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We narrowed to 680 results for: Eng;

Showing: 221 - 240 of 680 results

  1. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...may provide a method for doing just that. While challenges remain in the delivery of these agents, CRISPR...potential clinical applications, Ando et al. recently engineered synthetic phages, each based on the well-studied... Lemire, Diana P. Pires, and Timothy K. Lu. “Engineering Modular Viral Scaffolds for Targeted Bacterial...Addgene Blog CRISPR Methods for Bacterial Genome Engineering Treating Muscular Dystrophy with CRISPR Gene ...

  2. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...the level of target antigen in a sample. While Engvall et al. originally developed the ELISA assay to ...small molecules from a variety of sample types (Engvall 1971). Scientists value ELISAs for their accuracy...antibodies. Assay development, however, can be challenging since scientists must first identify two independent...Longer protocolIncreased chance of cross-reactivityChallenging to develop assay Competitive Appropriate...

  3. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ... Rett Syndrome, respectively. One of the main challenges facing Rett researchers is how to deliver the...depositors David Liu, PhD, Keith Joung, PhD, and Feng Zhang, PhD, is pursuing CRISPR/Cas9 technology for... by Gail Mandel. There are numerous technical challenges to overcome for both of these approaches, such...abstract Sinnamon JR, Kim SY, Fisk JR, Song Z, Nakai H, Jeng S, McWeeney SK, Mandel G (2020) In Vivo Repair of...

  4. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ...for a liver transplant. My lab will combine bioengineered scaffolds with patient stem cells to generate... in your chalk talk, there's the vital task of engaging your audience. You need to make your audience ...search committees. If possible, do at least two full length practices (~1 h) and get feedback. It’s also helpful...outside your field. If they can understand and are engaged by your presentation, then you’re on the right ...

  5. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...to an adenine base editor. A massive protein engineering effort by Gaudelli et al. resulted in the successful...colonies and subsequent rounds of evolution and engineering gave rise to a mutant TadA (TadA*) that satisfactorily.... Kang, Beum-Chang, et al. "Precision genome engineering through adenine base editing in plants." Nature...CRISPR-mediated base editing in rice. In Genome Engineering: The CRISPR-Cas Revolution, Cold Spring Harbor...

  6. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...libraries. The Genome Engineering using CRISPR/Cas Systems Forum- Moderated by the Feng Zhang lab, this is...presentation deceive you; pooled libraries can be quite challenging to use! In this post, I’ll walk you through how...activation screening - An excellent protocol paper by the Feng Zhang lab describing construction, lentiviral production...

  7. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...amounts of target proteins or antibody bonding strengths.    . Steps of an IP: 1) Protein extracts...Protein G have an affinity for immunoglobulins, the strength of the interaction varies between antibody host...many factors, including salt concentration, ionic strength and pH, affect binding the lysis buffer may need...step. The wash buffer has an optimal pH and ionic strength that breaks weak interactions from residual non-specific...

  8. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...double strand break repair, it has proven more challenging to develop high efficiency CRISPR-based approaches...Howes, Rob, and Christine Schofield. "Genome engineering using adeno-associated virus (AAV)." Chromosomal...Benjamin E., and David V. Schaffer. "Combining engineered nucleases with adeno-associated viral vectors...editing." Precision Medicine, CRISPR, and Genome Engineering. Springer, Cham, 2017. 29-42. PubMed PMID: 29130152...

  9. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...16568824. Hmelo, Laura R., et al. "Precision-engineering the Pseudomonas aeruginosa genome with two-step...PMID: 31201277. Liljeruhm, Josefine, et al. "Engineering a palette of eukaryotic chromoproteins for bacterial...bacterial synthetic biology." Journal of biological engineering 12.1 (2018): 8. PubMed PMID: 29760772. PubMed...Thomason, Lynn C., et al. "Recombineering: genetic engineering in bacteria using homologous recombination." ...

  10. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    Published
    April 9, 2019, 1:13 p.m.
    ...sequence in the way that they like, the design and engineering of fusion proteins is relatively straightforward...properties of the protein of interest can be challenging. In this blog I discuss strategies to generate...before generating a fusion protein, e.g. linker length, the specific fluorescent protein variant, and ... (catalytic activity or interactions) can be challenging. If a fusion can complement a knock-down or knock-out...

