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  1. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...belongs to the penicillin family of antibiotics. It interferes with cell wall synthesis as its mode of action...Plasmids 101: Blue-White Screening Choosing Your Perfect Plasmid Backbone  ...

  2. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...discusses some of the key things to consider when performing colony PCR.   Designing colony PCR primers ...Choosing a Molecular Cloning Technique Learn to Perform a Bacterial Transformation Deposit Your Positive...

  4. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...information. Although two-hybrid systems are a powerful tool for screening molecular interactions, accuracy... and reproducibility are common problems when performing these experiments. False positives can occur ...

  6. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ... review click here). Alternative envelopes are powerful tools as they allow researchers to target transduction...with respect to the specific experiments being performed and you should understand the guidelines for using...

  7. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ... the ICE tool by analyzing thousands of edits performed over multiple experiments and comparing the robustness...search for a particular sequence or name. You can perform more in-depth analyses on each sample by clicking...

  8. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...Katrin Michel. Cre-lox is an incredibly popular and powerful site specific recombinase (SSR) system, but it...Buchholz, Angrand, and Stewart 1998). FLPe’s performance was further improved, by adding a nuclear localization...

  9. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ... subsequently reproduce the research. A third, powerful example is the integration with protocols.io, ...DSpace repository. These integrations utilize an interface that enables users to conveniently select and ...

  10. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...chemotherapy (HIPEC). With this technique, surgery is performed to remove the mesothelioma tumors from the peritoneum...to which it may have spread. Then, the surgeon performs a heated chemotherapy wash throughout the abdominal...

  11. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...which ones aren't?     This video shows how to perform a Kirby-Bauer disk diffusion test and check bacteria...testing for antibiotic resistance isn’t commonly performed because resistance in these infections is so rare...

  12. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...pressure to maintain consistently high levels of performance, and occasionally being faced with “shifting ...crucial to how our clients’ products will end up performing in the market. Hence, as a PhD, an MD, or someone...

  13. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...C-terminus (or even in the middle of proteins) and performs well in many different antibody-based applications...high rate of spontaneous antibiotic resistance interfering with selection. Using an inducible Cas9 expression...

  14. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    .... These variants use different RT enzymes and performed large edits with similar or better efficiency ...we began distribution and has been verified to perform well in standard IHC and western blot protocols...

  15. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...standard FPs. The Looger Lab was thus able to easily perform a four-color labeling experiment using smFPs and...References 1. Viswanathan, Sarada, et al. "High-performance probes for light and electron microscopy." Nature...

  16. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...on the fly”. This becomes intuitive with touch interfaces where the viewer is more likely to want to manipulate... to Research Data? - by Francine Berman and Vint Cerf (Science, August 2013) PLOS’ Bold Data Policy - ...

  18. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ... can also be easily knocked-down through RNA interference (RNAi) by feeding the worm bacteria expressing... Andrew, et al. "Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans...

  19. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...molecular processes that make aptamer biology a powerful tool. In this post, we’ll take a deeper dive into... Matthew D. Disney, and Samie R. Jaffrey. "A superfolding Spinach2 reveals the dynamic nature of trinucleotide...
Showing: 221 - 240 of 520 results