960 results

Learn about the csRNP epitope tagging method - a new and efficient way to tag endogenous genes using CRISPR.

June 28, 2018, 3:01 p.m.

Learn all about a highly processive reverse transcriptase, a new nanobody toolkit, and CRISPR-based tools for monitoring protein-DNA interactions

June 27, 2018, 1:16 p.m.

Congratulations to Paula Videira, Michael McMurray, & Richard Gronostajski who each are being awarded free plasmids form Addgene. Read on to learn how these researchers plan to use plasmids for research on diseases of glycosylation, arginine mutagenesis, & neural development.

June 26, 2018, 1:33 p.m.

Learn how to use the dTAG system to inducibly degrade your protein of interest.

June 21, 2018, 2:06 p.m.

Learn how you can use acoustic reporter genes for in vivo imaging experiments. These are good alternatives to fluorescence reporters.

June 19, 2018, 1:38 p.m.

Learn how to produce, purify, and titer adenoviral vectors for your research.

June 14, 2018, 11:44 a.m.

Addgenie Eric Perkins curates Addgene resources you can use in your chromatin research.

June 12, 2018, 11:40 a.m.

Learn about the various expression systems and plasmids you can use to produce your protein of interest.

June 7, 2018, 1:17 p.m.

Learn how rabies virus can be used to trace neuronal connections. AAV available at Addgene can help you with the process!

May 29, 2018, 1:51 p.m.

Learn how you can use planarian plasmids from the Sánchez lab to teach developmental biology techniques to new molecular biologists.

May 24, 2018, 1:20 p.m.

Learn how you can use plasmids to teach students about functional genomics with your own "Pathways Over Time" project.

May 22, 2018, 1:38 p.m.

Learn about a wide variety of potential solutions to reproducibility issues in the biological sciences including changes to scientific sharing, publishing practices, and experimental design.

May 18, 2018, 7:32 p.m.

Learn all about FPbase - a community editable fluorescent protein database.

May 16, 2018, 1 p.m.

Learn all about new optogentics tools, caffeine degradation, biosensors, and new fluorescent proteins.

May 15, 2018, 12:43 p.m.

Learn how you can use Synthego's ICE tool to analyze your CRISPR genome editing experiments.

May 8, 2018, 1 p.m.

Learn about new RNA-targeting Cas13d enzymes that average only 2.8 kb in size, are easy to package in low-capacity vectors, have high on-target and low off-target editing rates.

May 3, 2018, 1:48 p.m.

Learn how you can use split-BioID to study protein-protein interactions and build a better understand of your protein's interactome.

May 1, 2018, 1:57 p.m.

Learn all about plasmids for protein degradation, endosome biosensing, studying the epigenome, and controlling gene expression in yeast.

April 23, 2018, 2 p.m.

The final installment of our transferable skills guide. Learn how you can begin collaborating with people outside your organization and boost your projects with a mix of collaborators' skills.

April 19, 2018, 1:25 p.m.

Learn about the many ways you can deliver reprogramming factors to generate induced pluripotent stem cells (IPSCs).

April 17, 2018, 1:37 p.m.

In this episode of the Addgene podcast, learn what it was like to get Addgene off the ground and what the founders hope to see in the repository’s future.

April 13, 2018, 12:51 p.m.

Learn how you can use kits from the Arabidopsis Biological Resource Center (ABRC) to bring arabidopsis into the classroom.

April 12, 2018, 1:02 p.m.

Learn how RANbodies provide a scaffold for new, easy to detect, nanobodies.

April 10, 2018, 12:56 p.m.

Learn how to use Easi-CRISPR to generate mouse knock-in and conditional allele models.

April 5, 2018, 12:42 p.m.

Learn about xCas9, an engineering Cas9 variant with broad PAM flexibility and high on-target specificity.

March 28, 2018, 6:52 p.m.

Learn how you can use adeno-associated viruses (AAV) for genome editing - whether combined with nucleases or not. Also, read about the advantages and disadvantages of using AAV vs. CRISPR.

March 27, 2018, 1:32 p.m.

Learn some of the tools Addgene uses to achieve useful and affordable levels of lab automation.

March 22, 2018, 1:28 p.m.

Learn how to use Cas9 nickases for genome editing, including homology directed repair.

March 15, 2018, 12:59 p.m.

In this episode of the Addgene Podcast, learn all about Joachim Goedart and his work with fluorescent proteins at the University of Amsterdam.

March 9, 2018, 2 p.m.

Learn what a high fidelity DNA ligase is and when you might need one.

March 6, 2018, 1:49 p.m.

Learn why it's important to have high AAV titer for your experiments and find some tips to ensure high AAV titer.

March 1, 2018, 2:11 p.m.

Learn about the advantages of secondary nanobodies from the Gorlich lab over traditional antibodies. Applications include one-step fluorescent immunostaining.

Feb. 27, 2018, 2:04 p.m.

Addgene lab technician Racheal Komuhendo describes her recent experiences at a workshop at Lab Central and how you can get the most out of a similar workshop.

Feb. 20, 2018, 2:30 p.m.

Get quick lab tips from our new video series "Addgene Lab Tips"

Feb. 15, 2018, 2:41 p.m.

Resources to help improve precision genome editing through homology directed repair in experiments with CRISPR nucleases.

Feb. 14, 2018, 2:36 p.m.

Learn how you can use the CUT&RUN method to study protein-DNA interactions.

Feb. 13, 2018, 2:51 p.m.

Learn about the many fluorescent protein tools enabled by the nanoLuc luciferase.

Feb. 8, 2018, 12:17 p.m.

In this episode of the Addgene Podcast, learn all about research in Singapore and the genome engineering tools being developed in Wei Leong Chew's Lab.

Feb. 6, 2018, 2 p.m.

Learn about science writing in this post of our careers in science communication series.

Feb. 2, 2018, 3:20 p.m.

Learn how Addgene uses SnapGene to aid in plasmid quality control and check out some tools you might find useful for your own plasmid verification processes.

Feb. 1, 2018, 3:07 p.m.

Check out the 2017 winners of our #Deckthelab Contest

Jan. 31, 2018, 2:44 p.m.

Part five of our transferable skills guide: Learn the basics of managing team from Addgene Senior Research Scientist Karen Guerin.

Jan. 30, 2018, 2:42 p.m.

Learn how you can use a plasmid from the Rex lab at Vanderbilt to easily and selectively biotinylate your protein of interest.

Jan. 25, 2018, 2:09 p.m.

Use this transfection calculator when packaging your AAV vectors.

Jan. 23, 2018, 2:04 p.m.

Some promoters are only active under specific circumstances. Here's a guide to inducible promoters, which are regulated by chemicals, light, and/or temperature.

Jan. 18, 2018, 2:34 p.m.

Learn about the top requested AAV from 2017: pmSyn1-EBFP-CRE, a modular tool for expressing Cre-recombinase in neurons.

Jan. 17, 2018, 2:57 p.m.

Learn how you can visualize newly synthesized proteins with FUNCAT-PLA.

Jan. 16, 2018, 3:20 p.m.

Learn how you can identify your transferable skills and better prepare yourself for your next job interview.

Jan. 11, 2018, 3:29 p.m.

Learn all about our top requested plasmid from 2017 - lentivirus envelope vector pMD2.G encoding VSG-G.

Jan. 10, 2018, 4:13 p.m.

Check out all of the plasmids featured in our hot plasmids articles from 2017.

Jan. 9, 2018, 3:02 p.m.