We narrowed to 292 results for: ADA
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TypeBlog PostPublishedJan. 13, 2015, 3:47 p.m....natural viral life-cycle, and researchers have adapted the components for use in genome manipulation. ...
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Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog PostPublishedAug. 20, 2019, 12:31 p.m....locomotion. Mice may need to be given some time to adapt to a testing apparatus. General stress levels of... -
Mouse Modeling, Part 1: Genetically Engineered Mice
TypeBlog PostPublishedJuly 11, 2019, 1:26 p.m....mouse models provide researchers with useful and adaptable tools to perform basic and preclinical research... -
Adenoviral Vector Production and Troubleshooting
TypeBlog PostPublishedJune 14, 2018, 11:44 a.m....which are essential for assembly of the virion. Adapted from Lee et al., Adenovirus-mediated gene delivery... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog PostPublishedMay 24, 2018, 1:20 p.m....triggers a cascade of events that results in the degradation of the target mRNA and silences its expression... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog PostPublishedDec. 14, 2021, 2:15 p.m....methylation, leaving the target CpG unmethylated. Adapted from Sapozhnikov and Szyf 2021. Let me leave... -
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)
TypeBlog PostPublishedAug. 24, 2021, 1:15 p.m....indirect ELISA (more details below) can also be adapted to a competitive format. Figure 2: Overview... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog PostPublishedApril 6, 2021, 12:30 p.m....library. This PCR step also adds the necessary adapter sequences for Illumina sequencing as well as unique... -
Hot Plasmids February 2024
TypeBlog PostPublishedFeb. 20, 2024, 2:45 p.m....by co-incubation of Cas-RNP and assist peptide. Adapted from Zhang et al. 2023 with permission. Find... -
Science communication: 9 Strategies to Get Your Foot in the Door
TypeBlog PostPublishedSept. 27, 2017, 1:23 p.m....to Zooniverse. The Natural History Museum has downloadable resources to enable you to set up your own citizen... -
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux
TypeBlog PostPublishedSept. 13, 2016, 2:30 p.m....Office Ben Corb - Director of Public Affairs at ASBMB Adam Fagen, PhD - Director, Early Career Scientist Segment... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog PostPublishedJuly 23, 2015, 2:30 p.m....Project One such space is the Victoria Makerspace in Canada, which I belong to. The team at the Victoria Makerspace... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog PostPublishedAug. 21, 2018, 12:31 p.m....Gateway cloning protocol is fairly flexible and adaptable to cloning different types of DNA, such as cDNA... -
Plasmids 101: Protein Expression
TypeBlog PostPublishedJune 7, 2018, 1:17 p.m.... grown in well-defined media and can be easily adapted to fermentation allowing for large-scale, stable... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog PostPublishedJune 22, 2017, 2:30 p.m....colocalization analysis example is given in Figure 2 (adapted from 4) - Dunn et al. 2011 provides an in-depth... -
Truncated gRNAs for Regulating Gene Expression
TypeBlog PostPublishedJan. 10, 2017, 3:37 p.m....interacts with a ≤15nt gRNA that contains an RNA adapter capable of recruiting a transcriptional activator... -
Plasmids 101: Knockout/Knock-In Plasmids
TypeBlog PostPublishedDec. 1, 2016, 3:30 p.m....them by depositing with Addgene! Figures were adapted from: Hall B, A Limaye, & AB Kulkarni. (2009) "... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog PostPublishedMay 18, 2021, 1:15 p.m....validating this method through monitoring GFP degradation, this protein compartment was used to optimize... -
Twenty Years of Addgene Sharing: CRISPR
TypeBlog PostPublishedApril 18, 2024, 1:15 p.m....our requestors. However, we can again see quick adaptation and steady use of all types of libraries, once... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog PostPublishedDec. 10, 2020, 1 p.m....through error-free homologous recombination (HR). Adapted from an image provided by Manuel Gonçalves; made...