We narrowed to 262 results for: INO
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TypeBlog PostPublishedOct. 13, 2022, 1:45 p.m....introduce a single nucleotide modification at a defined amino acid to mimic a patient mutation or maybe to disrupt...
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Antibodies 101: Reading a Flow Plot
TypeBlog PostPublishedFeb. 6, 2024, 3:13 p.m....Seroogy, C. (2017). Neonatal immune response to rhinovirus A16 has diminished dendritic cell function and... -
CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog PostPublishedApril 13, 2021, 1:15 p.m....temperature-sensitive replicon for self-curing, an arabinose inducible lambda-red gene, and an IPTG-inducible... -
Plasmids 101: Plasmid Incompatibility
TypeBlog PostPublishedMarch 31, 2020, 1:15 p.m....References del Solar G, Giraldo R, Ruiz-Echevarría MJ, Espinosa M, Díaz-Orejas R (1998) Replication and Control... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog PostPublishedNov. 1, 2018, 12:35 p.m....PMID: 19678844. PubMed Central PMCID: PMC2906245. Danino, Tal, et al. "Programmable probiotics for detection... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog PostPublishedDec. 14, 2017, 2:08 p.m....iPSC programming spanning from fibroblasts and keratinocytes to peripheral blood mononuclear cells (PBMCs... -
The Challenges of Cell Culture
TypeBlog PostPublishedMay 5, 2016, 2:30 p.m....Resistance and Metastatic Potential in Adenoid Cystic Carcinoma, Up-Regulation of pVHL along with Down-Regulation... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog PostPublishedOct. 27, 2015, 3 p.m....23257162. Pubmed Central PMCID: PMC3552020. 10. Paladino, Simona, et al. "Protein oligomerization modulates... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog PostPublishedJune 17, 2025, 1:15 p.m....mechanisms of actions, and circulation. Frontiers in Endocrinology, 9. https://doi.org/10.3389/fendo.2018.00402... -
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog PostPublishedMay 13, 2025, 1:15 p.m.... This construct has the D64V mutation, a single amino acid change (from aspartic acid to valine at position... -
March for Science
TypeBlog PostPublishedApril 21, 2017, 2:30 p.m....seek to honor and credit women, immigrants, and minorities who have had great impacts on STEM (21–26). ... -
Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog PostPublishedFeb. 22, 2017, 3:30 p.m....mutational scanning to simultaneously test how multiple amino acid changes affect a protein of interest’s function... -
Transferable Skills Guide: Conflict Resolution
TypeBlog PostPublishedJan. 4, 2022, 2:15 p.m....I first realized I might have some minor skills in conflict resolution after college, when I discovered... -
Antibodies 101: Introduction to Immunofluorescence
TypeBlog PostPublishedNov. 16, 2021, 2:15 p.m.... References and resources References Im K, Mareninov S, Diaz MFP, Yong WH (2018) An Introduction to... -
Antibodies 101: Single Chain Fragment Variables (scFvs)
TypeBlog PostPublishedJune 3, 2021, 1:15 p.m....a flexible linker peptide that is usually 15-20 amino acids long and made up of glycine and serine with... -
Filming Science Videos in the Age of Social Distancing
TypeBlog PostPublishedMay 20, 2021, 1:15 p.m.... Quintin Marcelino... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog PostPublishedSept. 14, 2016, 2:30 p.m....and Quantification of Ribosome Inhibition by Aminoglycoside Antibiotics in Living Bacteria Using an Orthogonal... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog PostPublishedMay 10, 2016, 2:30 p.m.... PMC4649911. Khalili K, Kaminski R, Gordon J, Cosentino L, Hu W. Genome editing strategies: potential ... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog PostPublishedSept. 1, 2015, 2:30 p.m....all the major equipment, categorize all of your minor tools, like pipets, or mini-centrifuges, etc. Be... -
Hot Plasmids: Spring 2025
TypeBlog PostPublishedJune 3, 2025, 1:15 p.m....proteoglycans composed of a protein core, 2–4 glycosaminoglycans (GAG), and are (typically) tethered to the...