We narrowed to 610 results for: STO;
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TypeBlog PostPublishedSept. 6, 2022, 1:15 p.m....complex biological reagents; many factors, like storage buffers and conditions (3,5,8), injection titers...
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MXS Chaining
TypeBlog PostPublishedFeb. 7, 2017, 3:30 p.m....Subcellular localization 1 TagBFP 399nm/ 456nm histone 2B (H2B) Chromatin 2 Cerulean 433nm/ 475nm Lyn-tag... -
Lentiviral Vector Uses and Overview
TypeBlog PostPublishedMay 19, 2016, 2:30 p.m....applications of lentiviral vectors! Lentiviral vector history and components In the early 1990s, researchers ... -
Plasmids 101: Colony PCR
TypeBlog PostPublishedMay 12, 2016, 2:30 p.m....cultures of your positive clones. If you want to store your clones longer term, just streak the colonies... -
CRISPR Antimicrobials
TypeBlog PostPublishedMay 3, 2016, 2:30 p.m....targeting a process essential to most bacteria, custom CRISPR antimicrobials can target specific sequences... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog PostPublishedMarch 17, 2016, 2:30 p.m....of Dr. Elke Mühlberger and Dr. Rachel Fearns at Boston University School of Medicine, Department of Microbiology... -
Negotiating Work and Life: How to Find the Joy
TypeBlog PostPublishedMarch 20, 2014, 3:04 p.m....always get done, but you’ll have time to read a story to your baby or run that extra two miles which is... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog PostPublishedJuly 13, 2023, 1:15 p.m....want to) make viral preps in your lab, there are custom viral prep services that can prepare the libraries... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog PostPublishedJuly 23, 2019, 12:46 p.m....the entry of pathogens to the CNS, but this also stops viral vectors, such as adeno-associated virus (AAV... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog PostPublishedJuly 2, 2019, 12:51 p.m.... visualizing neuronal differentiation by immunohistochemistry, it is not amenable to FACS. Thus, the ... -
Antibodies 101: Immunoprecipitation
TypeBlog PostPublishedDec. 7, 2021, 2:15 p.m....technique to identify DNA binding proteins involved in histone modification. Similarly, RNA IP (RIP) can be used... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog PostPublishedSept. 16, 2020, 1 p.m....post-injection. Livers from these mice were histologically normal and liver injury markers were not increased... -
Hot Plasmids - August 2020
TypeBlog PostPublishedAug. 18, 2020, 1:15 p.m.... localization signal or including fusions with Histone 2A or 2B can result in bright targeted expression... -
Lab to Office Culture Shock
TypeBlog PostPublishedFeb. 5, 2019, 1:04 p.m....cities around the world, such as BosLab here in Boston. Organizations like this one are always looking... -
CRISPR-mediated Plant Base Editors
TypeBlog PostPublishedJan. 3, 2019, 1:35 p.m.... this mutation by an adenine base editor would restore antibiotic resistance. To find such a protein, ... -
QC Sequencing Technologies at Addgene
TypeBlog PostPublishedJuly 15, 2025, 1:15 p.m....ddNTP) so that after its addition, DNA synthesis stops. The resulting DNA fragments are pooled and then... -
Plasmids 101: Shuttle Vectors
TypeBlog PostPublishedMay 20, 2025, 1:15 p.m....sequences are translated into amino acids. And I was astonished that the molecular language shared by every living... -
How to Be an Excellent Trainee
TypeBlog PostPublishedSept. 26, 2023, 1:15 p.m.... go ahead and write down where that one drug is stored or how to access the shared server. The blue box... -
Tips for Writing a Good Cover Letter
TypeBlog PostPublishedMay 21, 2024, 1:30 p.m....logistical, like briefly explaining a gap in your job history or sharing relocation plans. They can be skills-based... -
Plasmids 101: Broad Host Range Plasmids
TypeBlog PostPublishedApril 11, 2024, 1:15 p.m....properties that influence host range are not fully understood, but scientists have identified some of the features... -
Transferable Skills Guide: External Collaborations
TypeBlog PostPublishedApril 19, 2018, 1:25 p.m....keep it short. Soon, discussing with strangers stopped feeling weird and started to be fun. I just had... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog PostPublishedDec. 14, 2017, 2:08 p.m....presence of these two factors are not completely understood, the addition of an shRNA for P53 and replacing... -
Plasmids 101: Gateway Cloning
TypeBlog PostPublishedJan. 12, 2017, 3:30 p.m....elements (ribosome recognition sequences, start codon, stop codons, reading frame considerations, etc). This... -
Using Video to Share Your Science: We Share Science
TypeBlog PostPublishedNov. 3, 2016, 2:30 p.m....is not hard either. Here are some resources: ImpactStory, a website that helps researchers go beyond citation... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog PostPublishedOct. 25, 2016, 2:30 p.m.... all already have salaries at or above $50,000. Boston Children’s Hospital is the only part of the Harvard... -
CRISPR Activation: A Practical Guide
