We narrowed to 714 results for: MAK

...a given phage will recognize on a host cell. To make matters even more interesting, much of what a phage...display little to no homology to anything else, making it difficult to quickly identify possible gene ...the T7 polymerase and a few proteases knocked out making them particularly useful for protein production...

...they are relatively small and easy to work with, making them the go-to choice for studying mammalian biology.... For editing the genome, the CRISPR/Cas system makes use of 3 components, a guide RNA (gRNA) of about...content in the mouse genome and the Cas9 enzyme will make a cut at the target site. This is when it gets exciting...

...our lab so we opted for AAV. While these benefits make AAVs useful for a wide variety of experiments, care...can still reduce AAV titer. For long term storage, make > 25 μl aliquots in 0.5 ml tubes, snap freeze in...humans and additional improvements are being made to make them even more useful for therapeutic purposes. ...

...ve been planning, it is a good idea to pause and make sure that your antibody has been appropriately validated...guarantee recognition of the target antigen. You want to make sure that your antibody binds to what you expect... epitope information for some antibodies, which makes it hard to ensure that you have unique antibodies...

...with DIO/FLEx vectors and off-target expression making it easier to determine the spread pattern around...your cells of interest. You should now be able to make smaller adjustments to a set of injections into ...K, Sanders E, Tasissa M, Kostman M, Tillgren M, Makana Hanley L, Mueller I, Mitsopoulos A, Fan M (2020...

...characterized by large DNA recognition sites, which makes them highly specific.  Jurassic Park by Michael ...   Star Trek: Into Darkness (2013) which is the remake of the space-melodrama Star Trek II: The Wrath ...has always served as a vision of the future and to make us reframe our perspective of the current. Although...

...characteristics to consider when selecting a dye or ligand to make your selection easier, as well as highlight some... imaging tools in biological research. This does make finding the right dye for your experiment more complicated..., M., Cincotti, G., Rampioni, G., Visca, P., & Kolmakov, K. (2023). Expanding the microbiologist toolbox...

...enjoying the party. There are so many teams that make Addgene functional, handling everything from facilities...this diverse group of talented individuals that makes Addgene — well, Addgene. We're so grateful for each...

...that we’ve learned in the lab that will hopefully make your life a little easier. These can range from ...Putting on lab gloves can sometimes be annoying. To make it easier, try this quick trick. Just flip, and ...

...get to talk to scientists from around the world making breakthrough discoveries!” I knew right then I ...way that a connected and collaborative community makes not just for a better day at the office but for ...

...samples from my assay at like 23:00, so I decided to make a giant snow angel for biologists in our building...performing routine lab maintenance tasks. Hot tip: make sure your PI/facilities are okay with the decorations...

... Other Retinopathies 14:39-25:59 : Strategies to Make Gene Therapies More General (more than correcting...Success 40:55-end : Professor Cepko's Thoughts on Making Scientific Information Accessible We'd like to...

...Expressing these chromoproteins in E. coli would make it easy to distinguish colonies from one another...always get a few isolated colonies. I should probably make it a bit more challenging, potentially by using ...

...wanted to take a week to celebrate the scientists who make a big impact in open science. Depositors consistently...this year, Addgene depositors quickly stepped up to make COVID-19 reagents available to researchers across...

...sometimes be elusive or have a certain mystique that makes them difficult to approach for the youngster just...people to do science? 16:08 - 22:04 : How have the make-up and scope of your graduate projects changed throughout...

...there had been no systematically established way of making an all-in-one vector for multiplex genome engineering...with a broader array of Cas9s, which they will also make available when they are validated and ready. “Currently...

...providing quality control and customer support, making plasmids easy for you to find and request in our... plasmids as of March 26, 2015. How does Addgene make it happen? Our awesome team of Addgenies! Our team...

...only viral protein present on the virion surface – making it critical for viral attachment and fusion. Once...  The EBOV glycoprotein’s role in infection also makes it a critical component of vaccines and the target...

...changes to our website! Part of sharing science and making access to materials easier is improving the tools...may be less obvious — but every change is meant to make your experience on our website better so you can...

...amoebasisters.tumblr.com/post/139944465237/lactose-around-we-can-make-some-enzymes-for   The take home message was that... that being creative and telling a story makes scientific topics both interesting and approachable!   ...

...able to communicate effectively is part of what makes working in a team productive. Take all the opportunities...or want to start a peer mentoring group? "What Makes a Good Mentor?" Form Your Own Peer Mentoring Group...

... by the Provider Limited liability Addgene makes it easy Even with all those benefits, we know that...auto-approve MTAs. Whatever your preference, we are here to make it fast and easy. Fast Approval: The UBMTA used...

...in the process. “We had several motivations for making GreenGate available through Addgene,” Lohmann said...system, the more building blocks will be available, making it easier for us to do our work.” References 1...

...Konermann said she is already aware of research groups making use of the new optogenetic tools in C. elegans ...neurons, she offers a tip: save yourself some time by making an AAV1 supernatant. (The details can be found ...

... your obscure growth medium, these apps can help make your lab life a bit easier. Since the post doesn...apps you’ve found useful in the comments section to make this post even more useful to your colleagues. Technique...

...eagerness to get, as well as give, information to make improvements. Members connect directly with one...card, afternoon off, etc.) Act on information to make change whenever possible. Be public about changes...

...and her lab members have noticed that the meetings make the lab run more smoothly. With a concrete plan ...Seeking Feedback Management for Scientists: What Makes a Good Manager Anyway Developing Transferable Skills...

...the last 10 years as well as the many people that make up the Addgene family. It was great to see the faces...Just as her grandfather used the printing press to make copies of the newspaper, Addgene uses E. coli bacteria...

...look, and a reorganization of existing content to make it more accessible. Other accessibility updates ...typically adopted by for-profit companies, while making use of our nonprofit status to achieve mission-...

... needed to go to an appointment at 3 PM, I could make up the hours I had missed at another time. This ...case for me at Addgene. There is a clear effort to make sure people feel connected.    As I became accustomed...