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Showing: 221 - 240 of 553 results

  1. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ...Medicine have also recently described how the glycoprotein transforms itself to escape vesicles within host ...It’s pretty hard to escape news and fears of Ebola these days, as the infectious disease once limited...

  2. Download Science-Themed Zoom Backgrounds from Addgene

    Type
    Blog Post
    Published
    April 22, 2021, 1:15 p.m.
    ... interested in our fluorescent protein background. Download the orange fluorescent protein background....If not, you might want to check out Addgene’s Fluorescent Proteins blog topic to learn more about FRET ...

  3. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...fluorogenic primers Low Single self-quenching fluorescent molecule connected to a primer  No Yes ...normalize for that. Passive reference dyes are inert fluorescent dyes that are not dependent on amplification ... It is possible to multiplex qPCR when using fluorescent probe-based chemistry - theoretically, up to ... Tip🔥 The ABI machines automatically record fluorescent data on all channels - helpful if you forget ...

  4. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    Published
    April 27, 2017, 2:30 p.m.
    ... discusses some of the programs SITN runs and describes some of the more popular topics from the Science...telling. Chapter 5: The SITN Blog - Vini and Amy describe how the SITN blog is a source of scientific information...underwater? Chapter 8: Work-Life Balance - Vini describes how she balances working in the lab and working...

  5. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ...citrea scarless editing plasmids Sheng Yang’s lab describes a two-plasmid system that combines recombineering...plasmid tools that can be used in this way. As described in the above CRISPRi section, the Marraffini Lab... aeruginosa, Pseudomonas putida, Pseudomonas fluorescens, and Pseudomonas syringae. These vectors contain...system for CRISPR-Cas9-assisted genome editing in Escherichia coli. Acta Biochimica et Biophysica Sinica. https...

  6. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...plasmid with its elements described below.   Vector Element Description Origin of Replication ...quantity inside a cell Plasmids 101: Green Fluorescent Protein (GFP) Plasmids 101: Luciferase Monitor...

  7. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ...Each trap uses a slightly different approach (described in detail below), but fundamentally the reporter...of interest in any way. For example, if green fluorescent protein (GFP) was the reporter gene and green...construct can be something phenotypic (such as a fluorescent protein or something species-specific) or a selectable...

  8. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    Published
    Feb. 16, 2021, 2:15 p.m.
    ...a job search website like Indeed. Do the job descriptions sound interesting? Is the work something you... types of jobs people with master's have Job descriptions often fail to capture all of the responsibilities...responsibilities of a role, so it helps to see how people describe their actual work. To do this, try looking at ...

  9. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    Published
    Jan. 15, 2015, 1:50 p.m.
    ...fly fishing, and local grizzly bear attacks. He described one such recent attack as "hilarious". Welcome... Mehmet Fatih Bolukbasi from Scot Wolfe's lab described a chimeric Cas9 that is improving the nuclease...also got to show off our new CRISPR page (which describes how to plan your CRISPR experiment for our new...

  10. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...find the vector that fits your experiments, I've described below some of the most frequently requested vectors...transfected cells. The addition of an in-frame fluorescent fusion protein will allow you to visualize YGOI...that carries either a wild-type version of YGOI to rescue a mutated strain or a mutated version of YGOI to...

  11. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...CRISPR/Cas inspired genome editing was initially described by Cong et al. and Mali et al. in January 2013...Nine months later, more than 1500 publications describe work to improve the tool’s specificity, orthogonality...orthogonality, and multiplexibility in various species and describe an assortment of applications. Despite the ubiquity...

  12. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    Published
    April 23, 2020, 1:15 p.m.
    ...our AAV preparations. We recently published a description of the VGS process and use cases in Human Gene...ligation and tagmentation preparation approaches described previously require a preliminary double stranding...signature sequences in the data. Unlike previously described thermodynamics-based methods of serotype confirmation...

  13. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...instability-prone lentiviral vector plasmid in Escherichia coli Stbl3. 3 Biotech 3:61–70. https://doi.org...lentiviral expression vectors using reduced-genomeEscherichia coli. BioTechniques 43:466–470. https://doi.org...cloning strategy for efficient genome editing in Escherichia coli. Microb Cell Fact 19. https://doi.org/10.1186...

  14. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    Published
    Sept. 21, 2023, 1:15 p.m.
    ...immunocytochemistry (ICC). Not all of the solutions described here may be as effective for other techniques,...Technique Adaptable to Biotin-Free Reagents, Immunofluorescence, and Multiple Antibody Staining. J Histochem...Addgene blog Antibodies 101: Introduction to Immunofluorescence Antibodies 101: Affinity Reagents Antibodies...

  15. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ... light responsive than fluorescent proteins required to get a good fluorescent signal. It may not be necessary...are often necessary to visualize expression of fluorescent proteins, for example, as many viral particles...

  16. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    Published
    Nov. 20, 2018, 8:17 p.m.
    ... lacks context unless it links to a verified description of that reagent online. Furthermore, if the link... link between the catalog number and reagent description is lost (e.g. a supplier is bought out by another...specific information about the plasmid: name, description, how the plasmid was generated, validated sequence...

  17. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    Published
    Dec. 10, 2019, 1:58 p.m.
    ...Genetically encoded voltage indicators (GEVIs) change fluorescence in real time when neurons or muscle cells transmit...However, technical difficulties such as small fluorescence changes or a requirement for very high excitation...sensors can detect action potentials in worms with fluorescence changes of up to 128% per 100 mV in the pharynx...

  18. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...bodies decorated with a fluorescent protein remain functional. Attaching the fluorescent protein fusions allowed... body dynamics. Second, we showed that these fluorescent R bodies can rupture E. coli spheroplasts (cells...

  19. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...Refactoring and optimization of light-switchable Escherichia coli two-component systems. Schmidl SR, Sheth... at Addgene. Synthetic biology: engineering Escherichia coli to see light. Levskaya A, Chevalier AA, ...Multichromatic control of gene expression in Escherichia coli. Tabor JJ, Levskaya A, Voigt CA. J Mol Biol...

  20. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    Published
    July 28, 2017, 1:30 p.m.
    ...Harvard University. In my previous two posts, I’ve described the fundamentals of how to work with Drosophila...Drosophila as an experimental model organism. I then described the Gal4/UAS system used by geneticists to study...manipulations. You can produce some very beautiful immunofluorescent images like in this paper (2). Metabolic assays...
Showing: 221 - 240 of 553 results