TypeBlog PostPublishedAug. 18, 2016, 2:30 p.m....plastic tool and has taken the scientific world by storm. While Cas9 has been most widely used to create ... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog PostPublishedOct. 1, 2015, 2:30 p.m....reinforcement are ICSS and place preference. Historically, ICSS was done by implanting an electrode and... -
Plasmids 101: E. coli Strains for Protein Expression
TypeBlog PostPublishedFeb. 10, 2015, 3:06 p.m....purification protocols are designed for soluble, cystosolic protein products, but this is not always achievable... -
CRISPR 101: Homology Directed Repair
TypeBlog PostPublishedJan. 26, 2023, 2:15 p.m....single-stranded DNA templates. Jacobi SA, et al. Poster at Keystone Precision Genome Engineering. 2017. Easi-CRISPR... -
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping
TypeBlog PostPublishedDec. 2, 2019, 2 p.m.... a split BirA* molecule that can reassemble to restore activity when fused to two interacting proteins... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog PostPublishedAug. 15, 2019, 12:30 p.m....postdoctoral researcher at Harvard. There’s an astounding number of ways to create chromosomal mutations... -
Antibodies 101: Introduction to Immunofluorescence
TypeBlog PostPublishedNov. 16, 2021, 2:15 p.m....with IF are immunocytochemistry (ICC) or immunohistochemistry (IHC). All three of these terms refer to... -
Hot Biosensors 2022: Year-End Roundup
TypeBlog PostPublishedDec. 6, 2022, 2:15 p.m....10.1021/acssensors.1c02201. LiLac sensor for lactate Stop and smell the flowers - or rather, the lactate! ... -
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog PostPublishedMay 13, 2025, 1:15 p.m....envelope plasmid typically encodes the vesicular stomatitis virus G protein (VSV-G), which facilitates viral... -
New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog PostPublishedSept. 28, 2023, 1:15 p.m....GTPase optogenetic systems (Berlew et al., 2022). Stopping traffic with CIB1-Rabs Instead of using an optogenetic... -
Adeno-associated Viruses (AAVs) for Genome Editing
TypeBlog PostPublishedMarch 27, 2018, 1:32 p.m....Although the mechanism of incorporation is not well understood, researchers have been using AAV vectors for ... -
Transferable Skills Guide: Time Management
TypeBlog PostPublishedDec. 5, 2017, 1:52 p.m....final read Browse list of previous depositors from Boston University Contact Dr. Gilmore directly to see ... -
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9
TypeBlog PostPublishedOct. 11, 2016, 2:30 p.m....CRISPR has taken the genome engineering world by storm owing to its ease of use and utility in a wide variety... -
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods
TypeBlog PostPublishedMarch 1, 2016, 3:30 p.m....) to name two more. Likewise, there are several store-bought cloning kits available which are all based... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog PostPublishedSept. 1, 2015, 2:30 p.m....laboratory research management consultant and coach in Boston, MA. He specializes in assisting research scientists... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog PostPublishedJune 1, 2023, 1:15 p.m....Kügler, S. (2017). A MicroRNA124 Target Sequence Restores Astrocyte Specificity of gfaABC1D-Driven Transgene... -
Is this the right place for me? 8 tactics for choosing a lab
TypeBlog PostPublishedOct. 2, 2018, 12:56 p.m....Symposium and #MeTooSTEM, academic bullies are hard to stop—they often have no boss or management structure ... -
Exploring Applications of the Bioluminescent HiBiT Tag
TypeBlog PostPublishedOct. 2, 2025, 1:15 p.m....protein activity. Bioluminescence detection is a cornerstone of modern cell-based assays and enables ultra-sensitive... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog PostPublishedFeb. 8, 2018, 12:17 p.m....furimazine by Promega’s Chemistry group is a good story (1). From a deep sea shrimp to a compact powerhouse... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog PostPublishedJuly 12, 2016, 2:30 p.m....Humanization (25-kbp) Created Using Embryonic Stem Cell/Blastocyst and CRISPR/Zygote Injection Approaches. Sci Rep... -
PiggyBac-ing Through the Genome Editing Field
TypeBlog PostPublishedMay 31, 2016, 3:30 p.m....re-cutting. Transposase removal of the cassette later restores the target sequence. This allows researchers to... -
Plasmids 101: Restriction Cloning
TypeBlog PostPublishedFeb. 18, 2016, 3:42 p.m....to covalently link the backbone and insert and, PRESTO, you have a full plasmid ready to be used in your... -
Deep Dive: Statistical Tests (Comparisons)
TypeBlog PostPublishedMarch 7, 2023, 2:15 p.m....distribution you are working with, graphing data with a histogram is sometimes sufficient to determine normality... -
Finding Your Science Policy Path
TypeBlog PostPublishedSept. 22, 2020, 1:15 p.m....move into science policy. Read broadly about the historical roots of science policy to connect to your why... -
Sequencing Options for CRISPR Genotyping
TypeBlog PostPublishedOct. 4, 2016, 2:30 p.m....Targeted amplicon sequencing doesn’t tell the whole story Figure 1: Schematic representations of the...