  11. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...for investigating neuronal signaling and for engineering improved sensors for these and other targets....this in mind, Ed Boyden and colleagues set out to engineer a potassium ion biosensor with a binding affinity...sensors. Using genome mining and structure-guided engineering of potassium binding proteins, they developed...and are a promising starting point for further engineering. Figure 5: Structure-guided design and genome...

  12. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...natural inclination for this skill that you could strengthen. The right set of transferable skills, coupled...lack thereof): Information sharing gets more challenging the bigger an organization gets, and creating...conflict resolution is likely not one of your strengths. Empathizing with people, especially people with...recognize that conflict resolution may never be a strength for you. But if you are a problem solver, and ...

  13. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ... validated first through the production of full-length protein (the TAG codon should encode phosphoserine... instead of a stop codon, which will allow full length GFP to be translated), and second, through a gel...for parameters such as induction temperature and length of induction, which will vary between different...phosphorserine has actually been incorporated into the full-length protein. Up to 80% p-Ser incorporation into E17TAG...

  14. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 2:15 p.m.
    ...on. In addition, researchers have created many engineered serotypes by mixing, matching, and modifying ...summarize briefly: AAVrg is derived from AAV2 and engineered to infect neurons and undergo retrograde transport...Nagarajah, R., Vitale, J., Wong, A. C. H., Gokal, D., Feng, Y., Tabar, M. S., Metierre, C., Parsania, C., Song...W., Liu, X., Cai, Y., Kou, J., Wang, J., Li, L., Peng, S., Xu, Z., Zhang, W., Qiu, Y., Wu, Y., Ye, C.,...

  15. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ... genomic DNA to amplify the gRNA targets was challenging because you’re limited by the sequence of the...straightforward, and is a testament to how far genome engineering has come. For comparison, in 2007 it took about...lentiCas9-Blast (Plasmid #52962) was a gift from Feng Zhang and is described in Improved vectors and genome-wide... genome engineering...

  16. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...Today's postdoctoral fellows (PFs) face a number of challenges ranging from long periods of training to limited...for a successful postdoc fellowship. 1. Reverse engineer success On Day 1 of your postdoctoral experience...data needed to rule out alternative models or to strengthen your new model?  PFs need to be regularly thinking...and answering questions. Organizing a thoughtful engaging talk takes significant effort. It requires evaluation...

  17. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    Published
    Oct. 17, 2019, 12:28 p.m.
    ...possible by the light: when hit with the correct wavelength, microbial rhodopsins enable ions to flow across...a laser. The laser flashes light of specific wavelengths, selectively turning neuron activity on or off...1977): 237-243. PubMed PMID: 20882. Mei, Yuan, and Feng Zhang. "Molecular tools and approaches for optogenetics...28772120. PubMed Central PMCID: PMC5830081. Zhang, Feng, et al. "The microbial opsin family of optogenetic...

  18. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...selection. Knock-in events tend to be rare, and the challenge with any experiment is in efficient recovery of...Toolkit and SapTrap-SEC kit for C. elegans genome engineering have also facilitated CRISPR editing in C. elegans...Higgins CD, Goldstein B (2015) Streamlined Genome Engineering with a Self-Excising Drug Selection Cassette.....113.156075 Ward JD (2014) Rapid and Precise Engineering of theCaenorhabditis elegansGenome with Lethal...

  19. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ... cytokines like TGFβ and IL-6. However, when strengthened through efforts to boost the activity of the... form of immunotherapy in recent times. These engineered T cells are capable of recognizing targets like...the above therapeutics, techniques like genome engineering have played a huge role. For instance, researchers...at Binghamton University. She works on genome engineering and cancer immunotherapy, and is interested in...

  20. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...-1 Wang R, Xiang S, Feng Y, Srinivas S, Zhang Y, Lin M, Wang S (2013) Engineering production of functional...are between 5-8 amino acids long, with a typical length of 5 or 6 amino acids (Cruse et al., 2004). Because...natural immune system production and many more engineered methods. Each type of antibody and production...
Showing: 221 - 240 of 680 results