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Addgene
Showing: 221 - 240 of 319 results
  1. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...expresses Cas9 from Streptococcus pyogenes with an invariant sgRNA scaffold and cloning sites for inserting...
  2. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...Hybridomas frequently express additional functional variable regions. mAbs 10:539–546. https://doi.org/10.1080...
  3. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...Yan et al., 2020). NHEJ can also be engaged by variants of the canonical Cas9 approach. A pair of CRISPR...
  4. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...animal derived product, there can be lot-to-lot variability in activity. No Genetic Tagging - Since antibodies...
  5. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...CRISPR Topic Page Read about High Fidelity CRISPR Variants Target Multiple Genes at Once with CRISPR Resources...
  6. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ... other orthologs. Ma et al. tested their dCas9 variants using gRNAs specific for telomeric sequences and...
  7. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ... methods for antibody conjugation can be quite variable from antibody to antibody or batch to batch. For...
  8. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...primary antibody without interfering with the hypervariable region, where an antibody binds to its target...
  9. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...‘core’ complex is composed of a tightly-bound invariant group of proteins, whereas the more loosely bound...
Showing: 221 - 240 of 319 results