmid eluate. Possible...designed with environmental sustainability in mind (Table 2). Table 2: Sustainability features highlighted in...more importantly, the DNA needs to be free of contaminants, such as bacterial endotoxins, that could affect...Possible contaminants can sneak in from almost any step of the process and include things like genomic DNA...by excess material, leading to less DNA in your final eluate. Bead-based kits can be more flexible in ...impact, check out Addgene’s blog post on bringing sustainable practices for the lab. Plastic consumables (including...by more than half 70% recycled materials Sustainable forestry certification Starch-based compostable...

... its destination plasmids. In the destination plasmid, the ccdB gene is flanked by recombination sites...introduce a gene of interest into the destination plasmid, recombination sites must first be added to flank...compounds needed for growth, or auxotrophy, the inability to synthesize a compound needed to grow. Auxotrophy...usually something toxic. This gene is found on the original plasmid and either the insertion of a DNA fragment...flank the gene. Successful recombination and integration of the gene of interest at these sites in the plasmid...

...Minimizing off-target editing ABEs were originally developed from RNA deaminases, using directed evolution to get...DNA-targeting technology with a fused cytidine deaminase to create C→T base edits at a target locus. A ...characterized this RNA-editing activity and developed a deaminase variant with a V106W mutation, which reduced RNA...directed evolution, introducing mutations to the deaminase subunit to produce a series of ABE8s that were...editing. Base editing in the present—and future The final base editor that was administered to KJ was designated...J., Liquori, A. J., Murray, R., Packer, M. S., Rinaldi, C., Slaymaker, I. M., Yen, J., Young, L. E., &...sciadv.aax5717  Richter, M. F., Zhao, K. T., Eton, E., Lapinaite, A., Newby, G. A., Thuronyi, B. W., Wilson, C....

...and practicing my SciComm skills and I had to coordinate with other people for a variety of projects. ...conceptual chunks: prioritization, planning, and procrastination. Time management through prioritiziation Every... more time for that task the next time around. Finally, when prioritizing, you should always be aware ...Susanna’s post Upload Eric’s post and give it a final read Browse list of previous depositors from Boston...Eric before doing anything else. Preventing procrastination Even if you’ve done all the prioritizing and...and planning in the world, procrastination can easily prevent you from making progress on a task. For ...few things I’ve found useful to avoid such procrastination: Remove distractions: Probably the easiest...

...colonies, and screened for the desired modification. Finally, the positive clones are sequenced to confirm the...plasmid. Although it is commonly believed that the final products of the PCR reaction are pieces of double...recent data (see Xia et al., 2015) suggests that the final PCR products are pieces of linear, double stranded...These homologous ends recombine in vivo to give the final circular plasmid product pictured here. B) Screening... of DMSO to the PCR reaction (usually around 3% final concentration). The DMSO reduces secondary structures...using a methylation-dependent enzyme (DpnI) to eliminate the parent plasmid from the PCR products, the ...duplications (slightly larger bands sizes relative to the original template). Due to the small difference in size...