We narrowed to 533 results for: Erf

...have its limitations, especially when trying to perform high-throughput experiments. If a user wants to... modified to tag hundreds of genes, generating powerful genetic screening libraries. Additional protein...

...necessarily represent the views of Addgene. Addgene performs Sanger sequencing on select regions of all distributed...plasmids as part of quality control, but does not perform functional tests. The newest genome engineer sharing...

...from organism to organism. MMEJ doesn’t yield a “perfect repair” like HDR, but it’s much more predictable...ready-made PITCh plasmids available from Addgene are perfect for expressing GFP from a given promoter, and the...

...discuss how CRISPR libraries are being used to perform genome-wide screens and highlight some of the reagents...What are the steps involved in a CRISPR screen? Performing a forward genetic screen using CRISPR libraries...

...belongs to the penicillin family of antibiotics. It interferes with cell wall synthesis as its mode of action...Plasmids 101: Blue-White Screening Choosing Your Perfect Plasmid Backbone  ...

... when they begin flowering. It is important to perform the transformation not long after flowering begins...2007) ‘Improved Gateway Binary Vectors: High-Performance Vectors for Creation of Fusion Constructs in ...

...discusses some of the key things to consider when performing colony PCR.   Designing colony PCR primers ...Choosing a Molecular Cloning Technique Learn to Perform a Bacterial Transformation Deposit Your Positive...

...measured to quantify (indirectly) transcription performed by the viral polymerase. Alternatively, the mRNA...independently. In addition, experiments can be performed in the absence of some viral proteins (compared...

...information. Although two-hybrid systems are a powerful tool for screening molecular interactions, accuracy... and reproducibility are common problems when performing these experiments. False positives can occur ...

...wavelength (yellow derivatives) A206K Interferes with dimer interface (monomeric derivatives) K26R, Q80R...

... review click here). Alternative envelopes are powerful tools as they allow researchers to target transduction...with respect to the specific experiments being performed and you should understand the guidelines for using...

...C-terminus (or even in the middle of proteins) and performs well in many different antibody-based applications...high rate of spontaneous antibiotic resistance interfering with selection. Using an inducible Cas9 expression...

... subsequently reproduce the research. A third, powerful example is the integration with protocols.io, ...DSpace repository. These integrations utilize an interface that enables users to conveniently select and ...

.... These variants use different RT enzymes and performed large edits with similar or better efficiency ...we began distribution and has been verified to perform well in standard IHC and western blot protocols...

...standard FPs. The Looger Lab was thus able to easily perform a four-color labeling experiment using smFPs and...References 1. Viswanathan, Sarada, et al. "High-performance probes for light and electron microscopy." Nature...

... the ICE tool by analyzing thousands of edits performed over multiple experiments and comparing the robustness...search for a particular sequence or name. You can perform more in-depth analyses on each sample by clicking...

...Katrin Michel. Cre-lox is an incredibly popular and powerful site specific recombinase (SSR) system, but it...Buchholz, Angrand, and Stewart 1998). FLPe’s performance was further improved, by adding a nuclear localization...

...chemotherapy (HIPEC). With this technique, surgery is performed to remove the mesothelioma tumors from the peritoneum...to which it may have spread. Then, the surgeon performs a heated chemotherapy wash throughout the abdominal...

...which ones aren't?     This video shows how to perform a Kirby-Bauer disk diffusion test and check bacteria...testing for antibiotic resistance isn’t commonly performed because resistance in these infections is so rare...

...pressure to maintain consistently high levels of performance, and occasionally being faced with “shifting ...crucial to how our clients’ products will end up performing in the market. Hence, as a PhD, an MD, or someone...