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We narrowed to 535 results for: CAR

Showing: 221 - 240 of 535 results

  1. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...bacterial CRISPR targeting. Phage-derived vectors carry CRISPR RNAs (crRNAs) targeting either genomic (blue... integrating into the bacterial genome, and it carries CRISPR machinery targeting 1) the given resistance...hardest-to-kill bacteria. In using phage-derived vectors to carry CRISPR, we’re turning the tables on the bacteria...

  2. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...generating interesting drug-like compounds especially cardenolides which have been used to treat heart disease....disease. Caterpillars that eat milkweed generate cardenolides that tastes yucky (and is poisonous) for bird ...system (Receptor-Mediated Ovary Transduction of Cargo)  delivers Cas9 RNP to the arthropod germline by...

  3. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...vector can protect both plasmid-carrying bacteria and eukaryotes. But be careful — antibiotic mechanisms vary...and jump between all kingdoms of life. A bit of careful design will help make your experiment with shuttle...

  4. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...Cultures should only be passaged 20-30 times before discarding and thawing a fresh vial. In many labs, the history...Routinely test your cell line for mycoplasma and discard it if the culture has become overly confluent or...ensure high quality and reproducible results always care for your cell lines and virus properly and be consistent...

  5. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    Published
    Sept. 1, 2020, 1:15 p.m.
    ...’s which allow researchers to deliver oversized cargo (Hirsch et al., 2010). Today’s blog post will give... https://doi.org/10.1016/j.celrep.2017.06.064  Carvalho LS, Turunen HT, Wassmer SJ, Luna-Velez MV, Xiao...transduction following recombination between AAV vectors carrying parts of a larger gene. Nat Biotechnol 20:697–...

  6. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ... a western blot — and why you might want to be careful relying on so called “housekeeping proteins.” Fully...approach is so common that we’ve used it for our cartoon gels so far. However, it throws both assumptions...Convenient! Comparison of loading control approaches Cartoons are nice and all, but we’ll leave you with this...

  7. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...in vitro translation system. The gene set was carefully chosen to perform statistically relevant benchmarking...conveniently performed through the N-terminal eGFP tag carried on all constructs. The Cell-Free Protein Expression... 48 plasmid adapter library, with each plasmid carrying a unique barcoded Illumina-M13F bridging primer...

  8. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...latter would likely be more effective, so long as care is taken in the interpretation of results by requiring...use of CRISPR technology will always depend on careful experimental design, execution, and analysis. ... P-Y, Daley G, Church G (2013) Optimization of scarless human stem cell genome editing. Nucleic Acids ...

  9. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    Published
    Oct. 2, 2025, 1:15 p.m.
    ...double-stranded RNA. They hypothesized that if ADAR1 were carried by extracellular vesicles (EVs), edited transcripts... as a sensitive reporter for tracking proteomic cargo flow via EVs. Find ADAR-HiBiT Fusion Vector at Addgene...the heterogeneity and spread of hepatocellular carcinoma in human tissue and in vitro. Oncology Reports...

  10. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...Puschnik, A. S., Nagamine, C. M., Cheng, F., Qiu, J., Carette, J. E., & Vandenberghe, L. H. (2018). An Alternate... B., Fong, D. M., Mouravlev, A., Young, D., & O’Carroll, S. J. (2019). Astrocyte-selective AAV gene therapy....2014.00076 Nieuwenhuis, B., Haenzi, B., Hilton, S., Carnicer-Lombarte, A., Hobo, B., Verhaagen, J., & Fawcett...

  11. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...al., 2009), and at high copy numbers (Williams, Carnes, and Hodgson, 2009). More research is ongoing to...0092-8674(84)90060-6. PMID: 6323019. Williams JA, Carnes AE, Hodgson CP. Plasmid DNA vaccine vector design... JA, Luke J, Langtry S, Anderson S, Hodgson CP, Carnes AE. Generic plasmid DNA production platform incorporating...

  12. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...health sector, phages represent a reservoir of carbohydrate binding proteins waiting to be explored and ...spot this protein directly onto nitrocellulose and carry out the steps from blocking the membrane to probing...bacteriophage-encoded glycan binding proteins and carbohydrates." Current opinion in structural biology 34 (...

  13. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...population (Burger, C. 2004, Aschauer, DF. 2013, O’Carroll, SJ. 2021). This is due to differences in the ...Pillay S, Puschnik AS, Nagamine CM, Cheng F, Qiu J, Carette JE, Vandenberghe LH (2018) An Alternate Route for... 12. https://doi.org/10.3389/fnsyn.2020.00006 O’Carroll SJ, Cook WH, Young D (2021) AAV Targeting of Glial...

  14. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ...lesser than the mean of a control group. If you only care if one group is different in a certain direction...rate by only performing the comparisons you really care about. Family-wise error is the probability of a...Groups with Ordinal Dependent Variables: A Monte Carlo Simulation Study. Front. Appl. Math. Stat. Volume...

  15. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ... compartments in in vivo model systems, so extra care must be taken to assess if proper staining is achieved... such as conjugation with proteins, should be carefully managed. Proper dye-to-protein ratios, or the ... and quenching, which can be mitigated through careful selection and optimization. Fluorescent experiment...

  16. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ... interpreting your data therefore necessitates careful design of RNAi sequences, confirmation of RNA knockdown...doi.org/10.7554/eLife.48363 Uhlen, M., Bandrowski, A., Carr, S., Edwards, A., Ellenberg, J., Lundberg, E., Rimm...., Weston, C. A., Castells, E., Bowman, V. W., McCarthy, J., Amrani, Y., Duffy, S. M., Bradding, P., &...

  17. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...Vectors Available at Addgene: Lab Plasmids Dan Carlson RCIscript-GoldyTALEN, pC-GoldyTALEN Tom Ellis...target cells. The piggybac system has a much larger cargo capacity than lentivirus and is capable of efficiently...there are some drawbacks with this technique. Great care needs to be taken with common S288c strains since...apicomplexan genes remain uncharacterized. Recent studies carried out by Sebastian Lourido and colleagues constitute...

  18. Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More

    Type
    Blog Post
    Published
    Sept. 8, 2017, 7:52 p.m.
    ...Listen to Our Segment on mCyRFP1 Hot Plasmid 4: mScarlet - a new, bright, and monmeric red fluorescent ...fluorescent protein.  Listen to Our Segment on mScarlet Special thanks to our sound engineer/producer, Eddy Page...

  19. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    Published
    Dec. 5, 2023, 2:15 p.m.
    ...Codon Guide to Synthetic BiologyAuthor: Niko McCartyAntibodies 101: Conventional vs. Flow Spectrometry Author...Faculty Position Author: Erik Lee SnappSharing is Caring, But How to Distribute Open Hardware? Author: Benedict...
Showing: 221 - 240 of 535 results