We narrowed to 435 results for: Trac

1. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

   Type

   Blog Post

   Published

   July 23, 2020, 1:20 p.m.

   ...Pages Other Links Check out Pawluk et al's Video Abstract Read up on All Things CRISPR with These Papers...

2. Engaging with science and society at pgEd

   Type

   Blog Post

   Published

   Sept. 20, 2018, 12:17 p.m.

   ...Find all of our science communication posts Extracurricular activities for a strong science career path...

3. Educational Planarian Tools for Teaching Developmental Biology Techniques

   Type

   Blog Post

   Published

   May 24, 2018, 1:20 p.m.

   ...these constructs, developmental biologists have to extract RNA from their organism of interest, use the RNA...

4. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

   Type

   Blog Post

   Published

   Dec. 14, 2021, 2:15 p.m.

   ...Charlton J, Mattei AL et al (2018). Genome-wide tracking of dCas9-methyltransferase footprints. Nat Commun...

5. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

   Type

   Blog Post

   Published

   Sept. 26, 2019, 12:50 p.m.

   ...components. However, this sometimes requires intracellular injections into the cytoplasm or pronucleus ...

6. Open Resources and Plasmid Tools For Studying C. elegans

   Type

   Blog Post

   Published

   July 18, 2019, 12:55 p.m.

   ...for all protein coding mRNAs, and annotations extracted from other databases (Mangone et al., 2008).  ...

7. Science communication: 9 Strategies to Get Your Foot in the Door

   Type

   Blog Post

   Published

   Sept. 27, 2017, 1:23 p.m.

   ...journalism graduate schools where you may take a science track. If you don’t want to commit time and money to a...

8. Fluorescent Proteins 101: Introduction to FRET

   Type

   Blog Post

   Published

   June 27, 2017, 1:03 p.m.

   ...Miyawaki et al., 1997), was designed to measure intracellular calcium and published in 1997. Since that time...

9. Plasmids for Endogenous Gene Tagging in Human Cells

   Type

   Blog Post

   Published

   April 6, 2017, 1:02 p.m.

   ...components of CRISPR/Cas9 (Cas9 protein and the crRNA-tracrRNA complex) and the donor plasmid into the cells ...

10. Plasmids 101: Modular Cloning

    Type

    Blog Post

    Published

    March 12, 2024, 1:15 p.m.

    ...lot of optimization - which is why MoClo is so attractive! It allows researchers to assemble and test many...

11. CRISPR in the Clinic

    Type

    Blog Post

    Published

    Nov. 2, 2023, 1:15 p.m.

    ...family) as part of a cocktail therapy for urinary tract infections. The Cas3 enzymes target several strains...

12. Viral Vectors 101: Chemogenetics

    Type

    Blog Post

    Published

    July 11, 2023, 1:15 p.m.

    ...nonnative ligands. However, they retain their intracellular interactions and still set off the same pathways...

13. Viral Vectors 101: Virus Safety

    Type

    Blog Post

    Published

    June 22, 2023, 1:15 p.m.

    ... and removed in a biosafety cabinet. For ultracentrifugation, heat sealed tubes can be used for extra ...

14. Lab Automation at Addgene

    Type

    Blog Post

    Published

    March 22, 2018, 1:28 p.m.

    ...from the Repository Trenches: Using Barcodes to Track Samples Resources at Addgene.org Find protocols...

15. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    Published

    March 8, 2017, 3:15 p.m.

    ...nourseothricin (clonNat), spectinomycin, streptomycin, tetracycline, combinations of the above, or other. High...

16. Tips for Technical Support Calls

    Type

    Blog Post

    Published

    Sept. 20, 2016, 2:39 p.m.

    ...experiment, but a picture of a gel or a sequencing trace file can go a long way in helping us understand ...

17. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type

    Blog Post

    Published

    Sept. 13, 2016, 2:30 p.m.

    ... on long-term efforts to better account for and track postdocs nationwide. On this level, FoR is currently...

18. Adapting Toehold Switches to Detect Zika Virus

    Type

    Blog Post

    Published

    Aug. 30, 2016, 2:30 p.m.

    ...global health emergency and called for the fast-tracked development of diagnostics. Mostly transmitted ...

19. Plasmids 101: Terminators and PolyA signals

    Type

    Blog Post

    Published

    March 31, 2016, 2:30 p.m.

    ...transcript followed by a weakly bound poly-uracil tract as shown in the figure to the right. The tertiary...

20. Gaining Leadership Skills Volunteering at a Professional Organization

    Type

    Blog Post

    Published

    Aug. 6, 2015, 2:30 p.m.

    ...engaged once you are in a leadership role or in a track towards one.  Many thanks to our guest blogger Juliet...

21. Experimenting in an Open Source lab: from CRISPR to Cats

    Type

    Blog Post

    Published

    July 23, 2015, 2:30 p.m.

    ...constantly moving so we need good ways to monitor and track them. We have a number of microscopes, including...

22. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type

    Blog Post

    Published

    Jan. 21, 2015, 1:30 p.m.

    ...or anger, because your discussion will get side-tracked, and you might create even more conflicts. 4) Define...

23. How-to: 5 Steps to a Great Panel Discussion

    Type

    Blog Post

    Published

    Oct. 22, 2014, 1:57 p.m.

    ...with it when this happens, but get things back on track if the direction of the interaction is not interesting...

24. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type

    Blog Post

    Published

    Dec. 10, 2020, 1 p.m.

    ...Beatty et al., 2012). These features make AdV an attractive candidate for in vivo delivery of advanced genome...

25. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type

    Blog Post

    Published

    July 2, 2020, 12:40 p.m.

    ...differentiation. Using a cell line model, the Xu Lab tracked enhancer-promoter interactions during erythroid...

26. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type

    Blog Post

    Published

    Oct. 30, 2018, 1:04 p.m.

    ...Beckmann, and W. Wurfel, Non-embryo-destructive Extraction of Pluripotent Embryonic Stem Cells: Implications...

27. The Scientific Conference Poster Session: Tips for Success

    Type

    Blog Post

    Published

    Aug. 29, 2019, 12:50 p.m.

    ...For most larger conferences, poster titles and abstracts are available online or in an app prior to the...

28. Tips to Make the Most of a Scientific Conference

    Type

    Blog Post

    Published

    May 23, 2019, 12:26 p.m.

    ...schedule. If there is a list of poster titles or abstracts available before the conference, comb through ...

29. The AAVantages of AAV in CRISPR Screens

    Type

    Blog Post

    Published

    Feb. 4, 2025, 2:15 p.m.

    ...expression of lentiviral vectors make them an attractive option. However, safety requirements and expression...

30. Magnetic Control of Proteins: More than a Dream

    Type

    Blog Post

    Published

    Sept. 24, 2024, 1:15 p.m.

    ...is a physicist with a background in optics and a track record of developing new microscopy tools for biological...